PMID- 29108622
OWN - NLM
STAT- MEDLINE
DCOM- 20180716
LR  - 20180716
IS  - 1879-0909 (Electronic)
IS  - 0141-0229 (Linking)
VI  - 108
DP  - 2018 Jan
TI  - Encapsulation of lipase within metal-organic framework (MOF) with enhanced 
      activity intensified under ultrasound.
PG  - 11-20
LID - S0141-0229(17)30165-5 [pii]
LID - 10.1016/j.enzmictec.2017.08.008 [doi]
AB  - The enzyme under lower-intensity ultrasonic irradiation leads to favorable 
      conformational changes, thereby enhancing its activity. In this study, lipase 
      activity was augmented upto 1.6-folds after ultrasonic treatment at 22kHz and 
      11.38Wcm(-2) for 25min. This highly activated lipase was encapsulated within 
      zeolite imidazolate framework-8 (ZIF-8) as a metal-organic framework (MOF) 
      material via facile one-step biomineralization method by simply mixing aqueous 
      solution of 2-methylimidazole (13.3mmol) and zinc acetate (1.33mmol) along with 
      sonicated lipase within 10min at room temperature (28+/-2 degrees C). The prepared 
      lipase-MOF was characterized by using FT-IR, FT-Raman, XRD, BET, confocal 
      scanning laser microscopy, TGA and SEM. Further, the thermal stability of lipase 
      embedded MOF was evaluated in the range of 55-75 degrees C on the basis of half-life 
      which showed 3.2 folds increment as against free lipase. In Michaelis-Menten 
      kinetics studies, sonicated lipase entrapped MOF showed nearly same K(m) and 
      V(max) values as that of sonicated free lipase. Moreover, the immobilized lipase 
      exhibited up to 54% of residual activity after seven successive cycles of reuse, 
      whereas it retained 90% of residual activity till twenty-five days of storage. 
      Finally, the conformational changes occurred in lipase after sonication treatment 
      and encapsulation within MOF were analyzed by using FT-IR data analysis tools and 
      fluorescent spectroscopy.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Nadar, Shamraja S
AU  - Nadar SS
AD  - Department of Chemical Engineering Institute of Chemical Technology, Matunga (E) 
      Mumbai, 400019, India.
FAU - Rathod, Virendra K
AU  - Rathod VK
AD  - Department of Chemical Engineering Institute of Chemical Technology, Matunga (E) 
      Mumbai, 400019, India. Electronic address: vk.rathod@ictmumbai.edu.in.
LA  - eng
PT  - Journal Article
DEP - 20170830
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Fungal Proteins)
RN  - 0 (Metal-Organic Frameworks)
RN  - 0 (Nanocapsules)
RN  - 1318-02-1 (Zeolites)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Aspergillus niger/enzymology
MH  - Enzyme Stability
MH  - Enzymes, Immobilized/chemistry/*metabolism
MH  - Fungal Proteins/chemistry/metabolism
MH  - Hot Temperature
MH  - Kinetics
MH  - Lipase/chemistry/*metabolism
MH  - Metal-Organic Frameworks/chemistry
MH  - Nanocapsules/chemistry/ultrastructure
MH  - Nanostructures/chemistry
MH  - Protein Structure, Secondary
MH  - Spectrometry, Fluorescence
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Ultrasonics
MH  - Zeolites/chemistry
OTO - NOTNLM
OT  - Enhanced activity
OT  - Immobilization
OT  - Lipase
OT  - Metal organic framework
OT  - Self-assembled
OT  - Ultrasound
OT  - ZIF-8
EDAT- 2017/11/08 06:00
MHDA- 2018/07/17 06:00
CRDT- 2017/11/08 06:00
PHST- 2017/06/06 00:00 [received]
PHST- 2017/08/21 00:00 [revised]
PHST- 2017/08/26 00:00 [accepted]
PHST- 2017/11/08 06:00 [entrez]
PHST- 2017/11/08 06:00 [pubmed]
PHST- 2018/07/17 06:00 [medline]
AID - S0141-0229(17)30165-5 [pii]
AID - 10.1016/j.enzmictec.2017.08.008 [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 2018 Jan;108:11-20. doi: 10.1016/j.enzmictec.2017.08.008. 
      Epub 2017 Aug 30.