PMID- 29109529
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20240314
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Nov 6
TI  - Glomerulocapillary miRNA response to HLA-class I antibody in vitro and in vivo.
PG  - 14554
LID - 10.1038/s41598-017-14674-5 [doi]
LID - 14554
AB  - Changes in miRNA expression glomerular of capillaries during antibody-mediated 
      rejection (ABMR) are poorly understood and could contribute to the deleterious 
      inflammation and fibrosis of ABMR via suppression of target genes. A better 
      understanding could lead to novel diagnostic tools and reveal novel therapeutic 
      targets. We explored deregulated miRNAs in an glomeruloendothelial in vitro model 
      of ABMR due to class I human leukocyte antigen (HLA) with and without complement 
      activation. We studied a set of 16 promising candidate miRNAs in microdissected 
      glomeruli a confirmation set of 20 human transplant biopsies (DSA+) compared to 
      10 matched controls without evidence for ABMR. Twelve out of these 16 
      glomerulocapillary miRNAs could successfully be confirmed as dysregulated in vivo 
      with 10 upregulated (let-7c-5p, miR-28-3p, miR-30d-5p, miR-99b-5p, miR-125a-5p, 
      miR-195-5p, miR-374b-3p, miR-484, miR-501-3p, miR-520e) and 2 downregulated 
      (miR29b-3p, miR-885-5p) in DSA+ vs. CONTROLS: A random forest analysis based on 
      glomerular miRNAs identified 18/20 DSA+ and 8/10 controls correctly. This 
      glomerulocapillary miRNA signature associated with HLA class I-DSA could improve 
      our understanding of ABMR and be useful for diagnostic or therapeutic purposes.
FAU - Heinemann, Falko M
AU  - Heinemann FM
AD  - Institute for Transfusion Medicine, University Hospital Essen, University 
      Duisburg-Essen, Essen, Germany.
FAU - Jindra, Peter T
AU  - Jindra PT
AD  - Immune Evaluation Laboratory, Department of Surgery, Baylor College of Medicine, 
      Houston, TX, USA.
FAU - Bockmeyer, Clemens L
AU  - Bockmeyer CL
AD  - Institute of Pathology, Department of Nephropathology, University Hospital 
      Erlangen-Nurnberg, Erlangen, Germany.
FAU - Zeuschner, Philip
AU  - Zeuschner P
AD  - Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
FAU - Wittig, Juliane
AU  - Wittig J
AD  - Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
FAU - Hoflich, Heike
AU  - Hoflich H
AD  - Institute of Pathology, Hannover Medical School, Hannover, Germany.
FAU - Esser, Marc
AU  - Esser M
AD  - Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
FAU - Abbas, Mahmoud
AU  - Abbas M
AD  - PATHOCOM, Rheine, Germany.
FAU - Dieplinger, Georg
AU  - Dieplinger G
AD  - Department of General, Visceral and Cancer Surgery, Transplant Center Cologne, 
      University of Cologne, Cologne, Germany.
FAU - Stolle, Katharina
AU  - Stolle K
AD  - Institute of Pathology, University Hospital of Cologne, Cologne, Germany.
FAU - Vester, Udo
AU  - Vester U
AD  - Children's Hospital, Pediatrics II, University of Duisburg-Essen, Essen, Germany.
FAU - Hoyer, Peter F
AU  - Hoyer PF
AD  - Children's Hospital, Pediatrics II, University of Duisburg-Essen, Essen, Germany.
FAU - Immenschuh, Stephan
AU  - Immenschuh S
AD  - Institute of Transfusion Medicine, Hannover Medical School, Hannover, Germany.
FAU - Heinold, Andreas
AU  - Heinold A
AD  - Institute for Transfusion Medicine, University Hospital Essen, University 
      Duisburg-Essen, Essen, Germany.
FAU - Horn, Peter A
AU  - Horn PA
AD  - Institute for Transfusion Medicine, University Hospital Essen, University 
      Duisburg-Essen, Essen, Germany.
FAU - Li, Wentian
AU  - Li W
AUID- ORCID: 0000-0003-1155-110X
AD  - Robert S Boas Center for Genomics and Human Genetics, Feinstein Institute for 
      Medical Research, Northwell Health, Manhasset, NY, USA.
FAU - Eisenberger, Ute
AU  - Eisenberger U
AD  - Clinic for Nephrology, University Hospital Essen, University Duisburg-Essen, 
      Essen, Germany.
FAU - Becker, Jan U
AU  - Becker JU
AD  - Institute of Pathology, University Hospital of Cologne, Cologne, Germany. 
      janbecker@gmx.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171106
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Autoantibodies)
RN  - 0 (HLA Antigens)
RN  - 0 (MicroRNAs)
SB  - IM
EIN - Sci Rep. 2018 Apr 12;8(1):6003. PMID: 29651104
MH  - Adult
MH  - Aged
MH  - Autoantibodies/*immunology
MH  - Capillaries/*metabolism
MH  - Female
MH  - Graft Rejection/immunology/metabolism
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Kidney Glomerulus/*blood supply/metabolism
MH  - Kidney Transplantation/adverse effects
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
PMC - PMC5673998
COIS- The authors declare that they have no competing interests.
EDAT- 2017/11/08 06:00
MHDA- 2019/07/06 06:00
PMCR- 2017/11/06
CRDT- 2017/11/08 06:00
PHST- 2017/02/15 00:00 [received]
PHST- 2017/10/16 00:00 [accepted]
PHST- 2017/11/08 06:00 [entrez]
PHST- 2017/11/08 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2017/11/06 00:00 [pmc-release]
AID - 10.1038/s41598-017-14674-5 [pii]
AID - 14674 [pii]
AID - 10.1038/s41598-017-14674-5 [doi]
PST - epublish
SO  - Sci Rep. 2017 Nov 6;7(1):14554. doi: 10.1038/s41598-017-14674-5.