PMID- 29110660
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20181113
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 13
IP  - 1
DP  - 2017 Nov 6
TI  - Characterization of respiratory dendritic cells from equine lung tissues.
PG  - 313
LID - 10.1186/s12917-017-1240-z [doi]
LID - 313
AB  - BACKGROUND: Dendritic cells (DCs) are professional antigen-presenting cells that 
      have multiple subpopulations with different phenotypes and immune functions. 
      Previous research demonstrated that DCs have strong potential for anti-viral 
      defense in the host. However, viruses including alphaherpesvirinae have developed 
      strategies to interfere with the function or maturation of DCs, causing immune 
      dysfunction and avoidance of pathogen elimination. The goal of the present study 
      was to isolate and characterize equine lung-derived DCs (L-DCs) for use in 
      studies of respiratory viruses and compare their features with equine 
      blood-derived DCs (B-DCs), which are currently used for these types of studies. 
      RESULTS: We found that L-DCs were morphologically similar to B-DCs. Overall, 
      B-DCs demonstrated higher expression of CD86 and CD172alpha than L-DCs, but both cell 
      types expressed high levels of MHC class II and CD44, as well as moderate amounts 
      of CD163, CD204, and Bla36. In contrast, the endocytic activity of L-DCs was 
      elevated compared to that of B-DCs. Finally, mononuclear cells isolated from lung 
      (L-MCs), which are used as precursors for L-DCs, expressed more 
      antigen-presenting cell-associated markers such as MHC class II and CD172alpha 
      compared to their counterparts from blood. CONCLUSIONS: Our results indicate that 
      L-DCs may be in an earlier differentiation stage compared to B-DCs. Concurrent 
      with this observation, L-MCs possessed significantly more antigen-uptake capacity 
      compared to their counterparts from blood. It is likely that L-DCs play an 
      important role in antigen uptake and processing of respiratory pathogens and are 
      major contributors to respiratory tract immunity and may be ideal tools for 
      future in vitro or ex vivo studies.
FAU - Lee, Yao
AU  - Lee Y
AD  - Department of Pathobiology & Diagnostic Investigation, College of Veterinary 
      Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, 
      USA.
FAU - Kiupel, Matti
AU  - Kiupel M
AD  - Department of Pathobiology & Diagnostic Investigation, College of Veterinary 
      Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, 
      USA.
FAU - Soboll Hussey, Gisela
AU  - Soboll Hussey G
AUID- ORCID: 0000-0003-1877-6926
AD  - Department of Pathobiology & Diagnostic Investigation, College of Veterinary 
      Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, 
      USA. husseygi@msu.edu.
LA  - eng
PT  - Journal Article
DEP - 20171106
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/cytology/*immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Horses
MH  - Leukocytes, Mononuclear/cytology/immunology
MH  - Lung/*cytology
MH  - Male
PMC - PMC5674750
OTO - NOTNLM
OT  - Antigen-presenting cells
OT  - Blood dendritic cells
OT  - Equine
OT  - Lung dendritic cells
COIS- CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare 
      that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains 
      neutral with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2017/11/08 06:00
MHDA- 2018/09/18 06:00
PMCR- 2017/11/06
CRDT- 2017/11/08 06:00
PHST- 2017/04/10 00:00 [received]
PHST- 2017/10/30 00:00 [accepted]
PHST- 2017/11/08 06:00 [entrez]
PHST- 2017/11/08 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
PHST- 2017/11/06 00:00 [pmc-release]
AID - 10.1186/s12917-017-1240-z [pii]
AID - 1240 [pii]
AID - 10.1186/s12917-017-1240-z [doi]
PST - epublish
SO  - BMC Vet Res. 2017 Nov 6;13(1):313. doi: 10.1186/s12917-017-1240-z.