PMID- 29111061
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20181004
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 179
DP  - 2018 Jan 1
TI  - Synthesis of fructooligosaccharides (FosA) and inulin (InuO) by GH68 
      fructosyltransferases from Bacillus agaradhaerens strain WDG185.
PG  - 350-359
LID - S0144-8617(17)31102-5 [pii]
LID - 10.1016/j.carbpol.2017.09.069 [doi]
AB  - Fructooligosaccharides (FOS) and inulin, composed of beta-2-1 linked fructose units, 
      have a broad range of industrial applications. They are known to have various 
      beneficial health effects and therefore have broad application potential in 
      nutrition. For (modified) inulin also for non-food purposes more applications are 
      arising. Examples are carboxymethylated inulin as anti-scalant and carboymlated 
      inulin as emulsifiers. Various plants synthesize FOS and/or inulin type of 
      fructans. However, isolating of FOS and inulin from plants is challenging due to 
      for instance varying chains length. There is an increasing demand for FOS and 
      inulin oligosaccharides and alternative procedures for their synthesis are 
      attractive. We identified and characterized two fructosyltransferases from 
      Bacillus agaradhaerens WDG185. FosA, a beta-fructofuranosidase, synthesises short 
      chain fructooligosaccharides (GF2-GF4) at high sucrose concentration, whereas 
      InuO, an inulosucrase, synthesises a broad range of inulooligosaccharides 
      (GF2-GF24) from sucrose, very similar to plant derived inulin. FosA and InuO 
      showed activity over a broad pH range from 6 to 10 and optimal temperature at 
      60 degrees C. Calcium ions and EDTA were found to have no effect on the activity of both 
      enzymes. Kinetic analysis showed that only at relatively low substrate 
      concentrations both enzymes showed Michaelis-Menten type of kinetics for total 
      and transglycosylation activity. Both enzymes showed increased transglycosylation 
      upon increasing substrate concentrations. These are the first examples of the 
      molecular and biochemical characterization of a beta-fructofuranosidase (FosA) and 
      an inulosucrase enzyme (InuO) and its product from a Bacillus agaradhaerens 
      strain.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Kralj, Slavko
AU  - Kralj S
AD  - DuPont Industrial Biosciences, Research and Development - Genencor International 
      B.V. Archimedesweg 30, P.O. Box 218, 2333 CN, Leiden, The Netherlands. Electronic 
      address: slavko.kralj@dupont.com.
FAU - Leeflang, Chris
AU  - Leeflang C
AD  - DuPont Industrial Biosciences, Research and Development - Genencor International 
      B.V. Archimedesweg 30, P.O. Box 218, 2333 CN, Leiden, The Netherlands.
FAU - Sierra, Estefania Ibanez
AU  - Sierra EI
AD  - DuPont Industrial Biosciences, Research and Development - Genencor International 
      B.V. Archimedesweg 30, P.O. Box 218, 2333 CN, Leiden, The Netherlands.
FAU - Kempinski, Blazej
AU  - Kempinski B
AD  - DuPont Industrial Biosciences, Research and Development - Genencor International 
      B.V. Archimedesweg 30, P.O. Box 218, 2333 CN, Leiden, The Netherlands.
FAU - Alkan, Veli
AU  - Alkan V
AD  - DuPont Industrial Biosciences, Research and Development - Genencor International 
      B.V. Archimedesweg 30, P.O. Box 218, 2333 CN, Leiden, The Netherlands.
FAU - Kolkman, Marc
AU  - Kolkman M
AD  - DuPont Industrial Biosciences, Research and Development - Genencor International 
      B.V. Archimedesweg 30, P.O. Box 218, 2333 CN, Leiden, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20170923
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Oligosaccharides)
RN  - 0 (fructooligosaccharide)
RN  - 30237-26-4 (Fructose)
RN  - 57-50-1 (Sucrose)
RN  - 9005-80-5 (Inulin)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - EC 2.4.1.- (Hexosyltransferases)
RN  - EC 2.4.1.9 (inulosucrase)
RN  - EC 3.2.1.26 (beta-Fructofuranosidase)
RN  - IY9XDZ35W2 (Glucose)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Bacillus/*enzymology/genetics
MH  - Calcium/chemistry
MH  - Edetic Acid/chemistry
MH  - Fructose/metabolism
MH  - Glucose/metabolism
MH  - Glycosylation
MH  - Hexosyltransferases/*chemistry/genetics/*isolation & purification/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Inulin/*biosynthesis
MH  - Kinetics
MH  - Oligosaccharides/metabolism
MH  - Sequence Analysis, Protein
MH  - Sucrose/metabolism
MH  - Temperature
MH  - beta-Fructofuranosidase/*biosynthesis
OTO - NOTNLM
OT  - Bacillus
OT  - Fructooligosaccharides
OT  - GH68
OT  - Inulin
OT  - Inulosucrase
OT  - beta-fructofuranosidase
EDAT- 2017/11/08 06:00
MHDA- 2018/10/03 06:00
CRDT- 2017/11/08 06:00
PHST- 2017/07/14 00:00 [received]
PHST- 2017/09/21 00:00 [revised]
PHST- 2017/09/22 00:00 [accepted]
PHST- 2017/11/08 06:00 [entrez]
PHST- 2017/11/08 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
AID - S0144-8617(17)31102-5 [pii]
AID - 10.1016/j.carbpol.2017.09.069 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2018 Jan 1;179:350-359. doi: 10.1016/j.carbpol.2017.09.069. Epub 
      2017 Sep 23.