PMID- 2911261
OWN - NLM
STAT- MEDLINE
DCOM- 19890221
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 210
IP  - 2
DP  - 1989 Feb
TI  - Detection of 6-thioguanine resistance in human peripheral blood lymphocytes (PBL) 
      of industrial workers and lung cancer patients.
PG  - 345-51
AB  - Human peripheral blood lymphocytes (PBL) were selected for 6-thioguanine (6-TG) 
      resistance in short-term (42-h) cultures in 110 high-cancer-risk industrial 
      workers, 131 primary lung cancer patients and 96 low-risk controls. The 
      lymphocytes were cultured and stimulated by phytohemagglutinin (PHA). A labeling 
      index (LI) was scored using light microscope autoradiography, based on the 
      lymphocyte's ability to incorporate tritiated thymidine with or without selective 
      agent 6-TG. The number of 6-TG-resistant cells increased in the 
      high-occupational-cancer-risk group of vinyl chloride- and mixed organic 
      industrial dust (MOID)-exposed workers as well as in the primary lung cancer 
      patients. The results were compared with the low-occupational-cancer-risk groups 
      and with samples taken from the 70 healthy individuals and 26 hospitalized, 
      non-cancerous controls. In both risk-exposed groups the frequency of 
      6-TG-resistant lymphocytes was significantly higher (p less than 0.01) than in 
      the controls. These results suggest that the original Strauss and Albertini 
      (1977, 1979) method can be used to study qualitative risk assessment in 
      carcinogen- or mutagen-exposed occupational groups.
FAU - Tompa, A
AU  - Tompa A
AD  - National Institute of Occupational Health, Budapest, Hungary.
FAU - Sapi, E
AU  - Sapi E
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Carcinogens)
RN  - 0 (Mutagens)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
RN  - FTK8U1GZNX (Thioguanine)
SB  - IM
MH  - Carcinogens/*toxicity
MH  - Drug Resistance
MH  - Humans
MH  - Hypoxanthine Phosphoribosyltransferase/deficiency/*genetics
MH  - In Vitro Techniques
MH  - Lung Neoplasms/enzymology/*genetics
MH  - Lymphocyte Activation
MH  - Lymphocytes/*drug effects
MH  - Mutagens/*toxicity
MH  - Occupational Diseases/*genetics
MH  - Thioguanine/toxicity
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
AID - 0027-5107(89)90096-1 [pii]
AID - 10.1016/0027-5107(89)90096-1 [doi]
PST - ppublish
SO  - Mutat Res. 1989 Feb;210(2):345-51. doi: 10.1016/0027-5107(89)90096-1.