PMID- 29113135
OWN - NLM
STAT- MEDLINE
DCOM- 20180629
LR  - 20181113
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 9
IP  - 11
DP  - 2017 Nov 6
TI  - Nonalcoholic Fatty Liver Disease Is Exacerbated in High-Fat Diet-Fed Gnotobiotic 
      Mice by Colonization with the Gut Microbiota from Patients with Nonalcoholic 
      Steatohepatitis.
LID - 10.3390/nu9111220 [doi]
LID - 1220
AB  - Nonalcoholic fatty liver disease (NAFLD) is a serious liver disorder associated 
      with the accumulation of fat and inflammation. The objective of this study was to 
      determine the gut microbiota composition that might influence the progression of 
      NAFLD. Germ-free mice were inoculated with feces from patients with nonalcoholic 
      steatohepatitis (NASH) or from healthy persons (HL) and then fed a standard diet 
      (STD) or high-fat diet (HFD). We found that the epididymal fat weight, hepatic 
      steatosis, multifocal necrosis, and inflammatory cell infiltration significantly 
      increased in the NASH-HFD group. These findings were consistent with markedly 
      elevated serum levels of alanine transaminase, aspartate transaminase, endotoxin, 
      interleukin 6 (IL-6), monocyte chemotactic protein 1 (Mcp1), and hepatic 
      triglycerides. In addition, the mRNA expression levels of Toll-like receptor 2 
      (Tlr2), Toll-like receptor 4 (Tlr4), tumor necrosis factor alpha (Tnf-alpha), Mcp1, 
      and peroxisome proliferator-activated receptor gamma (Ppar-gamma) significantly 
      increased. Only abundant lipid accumulation and a few inflammatory reactions were 
      observed in group HL-HFD. Relative abundance of Bacteroidetes and Firmicutes 
      shifted in the HFD-fed mice. Furthermore, the relative abundance of 
      Streptococcaceae was the highest in group NASH-HFD. Nevertheless, obesity-related 
      Lactobacillaceae were significantly upregulated in HL-HFD mice. Our results 
      revealed that the gut microbiota from NASH Patients aggravated hepatic steatosis 
      and inflammation. These findings might partially explain the NAFLD progress 
      distinctly was related to different compositions of gut microbiota.
FAU - Chiu, Chien-Chao
AU  - Chiu CC
AD  - Animal Technology Laboratories, Agricultural Technology Research Institute, 
      Miaoli 350, Taiwan. 1042032@mail.atri.org.tw.
FAU - Ching, Yung-Hao
AU  - Ching YH
AD  - Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 
      970, Taiwan. yching@mail.tcu.edu.tw.
FAU - Li, Yen-Peng
AU  - Li YP
AD  - Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, 
      Taichung 402, Taiwan. d106047002@mail.nchu.edu.tw.
FAU - Liu, Ju-Yun
AU  - Liu JY
AD  - National Laboratory Animal Center, National Applied Research Laboratories, Taipei 
      115, Taiwan. wide0720@nlac.narl.org.tw.
FAU - Huang, Yen-Te
AU  - Huang YT
AD  - National Laboratory Animal Center, National Applied Research Laboratories, Taipei 
      115, Taiwan. evan@nlac.narl.org.tw.
FAU - Huang, Yi-Wen
AU  - Huang YW
AUID- ORCID: 0000-0002-5196-7030
AD  - Liver Center, Cathay General Hospital Medical Center, Taipei 106, Taiwan. 
      yiwenhuang@ntu.edu.tw.
AD  - School of Medicine, Taipei Medical University College of Medicine, Taipei 110, 
      Taiwan. yiwenhuang@ntu.edu.tw.
FAU - Yang, Sien-Sing
AU  - Yang SS
AD  - Liver Center, Cathay General Hospital Medical Center, Taipei 106, Taiwan. 
      jaab@cgh.org.tw.
FAU - Huang, Wen-Ching
AU  - Huang WC
AD  - Department of Exercise and Health Science, National Taipei University of Nursing 
      and Health Sciences, Taipei 112, Taiwan. wenching@ntunhs.edu.tw.
FAU - Chuang, Hsiao-Li
AU  - Chuang HL
AUID- ORCID: 0000-0002-3718-9076
AD  - National Laboratory Animal Center, National Applied Research Laboratories, Taipei 
      115, Taiwan. p650214@nlac.narl.org.tw.
LA  - eng
PT  - Journal Article
DEP - 20171106
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
SB  - IM
MH  - Adipose Tissue
MH  - Animals
MH  - *Diet, High-Fat
MH  - *Gastrointestinal Microbiome
MH  - *Germ-Free Life
MH  - Humans
MH  - Liver/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Non-alcoholic Fatty Liver Disease/*microbiology
PMC - PMC5707692
OTO - NOTNLM
OT  - gut microbiota
OT  - high-fat diet
OT  - humanized gnotobiotic mice
OT  - nonalcoholic fatty liver disease
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2017/11/09 06:00
MHDA- 2018/06/30 06:00
PMCR- 2017/11/01
CRDT- 2017/11/09 06:00
PHST- 2017/08/25 00:00 [received]
PHST- 2017/10/24 00:00 [revised]
PHST- 2017/11/02 00:00 [accepted]
PHST- 2017/11/09 06:00 [entrez]
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2018/06/30 06:00 [medline]
PHST- 2017/11/01 00:00 [pmc-release]
AID - nu9111220 [pii]
AID - nutrients-09-01220 [pii]
AID - 10.3390/nu9111220 [doi]
PST - epublish
SO  - Nutrients. 2017 Nov 6;9(11):1220. doi: 10.3390/nu9111220.