PMID- 29113646
OWN - NLM
STAT- MEDLINE
DCOM- 20230926
LR  - 20230926
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 125
DP  - 2019 Aug
TI  - Effects of recombinant canine interferon-gamma injected before general anesthesia 
      with propofol and isoflurane on natural killer cytotoxic activity during 
      anesthesia in dogs.
PG  - 416-420
LID - S0034-5288(17)30100-5 [pii]
LID - 10.1016/j.rvsc.2017.10.003 [doi]
AB  - We aimed to determine whether subcutaneous injection of recombinant canine 
      interferon-gamma (rCaIFN-gamma) 1h before general anesthesia with a combination of 
      propofol and isoflurane (P-I) changes the cytotoxic activity of natural killer 
      (NK) cells during anesthesia in dogs. Nine clinically healthy beagles were 
      assigned to one of two groups: rCaIFN-gamma-treated group (n=5) and control group (no 
      treatment, n=4). One hour before P-I anesthesia, rCaIFN-gamma was subcutaneously 
      injected at a dose of 1x10(4)U/kg body weight for the test group and physiologic 
      saline was subcutaneously injected for the control group. Blood samples were 
      collected 1h before P-I anesthesia (immediately before rCaIFN-gamma injection) and 1 
      and 3h after anesthesia induction. NK cytotoxic activity was measured using the 
      Rose Bengal assay, using canine thyroid adenocarcinoma cells as target cells. In 
      the control group, NK cytotoxic activity decreased significantly over time 
      (P<0.05). On the other hand, in the rCaIFN-gamma group, the activity increased for 
      the first hour after anesthesia induction without a significant difference from 
      the level before anesthesia; furthermore, the activity at 3h after anesthesia 
      induction was comparable with that before anesthesia. NK cytotoxic activity at 1 
      and 3h after anesthesia induction was significantly higher in the rCaIFNgamma group 
      than in the control group (P<0.05). Subcutaneous injection of rCaIFN-gamma 1h before 
      P-I general anesthesia not only maintained but also enhanced NK cytotoxic 
      activity up to 3h after anesthesia induction in dogs. In conclusion, subcutaneous 
      injection of rCaIFN-gamma before cancer resection in cancer-bearing dogs might be 
      effective for preventing intraoperative dissemination of cancer cells and 
      postoperative cancer recurrence and metastasis.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Matsumoto, Hirotaka
AU  - Matsumoto H
AD  - Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, 
      School of Veterinary Medicine, Nippon Veterinary and Life Science University, 
      1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan. Electronic address: 
      matsumoto@nvlu.ac.jp.
FAU - Miyata, Takuma
AU  - Miyata T
AD  - Department of Animal Science, Teikyo University of Science, 2-2-1 Senjusakuragi, 
      Adachi-ku, Tokyo 120-0045, Japan.
FAU - Ohkusa, Tomoko
AU  - Ohkusa T
AD  - Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, 
      School of Veterinary Medicine, Nippon Veterinary and Life Science University, 
      1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
FAU - Teshima, Takahiro
AU  - Teshima T
AD  - Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, 
      School of Veterinary Medicine, Nippon Veterinary and Life Science University, 
      1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
FAU - Koyama, Hidekazu
AU  - Koyama H
AD  - Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, 
      School of Veterinary Medicine, Nippon Veterinary and Life Science University, 
      1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan.
LA  - eng
PT  - Clinical Trial, Veterinary
PT  - Journal Article
DEP - 20171026
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Hypnotics and Sedatives)
RN  - 82115-62-6 (Interferon-gamma)
RN  - CYS9AKD70P (Isoflurane)
RN  - YI7VU623SF (Propofol)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - Anesthesia, General/veterinary
MH  - Anesthetics, Inhalation/administration & dosage/pharmacology
MH  - Hypnotics and Sedatives/administration & dosage/pharmacology
MH  - *Interferon-gamma/administration & dosage/pharmacology
MH  - *Isoflurane/administration & dosage/pharmacology
MH  - Killer Cells, Natural/drug effects/physiology
MH  - Postoperative Period
MH  - *Propofol/administration & dosage/pharmacology
MH  - Recombinant Proteins/pharmacology
OTO - NOTNLM
OT  - Cytotoxic activity
OT  - Dog
OT  - IFN-gamma
OT  - Isoflurane
OT  - NK
OT  - Propofol
EDAT- 2017/11/09 06:00
MHDA- 2019/12/04 06:00
CRDT- 2017/11/09 06:00
PHST- 2017/01/26 00:00 [received]
PHST- 2017/10/17 00:00 [revised]
PHST- 2017/10/25 00:00 [accepted]
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2017/11/09 06:00 [entrez]
AID - S0034-5288(17)30100-5 [pii]
AID - 10.1016/j.rvsc.2017.10.003 [doi]
PST - ppublish
SO  - Res Vet Sci. 2019 Aug;125:416-420. doi: 10.1016/j.rvsc.2017.10.003. Epub 2017 Oct 
      26.