PMID- 29114353
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 11
IP  - 114
DP  - 2017
TI  - A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis 
      (Linnaeus, 1758) (Heteroptera, Nepidae).
PG  - 641-657
LID - 10.3897/CompCytogen.v11i4.14928 [doi]
AB  - An account is given of the karyotypes and male meiosis of the Water Scorpion Nepa 
      cinerea Linnaeus, 1758 and the Water Stick Insect Ranatra linearis (Linnaeus, 
      1758) (Heteroptera, Nepomorpha, Nepidae). A number of different approaches and 
      techniques were tried: the employment of both male and female gonads and mid-guts 
      as the sources of chromosomes, squash and air-drying methods for chromosome 
      preparations, C-banding and fluorescence in situ hybridization (FISH) for 
      chromosome study. We found that N. cinerea had a karyotype comprising 14 pairs of 
      autosomes and a multiple sex chromosome system, which is X(1)X(2)X(3)X(4)Y (male symbol) / 
      X(1)X(1)X(2)X(2)X(3)X(3)X(4)X(4) (female symbol), whereas R. linearis had a karyotype 
      comprising 19 pairs of autosomes and a multiple sex chromosome system 
      X(1)X(2)X(3)X(4)Y (male symbol) / X(1)X(1)X(2)X(2)X(3)X(3)X(4)X(4) (female symbol). In both N. cinerea 
      and R. linearis, the autosomes formed chiasmate bivalents in spermatogenesis, and 
      the sex chromosome univalents divided during the first meiotic division and 
      segregated during the second one suggesting thus a post-reductional type of 
      behaviour. These results confirm and amplify those of Steopoe (1925, 1927, 1931, 
      1932) but are inconsistent with those of other researchers. C-banding appeared 
      helpful in pairing up the autosomes for karyotype assembly; however in R. 
      linearis the chromosomes were much more uniform in size and general appearance 
      than in N. cinerea. FISH for 18S ribosomal DNA (major rDNA) revealed 
      hybridization signals on two of the five sex chromosomes in N. cinerea. In R. 
      linearis, rDNA location was less obvious than in N. cinerea; however it is 
      suggested to be similar. We have detected the presence of the canonical "insect" 
      (TTAGG) (n) telomeric repeat in chromosomes of these species. This is the first 
      application of C-banding and FISH in the family Nepidae.
FAU - Angus, Robert B
AU  - Angus RB
AD  - Department of Life Sciences (Insects), The Natural History Museum, Cromwell Road, 
      London SW7 5BD, UK.
FAU - Jeangirard, Constance
AU  - Jeangirard C
AD  - School of Biological Sciences, Royal Holloway University of London, Egham, Surrey 
      TW20 0EX, UK.
FAU - Stoianova, Desislava
AU  - Stoianova D
AD  - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 
      1 Tsar Osvoboditel, Sofia 1000, Bulgaria.
FAU - Grozeva, Snejana
AU  - Grozeva S
AD  - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 
      1 Tsar Osvoboditel, Sofia 1000, Bulgaria.
FAU - Kuznetsova, Valentina G
AU  - Kuznetsova VG
AD  - Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, St. 
      Petersburg 199034, Russia.
LA  - eng
PT  - Journal Article
DEP - 20170914
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC5672273
OTO - NOTNLM
OT  - (TTAGG)n
OT  - 18S rDNA
OT  - C-banding
OT  - FISH
OT  - Heteroptera
OT  - Karyotype
OT  - Nepa cinerea
OT  - Nepomorpha
OT  - Ranatra linearis
OT  - male meiosis
EDAT- 2017/11/09 06:00
MHDA- 2017/11/09 06:01
PMCR- 2017/09/14
CRDT- 2017/11/09 06:00
PHST- 2017/06/07 00:00 [received]
PHST- 2017/08/13 00:00 [accepted]
PHST- 2017/11/09 06:00 [entrez]
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2017/11/09 06:01 [medline]
PHST- 2017/09/14 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v11i4.14928 [doi]
PST - epublish
SO  - Comp Cytogenet. 2017 Sep 14;11(114):641-657. doi: 
      10.3897/CompCytogen.v11i4.14928. eCollection 2017.