PMID- 29114390
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
IS  - 2662-6810 (Print)
IS  - 2052-7276 (Electronic)
IS  - 2052-7276 (Linking)
VI  - 4
DP  - 2017
TI  - Phospholipase D inhibition by hexanal is associated with calcium signal 
      transduction events in raspberry.
PG  - 17042
LID - 10.1038/hortres.2017.42 [doi]
AB  - Raspberry (Rubus spp.) is an economically important crop with a restricted 
      growing season and very limited fruit shelf-life due to its extreme tenderness. 
      In order to prolong its shelf life, an aqueous composition containing hexanal as 
      the key active ingredient (HC) was applied as a preharvest spray during fruit 
      development. The effects of HC were assessed using physiological, biochemical and 
      anatomical parameters on the treated fruits and compared with the effects of mock 
      inoculation which lacked hexanal. Sugars and acidity did not show a significant 
      change in response to HC treatment, while the pulling force (the tension required 
      to detach the berry from the receptacle) significantly improved in the HC-treated 
      fruits, compared to control. Scanning electron microscope (SEM) analysis revealed 
      a high correlation between the presence of rigid epidermal hairs and a stronger 
      degree of attachment between berries and their receptacle in the HC treated 
      fruits. Further, electron micrographs also showed abnormal crystalline 
      depositions on the epidermal drupelets of the treated berries. Energy Dispersive 
      X-ray Spectroscopy (EDS) analysis showed those crystals to be largely composed of 
      calcium. HC treatment also resulted in the reduction of transcript level of three 
      phospholipase D genes, as well as altered expression pattern of five members of 
      the annexin gene family, and four calmodulin-binding transcription activators. 
      Quantification of PLD activity showed that hexanal inhibited PLD activity in 
      treated berries. The potential crosstalk between hexanal, phospholipase D 
      activity and calcium and this crosstalk's role in delaying fruit softening and in 
      prolonging storage life of fruits shelf life is discussed.
FAU - Kayal, Walid El
AU  - Kayal WE
AD  - Department of Plant Agriculture, University of Guelph-Vineland Station, 4890 
      Victoria Avenue N, Vineland, Ontario L0R2E0, Canada.
FAU - Paliyath, Gopinadhan
AU  - Paliyath G
AD  - Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G2W1, 
      Canada.
FAU - Sullivan, J Alan
AU  - Sullivan JA
AD  - Department of Plant Agriculture, University of Guelph, Guelph, Ontario N1G2W1, 
      Canada.
FAU - Subramanian, Jayasankar
AU  - Subramanian J
AD  - Department of Plant Agriculture, University of Guelph-Vineland Station, 4890 
      Victoria Avenue N, Vineland, Ontario L0R2E0, Canada.
LA  - eng
PT  - Journal Article
DEP - 20170913
PL  - England
TA  - Hortic Res
JT  - Horticulture research
JID - 101655540
PMC - PMC5596117
COIS- The authors declare no conflict of interest.
EDAT- 2017/11/09 06:00
MHDA- 2017/11/09 06:01
PMCR- 2017/09/13
CRDT- 2017/11/09 06:00
PHST- 2017/05/23 00:00 [received]
PHST- 2017/07/04 00:00 [revised]
PHST- 2017/07/05 00:00 [accepted]
PHST- 2017/11/09 06:00 [entrez]
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2017/11/09 06:01 [medline]
PHST- 2017/09/13 00:00 [pmc-release]
AID - hortres201742 [pii]
AID - 10.1038/hortres.2017.42 [doi]
PST - epublish
SO  - Hortic Res. 2017 Sep 13;4:17042. doi: 10.1038/hortres.2017.42. eCollection 2017.