PMID- 29115123
OWN - NLM
STAT- MEDLINE
DCOM- 20180102
LR  - 20180102
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 65
IP  - 48
DP  - 2017 Dec 6
TI  - Structural Characterization of a Tetrapeptide from Sesame Flavor-Type Baijiu and 
      Its Preventive Effects against AAPH-Induced Oxidative Stress in HepG2 Cells.
PG  - 10495-10504
LID - 10.1021/acs.jafc.7b04815 [doi]
AB  - Peptides are rarely reported from Chinese Baijiu (Chinese liquor) because they 
      are often present in very low concentrations in the complex matrix. A 
      tetrapeptide, Ala-Lys-Arg-Ala (AKRA), was recently identified by high-performance 
      liquid chromatography and quadrupole-time-of-flight-mass spectrometry 
      (HPLC-Q-TOF-MS) from sesame flavor-type Baijiu at a concentration of 8.497 +/- 
      0.753 mug/L (P > 0.05), and this tetrapeptide showed preventive effects against 
      2,2'-azobis(2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative 
      stress in HepG2 cells. The cellular antioxidant activity assay results showed 
      that AKRA protected AAPH-induced oxidative stress in HepG2 cells in a 
      concentration-dependent manner. Pretreatment of the cells for 2 h with AKRA 
      (0.38-1.50 mg/mL) caused strong intracellular reactive oxygen species (ROS) 
      scavenging activities and prevented a decrease in reduced glutathione (GSH) and 
      increases in oxidized glutathione (GSSG) and malondialdehyde (MDA). In addition, 
      AKRA treatment prevented significant decreases in glutathione peroxidase 
      (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) induced by AAPH. Thus, 
      AKRA treatment ameliorated AAPH-induced oxidative stress in HepG2 cells. This 
      study will be important for the design and regulation of functional Baijiu 
      production.
FAU - Wu, Jihong
AU  - Wu J
AD  - School of Food Science and Engineering, South China University of Technology , 
      Guangzhou, China 510640.
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Technology and Business University , Beijing, China 100048.
FAU - Huo, Jiaying
AU  - Huo J
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Technology and Business University , Beijing, China 100048.
FAU - Huang, Mingquan
AU  - Huang M
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Technology and Business University , Beijing, China 100048.
FAU - Zhao, Mouming
AU  - Zhao M
AUID- ORCID: 0000-0003-0221-3838
AD  - School of Food Science and Engineering, South China University of Technology , 
      Guangzhou, China 510640.
FAU - Luo, Xuelian
AU  - Luo X
AD  - State Key Laboratory of Infectious Disease Prevention and Control, National 
      Institute for Communicable Disease Control and Prevention, Collaborative 
      Innovation Center for Diagnosis and Treatment of Infectious Disease, Chinese 
      Center for Disease Control and Prevention , Beijing, China 102206.
FAU - Sun, Baoguo
AU  - Sun B
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing 
      Technology and Business University , Beijing, China 100048.
LA  - eng
PT  - Journal Article
DEP - 20171117
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Amidines)
RN  - 0 (Antioxidants)
RN  - 0 (Flavoring Agents)
RN  - 0 (Peptides)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 7381JDR72F (2,2'-azobis(2-amidinopropane))
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Amidines/toxicity
MH  - Antioxidants/metabolism
MH  - Catalase/metabolism
MH  - Flavoring Agents/*chemistry/pharmacology
MH  - Glutathione/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Mass Spectrometry
MH  - Oxidative Stress/*drug effects
MH  - Peptides/*chemistry/*pharmacology
MH  - Plant Extracts/*chemistry/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Sesamum/*chemistry
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - HPLC-Q-TOF-MS
OT  - HepG2 cell
OT  - antioxidant peptides
OT  - oxidative stress
OT  - sesame flavor-type Baijiu
EDAT- 2017/11/09 06:00
MHDA- 2018/01/03 06:00
CRDT- 2017/11/09 06:00
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
PHST- 2017/11/09 06:00 [entrez]
AID - 10.1021/acs.jafc.7b04815 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2017 Dec 6;65(48):10495-10504. doi: 10.1021/acs.jafc.7b04815. 
      Epub 2017 Nov 17.