PMID- 29117588
OWN - NLM
STAT- MEDLINE
DCOM- 20180212
LR  - 20181202
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 192
DP  - 2018 Feb
TI  - Metabolic profiling on the effect of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) 
      in MCF-7 cells.
PG  - 297-304
LID - S0045-6535(17)31756-3 [pii]
LID - 10.1016/j.chemosphere.2017.10.170 [doi]
AB  - Polybrominated diphenyl ethers (PBDEs) are commonly used to prevent the 
      development of fire in various factory products. Due to the adverse effects on 
      human health and the bio-accumulation capacity, PBDEs are considered as one kind 
      of persistent organic pollutants (POPs). BDE-47 is one of the most frequently 
      detected PBDEs congeners in human samples. Although numerous studies have shown 
      the close connection between BDE-47 and human health, few reports were related to 
      breast carcinoma. In the present study, the toxicity mechanism of BDE-47 was 
      investigated by using MCF-7 breast cancer cells. Metabolomics analysis was 
      conducted by using ultra-high performance liquid chromatography coupled with mass 
      spectrometry (UHPLC-MS). Results showed that the toxicity to MCF-7 cells 
      gradually increased when the concentration of BDE-47 exceeded 1 muM in the medium 
      with 1% fetal bovine serum (FBS). It was found that pyrimidine metabolism, purine 
      metabolism and pentose phosphate pathway (PPP) were the most influenced metabolic 
      pathways, and the metabolites in the three metabolic pathways were significantly 
      downregulated. Moreover, the increase of reactive oxygen species (ROS) was 
      detected by using the 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) 
      staining method. The obtained results suggested that the BDE-47 induced oxidative 
      stress by downregulating the NADPH generation in PPP. The pyrimidine metabolism 
      and purine metabolism might be downregulated by the downregulation of mRNA 
      transcripts. Therefore, BDE-47 could induce oxidative stress by inhibiting PPP 
      and disorder the metabolism of the entire cell subsequently. This research 
      provided evidence for investigating mechanism of the adverse effect of BDE-47 on 
      human health.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Wei, Juntong
AU  - Wei J
AD  - State Key Laboratory of Environmental and Biological Analysis, Department of 
      Chemistry, Hong Kong Baptist University, Hong Kong Special Administrative Region.
FAU - Xiang, Li
AU  - Xiang L
AD  - State Key Laboratory of Environmental and Biological Analysis, Department of 
      Chemistry, Hong Kong Baptist University, Hong Kong Special Administrative Region.
FAU - Yuan, Zigao
AU  - Yuan Z
AD  - Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen, Tsinghua 
      University, Shenzhen, China.
FAU - Li, Shangfu
AU  - Li S
AD  - Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen, Tsinghua 
      University, Shenzhen, China.
FAU - Yang, Chunxue
AU  - Yang C
AD  - State Key Laboratory of Environmental and Biological Analysis, Department of 
      Chemistry, Hong Kong Baptist University, Hong Kong Special Administrative Region.
FAU - Liu, Hongxia
AU  - Liu H
AD  - Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen, Tsinghua 
      University, Shenzhen, China.
FAU - Jiang, Yuyang
AU  - Jiang Y
AD  - Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen, Tsinghua 
      University, Shenzhen, China.
FAU - Cai, Zongwei
AU  - Cai Z
AD  - State Key Laboratory of Environmental and Biological Analysis, Department of 
      Chemistry, Hong Kong Baptist University, Hong Kong Special Administrative Region. 
      Electronic address: zwcai@hkbu.edu.hk.
LA  - eng
PT  - Journal Article
DEP - 20171031
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Reactive Oxygen Species)
RN  - 0F5N573A2Y (Ether)
RN  - 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
SB  - IM
MH  - Breast Neoplasms/pathology
MH  - Ether
MH  - Halogenated Diphenyl Ethers/metabolism/*toxicity
MH  - Humans
MH  - MCF-7 Cells
MH  - Mass Spectrometry
MH  - Metabolic Networks and Pathways/drug effects
MH  - Metabolomics/methods
MH  - Oxidative Stress/drug effects
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - BDE-47
OT  - Breast cancer
OT  - Metabolomics
OT  - NADPH
OT  - ROS
EDAT- 2017/11/09 06:00
MHDA- 2018/02/13 06:00
CRDT- 2017/11/09 06:00
PHST- 2017/08/19 00:00 [received]
PHST- 2017/10/25 00:00 [revised]
PHST- 2017/10/30 00:00 [accepted]
PHST- 2017/11/09 06:00 [pubmed]
PHST- 2018/02/13 06:00 [medline]
PHST- 2017/11/09 06:00 [entrez]
AID - S0045-6535(17)31756-3 [pii]
AID - 10.1016/j.chemosphere.2017.10.170 [doi]
PST - ppublish
SO  - Chemosphere. 2018 Feb;192:297-304. doi: 10.1016/j.chemosphere.2017.10.170. Epub 
      2017 Oct 31.