PMID- 29118432
OWN - NLM
STAT- MEDLINE
DCOM- 20190708
LR  - 20190708
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Nov 8
TI  - Novel method for rapid fluorescence in-situ hybridization of ALK rearrangement 
      using non-contact alternating current electric field mixing.
PG  - 15116
LID - 10.1038/s41598-017-15515-1 [doi]
LID - 15116
AB  - Echinoderm microtubule-associated protein-like 4 gene and anaplastic lymphoma 
      kinase gene (EML4-ALK) rearrangement is a key driver mutation in non-small cell 
      lung cancer (NSCLC). Although Break-Apart ALK fluorescence in situ hybridization 
      (FISH) is a reliable diagnostic method for detecting ALK gene rearrangement, it 
      is too costly and time-consuming for use as a routine screening test. Our aim was 
      to evaluate the clinical utility of a novel rapid FISH (RaFISH) method developed 
      to facilitate hybridization. RaFISH takes advantage of the non-contact mixing 
      effect of an alternating current (AC) electric field. Eighty-five specimens were 
      used from patients diagnosed with NSCLC identified immunohistochemically as ALK 
      0, (1/2+) or (3+). With RaFISH, the ALK test was completed within 4.5 h, as 
      compared to 20 h needed for the standard FISH. Although RaFISH produced results 
      more promptly, the staining and accuracy of the ALK evaluation with RaFISH was 
      equal to the standard. We found 97.6% agreement between FISH and RaFISH based on 
      the status of the ALK signals. These results suggest RaFISH could be used as a 
      clinical tool to promptly determine ALK status.
FAU - Fujishima, Satoshi
AU  - Fujishima S
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Imai, Kazuhiro
AU  - Imai K
AUID- ORCID: 0000-0003-4490-7862
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan. i-karo@mui.biglobe.ne.jp.
FAU - Nakamura, Ryuta
AU  - Nakamura R
AD  - Akita Industrial Technology Center, Akita, Japan.
FAU - Nanjo, Hiroshi
AU  - Nanjo H
AD  - Division of Clinical Pathology, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Saito, Yoshitaro
AU  - Saito Y
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Saito, Hajime
AU  - Saito H
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Terata, Kaori
AU  - Terata K
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Sato, Yusuke
AU  - Sato Y
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Motoyama, Satoru
AU  - Motoyama S
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Akagami, Yoichi
AU  - Akagami Y
AD  - Akita Industrial Technology Center, Akita, Japan.
FAU - Minamiya, Yoshihiro
AU  - Minamiya Y
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171108
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 3.4.21.- (EML4 protein, human)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Aged
MH  - Anaplastic Lymphoma Kinase/*genetics/metabolism
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/metabolism/pathology
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - Electrochemical Techniques/*methods
MH  - Female
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Microtubule-Associated Proteins/*genetics/metabolism
MH  - Middle Aged
MH  - Mutation
MH  - Reproducibility of Results
MH  - Serine Endopeptidases/*genetics/metabolism
PMC - PMC5678187
COIS- The authors declare that they have no competing interests.
EDAT- 2017/11/10 06:00
MHDA- 2019/07/10 06:00
PMCR- 2017/11/08
CRDT- 2017/11/10 06:00
PHST- 2017/08/02 00:00 [received]
PHST- 2017/10/27 00:00 [accepted]
PHST- 2017/11/10 06:00 [entrez]
PHST- 2017/11/10 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
PHST- 2017/11/08 00:00 [pmc-release]
AID - 10.1038/s41598-017-15515-1 [pii]
AID - 15515 [pii]
AID - 10.1038/s41598-017-15515-1 [doi]
PST - epublish
SO  - Sci Rep. 2017 Nov 8;7(1):15116. doi: 10.1038/s41598-017-15515-1.