PMID- 29123131
OWN - NLM
STAT- MEDLINE
DCOM- 20180907
LR  - 20181113
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 8
IP  - 1
DP  - 2017 Nov 9
TI  - Influenza virus genome reaches the plasma membrane via a modified endoplasmic 
      reticulum and Rab11-dependent vesicles.
PG  - 1396
LID - 10.1038/s41467-017-01557-6 [doi]
LID - 1396
AB  - Transport of neo-synthesized influenza A virus (IAV) viral ribonucleoproteins 
      (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the precise 
      mechanism remains poorly understood. We used metal-tagging and immunolabeling to 
      visualize viral proteins and cellular endomembrane markers by electron microscopy 
      of IAV-infected cells. Unexpectedly, we provide evidence that the vRNP components 
      and the Rab11 protein are present at the membrane of a modified, tubulated 
      endoplasmic reticulum (ER) that extends all throughout the cell, and on 
      irregularly coated vesicles (ICVs). Some ICVs are found very close to the ER and 
      to the plasma membrane. ICV formation is observed only in infected cells and 
      requires an active Rab11 GTPase. Against the currently accepted model in which 
      vRNPs are carried onto Rab11-positive recycling endosomes across the cytoplasm, 
      our findings reveal that the endomembrane organelle that is primarily involved in 
      the transport of vRNPs is the ER.
FAU - de Castro Martin, Isabel Fernandez
AU  - de Castro Martin IF
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular 
      Structures, Cell Structure Lab, 28049, Madrid, Spain.
FAU - Fournier, Guillaume
AU  - Fournier G
AD  - Departement de Virologie, Institut Pasteur, Unite de Genetique Moleculaire des 
      Virus a ARN, 75015, Paris, France.
AD  - CNRS, UMR3569, 75015, Paris, France.
AD  - Universite Paris Diderot, Sorbonne Paris Cite, EA302, 75015, Paris, France.
FAU - Sachse, Martin
AU  - Sachse M
AD  - Center for Innovation and Technological Research, Ultrastructural Bio-imaging, 
      Institut Pasteur, 75015, Paris, France.
FAU - Pizarro-Cerda, Javier
AU  - Pizarro-Cerda J
AD  - Departement de Biologie Cellulaire et Infection, Institut Pasteur, Unite des 
      Interactions Bacteries-Cellules, 75015, Paris, France.
AD  - INSERM, U220 8. INRA, USC 2020, 75015, Paris, France.
AD  - Unite de Recherche Yersinia, Institut Pasteur, 75015, Paris, France.
AD  - Centre National de Reference 'Peste et autres Yersinioses', 75015, Paris, France.
AD  - Centre Collaborateur OMS de Reference et Recherche 'Yersinioses', Institut 
      Pasteur, Unite des Interactions Bacteries-Cellules, 75015, Paris, France.
FAU - Risco, Cristina
AU  - Risco C
AD  - Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular 
      Structures, Cell Structure Lab, 28049, Madrid, Spain. crisco@cnb.csic.es.
FAU - Naffakh, Nadia
AU  - Naffakh N
AD  - Departement de Virologie, Institut Pasteur, Unite de Genetique Moleculaire des 
      Virus a ARN, 75015, Paris, France. nadia.naffakh@pasteur.fr.
AD  - CNRS, UMR3569, 75015, Paris, France. nadia.naffakh@pasteur.fr.
AD  - Universite Paris Diderot, Sorbonne Paris Cite, EA302, 75015, Paris, France. 
      nadia.naffakh@pasteur.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171109
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Viral Proteins)
RN  - EC 3.6.1.- (rab11 protein)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Cell Membrane/metabolism
MH  - Dogs
MH  - Endoplasmic Reticulum/*metabolism
MH  - Endosomes/metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Influenza A virus/*genetics
MH  - Madin Darby Canine Kidney Cells
MH  - Microscopy, Electron
MH  - Protein Transport/*physiology
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Ribonucleoproteins/*metabolism
MH  - Viral Proteins/*metabolism
MH  - rab GTP-Binding Proteins/genetics/*metabolism
PMC - PMC5680169
COIS- The authors declare no competing financial interests.
EDAT- 2017/11/11 06:00
MHDA- 2018/09/08 06:00
PMCR- 2017/11/09
CRDT- 2017/11/11 06:00
PHST- 2017/01/13 00:00 [received]
PHST- 2017/09/27 00:00 [accepted]
PHST- 2017/11/11 06:00 [entrez]
PHST- 2017/11/11 06:00 [pubmed]
PHST- 2018/09/08 06:00 [medline]
PHST- 2017/11/09 00:00 [pmc-release]
AID - 10.1038/s41467-017-01557-6 [pii]
AID - 1557 [pii]
AID - 10.1038/s41467-017-01557-6 [doi]
PST - epublish
SO  - Nat Commun. 2017 Nov 9;8(1):1396. doi: 10.1038/s41467-017-01557-6.