PMID- 29124858
OWN - NLM
STAT- MEDLINE
DCOM- 20190509
LR  - 20190509
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 20
IP  - 2
DP  - 2018 Feb
TI  - High dark inorganic carbon fixation rates by specific microbial groups in the 
      Atlantic off the Galician coast (NW Iberian margin).
PG  - 602-611
LID - 10.1111/1462-2920.13984 [doi]
AB  - Bulk dark dissolved inorganic carbon (DIC) fixation rates were determined and 
      compared to microbial heterotrophic production in subsurface, meso- and 
      bathypelagic Atlantic waters off the Galician coast (NW Iberian margin). DIC 
      fixation rates were slightly higher than heterotrophic production throughout the 
      water column, however, more prominently in the bathypelagic waters. 
      Microautoradiography combined with catalyzed reporter deposition fluorescence in 
      situ hybridization (MICRO-CARD-FISH) allowed us to identify several microbial 
      groups involved in dark DIC uptake. The contribution of SAR406 (Marinimicrobia), 
      SAR324 (Deltaproteobacteria) and Alteromonas (Gammaproteobacteria) to the dark 
      DIC fixation was significantly higher than that of SAR202 (Chloroflexi) and 
      Thaumarchaeota, in agreement with their contribution to microbial abundance. 
      Q-PCR on the gene encoding for the ammonia monooxygenase subunit A (amoA) from 
      the putatively high versus low ammonia concentration ecotypes revealed their 
      depth-stratified distribution pattern. Taken together, our results indicate that 
      chemoautotrophy is widespread among microbes in the dark ocean, particularly in 
      bathypelagic waters. This chemolithoautotrophic biomass production in the dark 
      ocean, depleted in bio-available organic matter, might play a substantial role in 
      sustaining the dark ocean's food web.
CI  - (c) 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Guerrero-Feijoo, Elisa
AU  - Guerrero-Feijoo E
AD  - IEO, Instituto Espanol de Oceanografia, Centro Oceanografico de A Coruna, Apdo 
      130, 15080 A Coruna, Spain.
FAU - Sintes, Eva
AU  - Sintes E
AD  - Department of Limnology and Bio-Oceanography, University of Vienna, Center of 
      Ecology, Austria.
FAU - Herndl, Gerhard J
AU  - Herndl GJ
AD  - Department of Limnology and Bio-Oceanography, University of Vienna, Center of 
      Ecology, Austria.
AD  - Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands 
      Institute for Sea Research, Utrecht University, PO Box 59, 1790 AB Den Burg, The 
      Netherlands.
FAU - Varela, Marta M
AU  - Varela MM
AD  - IEO, Instituto Espanol de Oceanografia, Centro Oceanografico de A Coruna, Apdo 
      130, 15080 A Coruna, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171207
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 7440-44-0 (Carbon)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/metabolism
MH  - Archaea/metabolism
MH  - Atlantic Ocean
MH  - Bacteria/*metabolism
MH  - Carbon/*metabolism
MH  - *Carbon Cycle
MH  - Chemoautotrophic Growth
MH  - Chloroflexi/metabolism
MH  - Deltaproteobacteria/metabolism
MH  - Europe
MH  - Gammaproteobacteria/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Oxidoreductases/genetics
MH  - Seawater/*microbiology
EDAT- 2017/11/11 06:00
MHDA- 2019/05/10 06:00
CRDT- 2017/11/11 06:00
PHST- 2017/05/08 00:00 [received]
PHST- 2017/10/30 00:00 [accepted]
PHST- 2017/11/11 06:00 [pubmed]
PHST- 2019/05/10 06:00 [medline]
PHST- 2017/11/11 06:00 [entrez]
AID - 10.1111/1462-2920.13984 [doi]
PST - ppublish
SO  - Environ Microbiol. 2018 Feb;20(2):602-611. doi: 10.1111/1462-2920.13984. Epub 
      2017 Dec 7.