PMID- 29126343 OWN - NLM STAT- MEDLINE DCOM- 20190403 LR - 20190403 IS - 1365-3040 (Electronic) IS - 0140-7791 (Linking) VI - 41 IP - 5 DP - 2018 May TI - Oxidative stress-triggered interactions between the succinyl- and acetyl-proteomes of rice leaves. PG - 1139-1153 LID - 10.1111/pce.13100 [doi] AB - Protein lysine acylations, such as succinylation and acetylation, are important post-translational modification (PTM) mechanisms, with key roles in cellular regulation. Antibody-based affinity enrichment, high-resolution liquid chromatography mass spectrometry analysis, and integrated bioinformatics analysis were used to characterize the lysine succinylome (K(suc) ) and acetylome (K(ace) ) of rice leaves. In total, 2,593 succinylated and 1,024 acetylated proteins were identified, of which 723 were simultaneously acetylated and succinylated. Proteins involved in photosynthetic carbon metabolism such as the large and small subunits of RuBisCO, ribosomal functions, and other key processes were subject to both PTMs. Preliminary insights into oxidant-induced changes to the rice acetylome and succinylome were gained from treatments with hydrogen peroxide. Exposure to oxidative stress did not regulate global changes in the rice acetylome or succinylome but rather led to modifications on a specific subset of the identified sites. De-succinylation of recombinant catalase (CATA) and glutathione S-transferase (OsGSTU6) altered the activities of these enzymes showing that this PTM may have a regulatory function. These findings not only greatly extend the list of acetylated and/or succinylated proteins but they also demonstrate the close cooperation between these PTMs in leaf proteins with key metabolic functions. CI - (c) 2017 John Wiley & Sons Ltd. FAU - Zhou, Heng AU - Zhou H AD - Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Finkemeier, Iris AU - Finkemeier I AD - Institute of Plant Biology and Biotechnology, Westfaelische Wilhelms University Muenster, Muenster, 48149, Germany. FAU - Guan, Wenxue AU - Guan W AD - Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Tossounian, Maria-Armineh AU - Tossounian MA AD - VIB-VUB Center for Structural Biology, Brussels, B-1050, Belgium. AD - Brussels Center for Redox Biology, Brussels, B-1050, Belgium. AD - Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, B-1050, Belgium. FAU - Wei, Bo AU - Wei B AD - VIB-VUB Center for Structural Biology, Brussels, B-1050, Belgium. AD - Brussels Center for Redox Biology, Brussels, B-1050, Belgium. AD - Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, B-1050, Belgium. AD - VIB-UGent Center for Plant Systems Biology, Technologiepark 927, Ghent, B-9052, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent, B-9052, Belgium. FAU - Young, David AU - Young D AD - VIB-VUB Center for Structural Biology, Brussels, B-1050, Belgium. AD - Brussels Center for Redox Biology, Brussels, B-1050, Belgium. AD - Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, B-1050, Belgium. FAU - Huang, Jingjing AU - Huang J AD - VIB-VUB Center for Structural Biology, Brussels, B-1050, Belgium. AD - Brussels Center for Redox Biology, Brussels, B-1050, Belgium. AD - Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, B-1050, Belgium. AD - VIB-UGent Center for Plant Systems Biology, Technologiepark 927, Ghent, B-9052, Belgium. AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent, B-9052, Belgium. FAU - Messens, Joris AU - Messens J AD - VIB-VUB Center for Structural Biology, Brussels, B-1050, Belgium. AD - Brussels Center for Redox Biology, Brussels, B-1050, Belgium. AD - Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, B-1050, Belgium. FAU - Yang, Xibin AU - Yang X AD - Jingjie PTM Biolab (Hangzhou) Co. Ltd., Hangzhou, 310018, China. FAU - Zhu, Jun AU - Zhu J AD - Jingjie PTM Biolab (Hangzhou) Co. Ltd., Hangzhou, 310018, China. FAU - Wilson, Michael H AU - Wilson MH AD - Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK. FAU - Shen, Wenbiao AU - Shen W AD - Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Xie, Yanjie AU - Xie Y AUID- ORCID: 0000-0002-3503-1267 AD - Laboratory Center of Life Sciences, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China. AD - Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK. FAU - Foyer, Christine H AU - Foyer CH AUID- ORCID: 0000-0001-5989-6989 AD - Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180109 PL - United States TA - Plant Cell Environ JT - Plant, cell & environment JID - 9309004 RN - 0 (Plant Proteins) RN - 0 (Proteome) RN - AB6MNQ6J6L (Succinic Acid) RN - K3Z4F929H6 (Lysine) SB - IM MH - Acetylation MH - Aminoacylation MH - Chromatography, Liquid MH - *Gene Expression Regulation, Plant MH - Lysine/*metabolism MH - Mass Spectrometry MH - Oryza/*physiology MH - Oxidative Stress MH - Photosynthesis MH - Plant Leaves/physiology MH - Plant Proteins/genetics/*metabolism MH - *Protein Processing, Post-Translational MH - *Proteome MH - Succinic Acid/metabolism OTO - NOTNLM OT - catalase OT - glutathione S transferase OT - photosynthesis OT - post-translational modifications EDAT- 2017/11/11 06:00 MHDA- 2019/04/04 06:00 CRDT- 2017/11/11 06:00 PHST- 2016/05/23 00:00 [received] PHST- 2017/10/19 00:00 [accepted] PHST- 2017/11/11 06:00 [pubmed] PHST- 2019/04/04 06:00 [medline] PHST- 2017/11/11 06:00 [entrez] AID - 10.1111/pce.13100 [doi] PST - ppublish SO - Plant Cell Environ. 2018 May;41(5):1139-1153. doi: 10.1111/pce.13100. Epub 2018 Jan 9.