PMID- 29127884
OWN - NLM
STAT- MEDLINE
DCOM- 20180917
LR  - 20190201
IS  - 1873-6823 (Electronic)
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 66
DP  - 2018 Feb
TI  - Regional dysregulation of taurine and related amino acids in the fetal rat brain 
      following gestational alcohol exposure.
PG  - 27-33
LID - S0741-8329(17)30792-9 [pii]
LID - 10.1016/j.alcohol.2017.07.010 [doi]
AB  - The fetal brain exhibits exquisite alcohol-induced regional neuronal 
      vulnerability. A candidate mechanism for alcohol-mediated brain deficits is 
      disruption of amino acid (AA) bioavailability. AAs are vitally important for 
      proper neurodevelopment, as they comprise the most abundant neurotransmitters in 
      the brain and act as neurotransmitter precursors, nitric oxide donors, 
      antioxidants, and neurotrophic factors, which induce synaptogenesis, neuronal 
      proliferation, and migration. We hypothesized that gestational alcohol alters 
      brain AA concentrations, disrupts AAs associated with neuropathogenesis, and that 
      alterations are region-specific. We assigned pregnant Sprague-Dawley rats to 
      either a pair-fed control or a binge alcohol treatment group on gestational day 
      (GD) 4. Alcohol animals were acclimatized via a once-daily orogastric gavage of a 
      4.5 g/kg alcohol dose from GD 5-10, and progressed to a 6 g/kg alcohol dose from 
      GD 11-20. Pair-fed animals received isocaloric maltose dextrin (once daily; GD 
      5-20). Fetal cerebral cortex, cerebellum, and hippocampus were collected on GD 
      21. Following collection, Fluorometric High Performance Liquid Chromatography 
      (HPLC) involving pre-column derivatization with o-phthaldialdehyde quantified 
      regional content of 22 AAs. Chronic binge alcohol administration to pregnant dams 
      regionally altered AA concentrations in all three structures, with the cerebral 
      cortex exhibiting the least vulnerability and the hippocampus exhibiting maximal 
      vulnerability. We conjecture that the AA imbalances observed in this study are 
      critically implicated in pathological and compensatory processes occurring in the 
      brain in response to gestational alcohol exposure.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Lunde-Young, Raine
AU  - Lunde-Young R
AD  - Department of Veterinary Physiology and Pharmacology, College of Veterinary 
      Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 
      77843, USA.
FAU - Davis-Anderson, Katie
AU  - Davis-Anderson K
AD  - Department of Veterinary Physiology and Pharmacology, College of Veterinary 
      Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 
      77843, USA.
FAU - Naik, Vishal
AU  - Naik V
AD  - Department of Veterinary Physiology and Pharmacology, College of Veterinary 
      Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 
      77843, USA.
FAU - Nemec, Matthew
AU  - Nemec M
AD  - Department of Veterinary Physiology and Pharmacology, College of Veterinary 
      Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 
      77843, USA.
FAU - Wu, Guoyao
AU  - Wu G
AD  - Department of Animal Science, Texas A&M University, College Station, TX, 77843, 
      USA.
FAU - Ramadoss, Jayanth
AU  - Ramadoss J
AD  - Department of Veterinary Physiology and Pharmacology, College of Veterinary 
      Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 
      77843, USA. Electronic address: jramadoss@cvm.tamu.edu.
LA  - eng
GR  - K99 AA019446/AA/NIAAA NIH HHS/United States
GR  - R00 AA019446/AA/NIAAA NIH HHS/United States
GR  - R01 AA023520/AA/NIAAA NIH HHS/United States
GR  - R21 AA023035/AA/NIAAA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170930
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 1EQV5MLY3D (Taurine)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Brain/embryology/*metabolism/physiopathology
MH  - Cerebellum/embryology/metabolism/physiopathology
MH  - Cerebral Cortex/embryology/metabolism/physiopathology
MH  - Disease Models, Animal
MH  - *Ethanol
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/*metabolism/physiopathology
MH  - Fetus/*metabolism/physiopathology
MH  - Gestational Age
MH  - Hippocampus/embryology/metabolism/physiopathology
MH  - Maternal Exposure
MH  - Pregnancy
MH  - Rats, Sprague-Dawley
MH  - Taurine/*metabolism
PMC - PMC5743598
MID - NIHMS910079
OTO - NOTNLM
OT  - Alcohol
OT  - Gestation
OT  - Teratology
COIS- Conflict of interest: None.
EDAT- 2017/11/12 06:00
MHDA- 2018/09/18 06:00
PMCR- 2019/02/01
CRDT- 2017/11/12 06:00
PHST- 2017/05/17 00:00 [received]
PHST- 2017/07/21 00:00 [revised]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/11/12 06:00 [pubmed]
PHST- 2018/09/18 06:00 [medline]
PHST- 2017/11/12 06:00 [entrez]
PHST- 2019/02/01 00:00 [pmc-release]
AID - S0741-8329(17)30792-9 [pii]
AID - 10.1016/j.alcohol.2017.07.010 [doi]
PST - ppublish
SO  - Alcohol. 2018 Feb;66:27-33. doi: 10.1016/j.alcohol.2017.07.010. Epub 2017 Sep 30.