PMID- 29128675
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20190201
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 86
DP  - 2018 Feb
TI  - Regionally variant collagen alignment correlates with viscoelastic properties of 
      the disc of the human temporomandibular joint.
PG  - 1-6
LID - S0003-9969(17)30355-2 [pii]
LID - 10.1016/j.archoralbio.2017.11.002 [doi]
AB  - OBJECTIVE: To determine the regionally variant quality of collagen alignment in 
      human TMJ discs and its statistical correlation with viscoelastic properties. 
      DESIGN: For quantitative analysis of the quality of collagen alignment, 
      horizontal sections of human TMJ discs with Pricrosirius Red staining were imaged 
      under circularly polarized microscopy. Mean angle and angular deviation of 
      collagen fibers in each region were analyzed using a well-established automated 
      image-processing for angular gradient. Instantaneous and relaxation moduli of 
      each disc region were measured under stress-relaxation test both in tensile and 
      compression. Then Spearman correlation analysis was performed between the angular 
      deviation and the moduli. To understand the effect of glycosaminoglycans on the 
      correlation, TMJ disc samples were treated by chondroitinase ABC (C-ABC). 
      RESULTS: Our imaging processing analysis showed the region-variant direction of 
      collagen alignment, consistently with previous findings. Interestingly, the 
      quality of collagen alignment, not only the directions, was significantly 
      different in between the regions. The angular deviation of fiber alignment in the 
      anterior and intermediate regions were significantly smaller than the posterior 
      region. Medial and lateral regions showed significantly bigger angular deviation 
      than all the other regions. The regionally variant angular deviation values 
      showed statistically significant correlation with the tensile instantaneous 
      modulus and the relaxation modulus, partially dependent on C-ABC treatment. 
      CONCLUSION: Our findings suggest the region-variant degree of collagen fiber 
      alignment is likely attributed to the heterogeneous viscoelastic properties of 
      TMJ disc that may have significant implications in development of regenerative 
      therapy for TMJ disc.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Gutman, Shawn
AU  - Gutman S
AD  - Regenerative Engineering Laboratory, Section for Oral and Maxillofacial Surgery, 
      College of Dental Medicine, Columbia University, 630 W. 168 St. - VC12-230, New 
      York, NY 10032, United States.
FAU - Kim, Daniel
AU  - Kim D
AD  - Regenerative Engineering Laboratory, Section for Oral and Maxillofacial Surgery, 
      College of Dental Medicine, Columbia University, 630 W. 168 St. - VC12-230, New 
      York, NY 10032, United States.
FAU - Tarafder, Solaiman
AU  - Tarafder S
AD  - Regenerative Engineering Laboratory, Section for Oral and Maxillofacial Surgery, 
      College of Dental Medicine, Columbia University, 630 W. 168 St. - VC12-230, New 
      York, NY 10032, United States.
FAU - Velez, Sergio
AU  - Velez S
AD  - Regenerative Engineering Laboratory, Section for Oral and Maxillofacial Surgery, 
      College of Dental Medicine, Columbia University, 630 W. 168 St. - VC12-230, New 
      York, NY 10032, United States.
FAU - Jeong, Julia
AU  - Jeong J
AD  - Regenerative Engineering Laboratory, Section for Oral and Maxillofacial Surgery, 
      College of Dental Medicine, Columbia University, 630 W. 168 St. - VC12-230, New 
      York, NY 10032, United States.
FAU - Lee, Chang H
AU  - Lee CH
AD  - Regenerative Engineering Laboratory, Section for Oral and Maxillofacial Surgery, 
      College of Dental Medicine, Columbia University, 630 W. 168 St. - VC12-230, New 
      York, NY 10032, United States. Electronic address: chl2109@cumc.columbia.edu.
LA  - eng
GR  - R03 DE026794/DE/NIDCR NIH HHS/United States
PT  - Journal Article
DEP - 20171108
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Glycosaminoglycans)
RN  - 9007-34-5 (Collagen)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Adult
MH  - Algorithms
MH  - Chondroitin ABC Lyase/pharmacology
MH  - Collagen/metabolism/*physiology
MH  - Compressive Strength
MH  - Elastic Modulus
MH  - Female
MH  - Glycosaminoglycans/metabolism
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Male
MH  - Middle Aged
MH  - Staining and Labeling
MH  - Temporomandibular Joint/metabolism/*physiology
MH  - Tensile Strength
MH  - Viscosity
PMC - PMC5745264
MID - NIHMS919378
OTO - NOTNLM
OT  - Automated image processing
OT  - Collagen fiber alignment
OT  - TMJ disc
OT  - Viscoelastic properties
EDAT- 2017/11/13 06:00
MHDA- 2018/08/31 06:00
PMCR- 2019/02/01
CRDT- 2017/11/13 06:00
PHST- 2017/09/21 00:00 [received]
PHST- 2017/11/06 00:00 [revised]
PHST- 2017/11/07 00:00 [accepted]
PHST- 2017/11/13 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
PHST- 2017/11/13 06:00 [entrez]
PHST- 2019/02/01 00:00 [pmc-release]
AID - S0003-9969(17)30355-2 [pii]
AID - 10.1016/j.archoralbio.2017.11.002 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2018 Feb;86:1-6. doi: 10.1016/j.archoralbio.2017.11.002. Epub 
      2017 Nov 8.