PMID- 29128909
OWN - NLM
STAT- MEDLINE
DCOM- 20190422
LR  - 20190422
IS  - 1438-8359 (Electronic)
IS  - 0913-8668 (Linking)
VI  - 32
IP  - 1
DP  - 2018 Feb
TI  - Central noradrenergic activity affects analgesic effect of Neuropeptide S.
PG  - 48-53
LID - 10.1007/s00540-017-2427-y [doi]
AB  - BACKGROUND: Neuropeptide S (NPS) is an endogenous neuropeptide controlling 
      anxiolysis, wakefulness, and analgesia. NPS containing neurons exist near to the 
      locus coeruleus (LC) involved in the descending anti-nociceptive system. NPS 
      interacts with central noradrenergic neurons; thus brain noradrenergic signaling 
      may be involved in NPS-induced analgesia. We tested NPS analgesia in 
      noradrenergic neuron-lesioned rats using a selective LC noradrenergic neurotoxin, 
      N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4). METHODS: A total 66 male 
      Sprague-Dawley rats weighing 350-450 g were used. Analgesic effects of NPS were 
      evaluated using hot-plate and tail-flick test with or without DSP-4. The animal 
      allocated into 3 groups; hot-plate with NPS alone intracerebroventricular (icv) 
      (0.0, 1.0, 3.3, and 10.0 nmol), tail-flick NPS alone icv (0.0 and 10.0 nmol), and 
      hot-plate with NPS and DSP-4 (0 or 50 mg/kg ip). In hot-plate with NPS and DSP-4 
      group, noradrenaline content in the cerebral cortex, pons, hypothalamus, were 
      measured. RESULTS: NPS 10 nmol icv prolonged hot plate (%MPE) but not tail flick 
      latency at 30 and 40 min after administration. DSP-4 50 mg/kg decreased 
      noradrenaline content in the all 3 regions. The NA depletion inhibited NPS 
      analgesic effect in the hot plate test but not tail flick test. There was a 
      significant correlation between hot plate latency (percentage of maximum possible 
      effect: %MPE) with NPS 10 nmol and NA content in the cerebral cortex (p = 0.017, 
      r (2) = 0.346) which noradrenergic innervation arisen mainly from the LC. No 
      other regions had the correlation. CONCLUSIONS: NPS analgesia interacts with LC 
      noradrenergic neuronal activity.
FAU - Jinushi, Kei
AU  - Jinushi K
AD  - Department of Anesthesiology, Hirosaki University Hospital, Hirosaki, 036-8563, 
      Japan.
FAU - Kushikata, Tetsuya
AU  - Kushikata T
AUID- ORCID: 0000-0003-4263-0177
AD  - Department of Anesthesiology, Hirosaki University Graduate School of Medicine, 
      Zaifu 5, Hirosaki, 036-8562, Japan. tetsuyak@hirosaki-u.ac.jp.
FAU - Kudo, Takashi
AU  - Kudo T
AD  - Department of Anesthesiology, Hirosaki University Hospital, Hirosaki, 036-8563, 
      Japan.
FAU - Calo, Girolamo
AU  - Calo G
AD  - Section of Pharmacology, Department of Medical Science, National Institute of 
      Neuroscience, University of Ferrara, Ferrara, Italy.
FAU - Guerrini, Remo
AU  - Guerrini R
AD  - Department of Chemical and Pharmaceutical Sciences, LTTA, University of Ferrara, 
      44121, Ferrara, Italy.
FAU - Hirota, Kazuyoshi
AU  - Hirota K
AD  - Department of Anesthesiology, Hirosaki University Graduate School of Medicine, 
      Zaifu 5, Hirosaki, 036-8562, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171111
PL  - Japan
TA  - J Anesth
JT  - Journal of anesthesia
JID - 8905667
RN  - 0 (Analgesics)
RN  - 0 (Benzylamines)
RN  - PQ1P7JP5C1 (DSP 4)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Analgesics/*pharmacology
MH  - Animals
MH  - Benzylamines/*pharmacology
MH  - Brain/metabolism
MH  - Hypothalamus/metabolism
MH  - Locus Coeruleus/metabolism
MH  - Male
MH  - Neurons/metabolism
MH  - Norepinephrine/*metabolism
MH  - Pain/*drug therapy
MH  - Pain Measurement
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Analgesia
OT  - Central noradrenergic neuron
OT  - DSP-4
OT  - Hotplate
OT  - Neuropeptide S
EDAT- 2017/11/13 06:00
MHDA- 2019/04/23 06:00
CRDT- 2017/11/13 06:00
PHST- 2017/04/06 00:00 [received]
PHST- 2017/11/02 00:00 [accepted]
PHST- 2017/11/13 06:00 [pubmed]
PHST- 2019/04/23 06:00 [medline]
PHST- 2017/11/13 06:00 [entrez]
AID - 10.1007/s00540-017-2427-y [pii]
AID - 10.1007/s00540-017-2427-y [doi]
PST - ppublish
SO  - J Anesth. 2018 Feb;32(1):48-53. doi: 10.1007/s00540-017-2427-y. Epub 2017 Nov 11.