PMID- 29129923
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20181113
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 8
IP  - 1
DP  - 2017 Nov 13
TI  - Ist1 regulates ESCRT-III assembly and function during multivesicular endosome 
      biogenesis in Caenorhabditis elegans embryos.
PG  - 1439
LID - 10.1038/s41467-017-01636-8 [doi]
LID - 1439
AB  - Degradation of most integral membrane proteins is directed by the endosomal 
      sorting complex required for transport (ESCRT) machinery, which selectively 
      targets ubiquitin-modified cargoes into intralumenal vesicles (ILVs) within 
      multivesicular endosomes (MVEs). To better understand the mechanisms underlying 
      ESCRT-mediated formation of ILVs, we exploited the rapid, de novo biogenesis of 
      MVEs during the oocyte-to-embryo transition in C. elegans. In contrast to 
      previous models suggesting that ILVs form individually, we demonstrate that they 
      remain tethered to one another subsequent to internalization, arguing that they 
      bud continuously from stable subdomains. In addition, we show that membrane 
      bending and ILV formation are directed specifically by the ESCRT-III complex in 
      vivo in a manner regulated by Ist1, which promotes ESCRT-III assembly and 
      inhibits the incorporation of upstream ESCRT components into ILVs. Our findings 
      underscore essential actions for ESCRT-III in membrane remodeling, cargo 
      selection, and cargo retention, which act repetitively to maximize the rate of 
      ILV formation.
FAU - Frankel, E B
AU  - Frankel EB
AD  - Department of Biomolecular Chemistry, University of Wisconsin-Madison School of 
      Medicine and Public Health, 440 Henry Mall, Madison, WI, 53706, USA.
FAU - Shankar, Raakhee
AU  - Shankar R
AD  - Department of Biomolecular Chemistry, University of Wisconsin-Madison School of 
      Medicine and Public Health, 440 Henry Mall, Madison, WI, 53706, USA.
FAU - Moresco, James J
AU  - Moresco JJ
AD  - The Scripps Research Institute, 10550 North Torrey Pines Rd., Department of 
      Chemical Physiology, La Jolla, CA, 92037, USA.
FAU - Yates, John R 3rd
AU  - Yates JR 3rd
AUID- ORCID: 0000-0001-5267-1672
AD  - The Scripps Research Institute, 10550 North Torrey Pines Rd., Department of 
      Chemical Physiology, La Jolla, CA, 92037, USA.
FAU - Volkmann, Niels
AU  - Volkmann N
AD  - Bioinformatics and Structural Biology Program, Sanford-Burnham Medical Research 
      Institute, 10901N Torrey Pines Rd, La Jolla, CA, 92037, USA.
FAU - Audhya, Anjon
AU  - Audhya A
AUID- ORCID: 0000-0002-7828-5152
AD  - Department of Biomolecular Chemistry, University of Wisconsin-Madison School of 
      Medicine and Public Health, 440 Henry Mall, Madison, WI, 53706, USA. 
      audhya@wisc.edu.
LA  - eng
GR  - P41 GM103533/GM/NIGMS NIH HHS/United States
GR  - R01 GM115972/GM/NIGMS NIH HHS/United States
GR  - T32 GM007215/GM/NIGMS NIH HHS/United States
GR  - R01 GM088151/GM/NIGMS NIH HHS/United States
GR  - R01 GM110567/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20171113
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (IST-1 protein, C elegans)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Vesicular Transport Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Animals
MH  - Caenorhabditis elegans/*embryology
MH  - Caenorhabditis elegans Proteins/genetics/*metabolism
MH  - Cell Membrane/*metabolism
MH  - Endosomal Sorting Complexes Required for 
      Transport/biosynthesis/genetics/*metabolism
MH  - Multivesicular Bodies/*metabolism
MH  - Oocytes/growth & development
MH  - Protein Transport/physiology
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Vesicular Transport Proteins/*metabolism
PMC - PMC5682282
COIS- The authors declare no competing financial interests.
EDAT- 2017/11/14 06:00
MHDA- 2018/09/12 06:00
PMCR- 2017/11/13
CRDT- 2017/11/14 06:00
PHST- 2017/03/23 00:00 [received]
PHST- 2017/10/03 00:00 [accepted]
PHST- 2017/11/14 06:00 [entrez]
PHST- 2017/11/14 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
PHST- 2017/11/13 00:00 [pmc-release]
AID - 10.1038/s41467-017-01636-8 [pii]
AID - 1636 [pii]
AID - 10.1038/s41467-017-01636-8 [doi]
PST - epublish
SO  - Nat Commun. 2017 Nov 13;8(1):1439. doi: 10.1038/s41467-017-01636-8.