PMID- 29130646
OWN - NLM
STAT- MEDLINE
DCOM- 20180705
LR  - 20180713
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Linking)
VI  - 69
IP  - 12
DP  - 2017 Dec
TI  - Investigating the expression of MMPs and TIMPs in preterm placenta and role of 
      CpG methylation in regulating MMP-9 expression.
PG  - 985-993
LID - 10.1002/iub.1687 [doi]
AB  - Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) are 
      crucial to the processes of normal labor and parturition. We have previously 
      reported aberrant protein levels of MMPs in placenta of women delivering preterm 
      as compared to term. In this study, we examine the mRNA levels of MMPs (MMP-1, 
      MMP-2, and MMP-9) and TIMPs (TIMP-1, TIMP-2) in the placenta from women 
      delivering preterm as compared with term and further study the promoter DNA 
      methylation of the MMP-9 gene in a sub-sample of term and preterm placentae. A 
      total of 110 women were included in the study; 56 delivered term and 54 delivered 
      preterm. MMP and TIMP mRNA levels were determined by Taqman-based qPCR. Promoter 
      CpG methylation of MMP-9 gene was studied on a subset of 10 term and 8 preterm 
      placenta using Epitect Methyl-II PCR assay kit. The mRNA levels of MMP-1,-2 were 
      higher and those of TIMP-1,-2 were lower in the placentae of women delivering 
      preterm. MMP-9 levels were comparable between the two groups. Among women 
      undergoing spontaneous vaginal deliveries, higher mRNA levels of MMP-1, -2 and -9 
      were seen in the placentae of those delivering preterm as compared to term. 
      Similar results were seen in women undergoing preterm labor as compared to term. 
      MMP-9 gene promoter was hypomethylated in preterm placenta as compared to term 
      placenta, while the mRNA levels were comparable between the two groups. The 
      observed imbalance between MMP and TIMP expression may have prematurely triggered 
      the signaling cascade leading to preterm birth. (c) 2017 IUBMB Life, 
      69(12):985-993, 2017.
CI  - (c) 2017 International Union of Biochemistry and Molecular Biology.
FAU - Sundrani, Deepali
AU  - Sundrani D
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
FAU - Narang, Ankita
AU  - Narang A
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
FAU - Mehendale, Savita
AU  - Mehendale S
AD  - Department of Obstetrics and Gynecology, Bharati Hospital and Research Center, 
      Pune, Maharashtra, India.
FAU - Joshi, Sadhana
AU  - Joshi S
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
FAU - Chavan-Gautam, Preeti
AU  - Chavan-Gautam P
AUID- ORCID: 0000-0001-8514-3171
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171111
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (RNA, Messenger)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (TIMP2 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - *CpG Islands
MH  - DNA Methylation
MH  - *Epigenesis, Genetic
MH  - Female
MH  - Humans
MH  - Infant, Newborn
MH  - Infant, Premature
MH  - Matrix Metalloproteinase 1/genetics/metabolism
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/*genetics/metabolism
MH  - Parturition/physiology
MH  - Placenta/*metabolism/pathology
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction
MH  - Tissue Inhibitor of Metalloproteinase-1/*genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/*genetics/metabolism
OTO - NOTNLM
OT  - epigenetics
OT  - extra cellular matrix
OT  - gene regulation
OT  - placenta
OT  - pregnancy
EDAT- 2017/11/14 06:00
MHDA- 2018/07/06 06:00
CRDT- 2017/11/14 06:00
PHST- 2017/07/26 00:00 [received]
PHST- 2017/09/26 00:00 [accepted]
PHST- 2017/11/14 06:00 [pubmed]
PHST- 2018/07/06 06:00 [medline]
PHST- 2017/11/14 06:00 [entrez]
AID - 10.1002/iub.1687 [doi]
PST - ppublish
SO  - IUBMB Life. 2017 Dec;69(12):985-993. doi: 10.1002/iub.1687. Epub 2017 Nov 11.