PMID- 29130662
OWN - NLM
STAT- MEDLINE
DCOM- 20190530
LR  - 20190530
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 48
IP  - 5
DP  - 2017 Sep
TI  - [The Role of Bone Morphogenic Protein-4 in the Progression of Vein Graft 
      Hyperplastic Remodeling under Hyperglycemic Condition].
PG  - 710-715
AB  - OBJECTIVE: This study was designed to assess the vessel wall characteristics and 
      the expression levels of bone morphogenic protein4(BMP4) and proliferating cell 
      antigen Ki67 in vein grafts harvested from diabetic rats,and to investigate the 
      role of BMP4 in progression of vein graft hyperplastic remodeling under 
      hyperglycemic condition. METHODS: 48 male SpragueDawky rats [body mass (194+/-16) 
      g] aged 8 weeks were randomly divided into diabetes mellitus (DM) group ( n=24) 
      and nondiabetes mellitus (NDM) group ( n=24). The DM rats were induced by 
      streptozotocin in combination with highsugar and highfat diet. The unilateral 
      external jugular vein was interposition grafted into the common carotid arteries 
      in the two groups. The vein grafts were harvested at preoperatively and 1,2 and 4 
      weeks postoperatively ( n=6) in each group. The morphological characteristics of 
      the venous graft wall were evaluated by hematoxylineosin staining,and the 
      expression levels and the distribution of Ki67 and BMP4 were assessed by 
      immunohistochemistry analysis,then the expression of BMP4 gene and protein was 
      measured by realtime polymerase chain reaction (RTPCR) and Western blot assay 
      respectively. RESULTS: In the progression of rats vein grafts hyperplastic 
      remodeling,the venous wall thickness of rats thickened with time increasing,and 
      the intimal and medial thickness of the vein grafts harvested from DM rats were 
      significantly higher than those from NDM rats at the same time postoperatively ( 
      P<0.05). Ki-67 was highly xpressed in smooth muscle cells nucleus located in the 
      rats vein grafts,and its expression level was up-regulated gradually in the 
      progression of vein graft failure,and the Ki 67 positive cells of vein grafts 
      from DM rats were significantly higher than those from NDM controls at the same 
      period ( P<0.05). Immunohistochemistry analysis showed that BMP4 was expressed in 
      smooth muscle cells cytoplasmof the rats vein grafts,combined with the results of 
      RT-PCR and Western blot assay,there was a little BMP4 expression could be seen in 
      venous wall of NDM rats,while BMP4 positive cells and the expression level of 
      BMP4 gene and protein from DM rats vein grafts was increasing with obvious time 
      dependence and significantly higher than the NDM rats ( P<0.05). CONCLUSION: The 
      morphological and pathological changes within the venous wall were significantly 
      correlated with the high expression levels of BMP4 in vein grafts harvested from 
      diabetic rats,implying a potential role of BMP4 in the progression of accelerated 
      vein graft failure under hyperglycemic condition.
FAU - Fang, Deng-Feng
AU  - Fang DF
AD  - Department of Anesthesiology,West China Hospital,Sichuan University,Chengdu 
      610041,China.
FAU - Zhang, Hong-Wei
AU  - Zhang HW
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
FAU - Gu, Jun
AU  - Gu J
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
FAU - Fan, Kang-Jun
AU  - Fan KJ
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
FAU - Liang, Huai-Min
AU  - Liang HM
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
FAU - Shen, Jia-Yu
AU  - Shen JY
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
FAU - Zhang, Er-Yong
AU  - Zhang EY
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
FAU - Hu, Jia
AU  - Hu J
AD  - Department of Cardiovascular Surgery,West China Hospital,Sichuan 
      University,Chengdu 610041,China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Bmp4 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 4/*metabolism
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Hyperplasia
MH  - Jugular Veins/*transplantation
MH  - Ki-67 Antigen/metabolism
MH  - Male
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Vascular Remodeling
OTO - NOTNLM
OT  - Bone morphogenic protein-4
OT  - Coronary artery bypass graft
OT  - Hyperglycemic condition
OT  - Vein grafts
EDAT- 2017/11/14 06:00
MHDA- 2019/05/31 06:00
CRDT- 2017/11/14 06:00
PHST- 2017/11/14 06:00 [entrez]
PHST- 2017/11/14 06:00 [pubmed]
PHST- 2019/05/31 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Sep;48(5):710-715.