PMID- 29130972
OWN - NLM
STAT- MEDLINE
DCOM- 20171229
LR  - 20171229
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 174
IP  - 3-4
DP  - 2017
TI  - Human Dendritic Cells with Th2-Polarizing Capacity: Analysis Using Label-Free 
      Quantitative Proteomics.
PG  - 170-182
LID - 10.1159/000479512 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the sentinels of the immune system. Upon 
      recognition of a pathogen, they mature and migrate to draining lymph nodes to 
      prime and polarize T cell responses. Although it is known that helminths and 
      helminth-derived molecules condition DCs to polarize T helper (Th) cells towards 
      Th2, the underlying mechanisms remain incompletely understood. OBJECTIVES: The 
      aim of this study was to conduct a proteome analysis of helminth 
      antigen-stimulated DCs in order to gain more insight into the cellular processes 
      associated with their ability to polarize immune responses. METHODS: We analyzed 
      the maturation and polarization of monocyte-derived DCs from 9 donors at 2 
      different time points after stimulation with different Th1- and Th2-polarizing 
      pathogen-derived molecules. The samples were measured using liquid 
      chromatography-Fourier transform ion cyclotron resonance mass spectrometry for 
      relative quantitation. RESULTS: Lipopolysaccharide-induced maturation promoted 
      the expression of proteins related to metabolic, cellular, and immune system 
      processes. Th1-polarizing DCs, conditioned by IFN-gamma during maturation, displayed 
      accelerated maturation by differentially expressing cytoskeletal proteins and 
      proteins involved in immune regulation. The stimulation of DCs with soluble egg 
      antigens and omega-1 derived from Schistosoma mansoni, which are both 
      Th2-inducing stimuli, increased 60S acidic ribosomal protein P2, and vesicle 
      amine transferase 1 while decreasing the expression of proteins related to 
      antigen processing and presentation. CONCLUSION: Our data indicate that not only 
      proteins involved in the interaction between T cells and DCs at the level of the 
      immunological synapse, but also those related to cellular metabolism and stress, 
      may promote Th2 polarization.
CI  - (c) 2017 S. Karger AG, Basel.
FAU - Hussaarts, Leonie
AU  - Hussaarts L
AD  - Department of Parasitology, Leiden University Medical Center (LUMC), Leiden, The 
      Netherlands.
FAU - Kaisar, Maria Mardalena Martini
AU  - Kaisar MMM
FAU - Guler, Arzu Tugce
AU  - Guler AT
FAU - Dalebout, Hans
AU  - Dalebout H
FAU - Everts, Bart
AU  - Everts B
FAU - Deelder, Andre M
AU  - Deelder AM
FAU - Palmblad, Magnus
AU  - Palmblad M
FAU - Yazdanbakhsh, Maria
AU  - Yazdanbakhsh M
LA  - eng
PT  - Journal Article
DEP - 20171110
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Antigens, Helminth)
RN  - 0 (Egg Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (omega-1 antigen, Schistosoma mansoni)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Helminth/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Egg Proteins/immunology
MH  - Humans
MH  - Immune Evasion
MH  - Interferon-gamma/immunology
MH  - Lipopolysaccharides/immunology
MH  - Proteomics
MH  - Schistosoma mansoni/*immunology
MH  - Schistosomiasis mansoni/*immunology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
OTO - NOTNLM
OT  - Dendritic cells
OT  - Helminths
OT  - Omega-1
OT  - Proteome
OT  - T helper 2 responses
EDAT- 2017/11/14 06:00
MHDA- 2017/12/30 06:00
CRDT- 2017/11/14 06:00
PHST- 2017/01/19 00:00 [received]
PHST- 2017/07/11 00:00 [accepted]
PHST- 2017/11/14 06:00 [pubmed]
PHST- 2017/12/30 06:00 [medline]
PHST- 2017/11/14 06:00 [entrez]
AID - 000479512 [pii]
AID - 10.1159/000479512 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2017;174(3-4):170-182. doi: 10.1159/000479512. Epub 
      2017 Nov 10.