PMID- 29132106
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20181015
IS  - 1095-7103 (Electronic)
IS  - 0021-9797 (Linking)
VI  - 513
DP  - 2018 Mar 1
TI  - Silver oxide nanoparticles embedded silk fibroin spuns: Microwave mediated 
      preparation, characterization and their synergistic wound healing and 
      anti-bacterial activity.
PG  - 62-71
LID - S0021-9797(17)31287-0 [pii]
LID - 10.1016/j.jcis.2017.11.001 [doi]
AB  - The synergistic wound healing and antibacterial activity of silver oxide 
      nanoparticles embedded silk fibroin (Ag(2)O-SF) spuns is reported here. UV-Vis 
      spectro photometric analysis of these spuns showed the surface plasmon resonance 
      (SPR) confirming the formation of the silver oxide nanoparticles (Ag(2)O NPs) on 
      the surface of the silk fibroin (SF). Scanning electron microscope (SEM) and 
      Differential scanning calorimetry (DSC) also confirmed the presence of Ag(2)O NPs 
      on surface of SF. X-ray diffraction (XRD) analysis revealed the crystalline 
      nature of both SF and Ag(2)O-SF. Fourier transform infrared spectroscopy (FT-IR) 
      results showed the different forms of silk (I and II) and their corresponding 
      protein (amide I, II, III) confirmations. Biodegradation study revealed 
      insignificant changes in the morphology of Ag(2)O-SF spuns even after 14 days of 
      immersion in phosphate buffered saline (PBS). Ag(2)O-SF spuns showed excellent 
      antibacterial activity against both pathogen (S. aureus and M. tuberculosis) and 
      non-pathogen (E. coli) bacteria. More importantly, In vitro wound healing 
      (scratch assay) assay revealed fast migration of the T3T fibroblast cells through 
      the scratch area treated with extract of Ag(2)O-SF spuns and the area was 
      completely covered within 24 h. Cytotoxicity assay confirmed the biocompatible 
      nature of the Ag(2)O-SF spuns, thus suggesting an ideal material for wound 
      healing and anti-bacterial applications.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Babu, Punuri Jayasekhar
AU  - Babu PJ
AD  - Bio Engineering and Drug Design Laboratory, Department of Biotechnology, Bhupat 
      and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, 
      Chennai 600 036, Tamil Nadu, India; Biomaterials Laboratory, Department of 
      Materials Engineering, Indian Institute of Science, Bangalore, Karnataka 560012, 
      India.
FAU - Doble, Mukesh
AU  - Doble M
AD  - Bio Engineering and Drug Design Laboratory, Department of Biotechnology, Bhupat 
      and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, 
      Chennai 600 036, Tamil Nadu, India. Electronic address: mukeshd@iitm.ac.in.
FAU - Raichur, Ashok M
AU  - Raichur AM
AD  - Biomaterials Laboratory, Department of Materials Engineering, Indian Institute of 
      Science, Bangalore, Karnataka 560012, India.
LA  - eng
PT  - Journal Article
DEP - 20171102
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Oxides)
RN  - 0 (Silver Compounds)
RN  - 897WUN6G6T (disilver oxide)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/chemistry/*pharmacology
MH  - Bacteria/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Fibroins/*chemistry
MH  - Metal Nanoparticles/*chemistry
MH  - Mice
MH  - *Microwaves
MH  - NIH 3T3 Cells
MH  - Oxides/chemistry/*pharmacology
MH  - Silver Compounds/chemistry/*pharmacology
MH  - Wound Healing/*drug effects
OTO - NOTNLM
OT  - Antibacterial activity
OT  - Biocompatibility
OT  - Silk fibroin spuns
OT  - Silver oxide nanoparticles
OT  - Wound healing activity
EDAT- 2017/11/14 06:00
MHDA- 2018/10/16 06:00
CRDT- 2017/11/14 06:00
PHST- 2017/10/03 00:00 [received]
PHST- 2017/10/31 00:00 [revised]
PHST- 2017/11/01 00:00 [accepted]
PHST- 2017/11/14 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2017/11/14 06:00 [entrez]
AID - S0021-9797(17)31287-0 [pii]
AID - 10.1016/j.jcis.2017.11.001 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2018 Mar 1;513:62-71. doi: 10.1016/j.jcis.2017.11.001. 
      Epub 2017 Nov 2.