PMID- 29132256
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1477-0393 (Electronic)
IS  - 0748-2337 (Linking)
VI  - 34
IP  - 1
DP  - 2018 Jan
TI  - MCP-1 produced by keratinocytes is associated with leucocyte recruitment during 
      elicitation of nickel-induced occupational allergic contact dermatitis.
PG  - 36-43
LID - 10.1177/0748233717738633 [doi]
AB  - To investigate the expression profile of monocyte chemoattractant peptide-1 
      (MCP-1) by keratinocytes after nickel exposure and to identify its role for 
      leucocyte migration during nickel-induced occupational allergic contact 
      dermatitis (OACD), 26 workers diagnosed with nickel-induced OACD were enrolled. 
      Skin biopsies from the positive nickel-challenged sites at different time points 
      were assessed by immunohistochemistry (IHC) for MCP-1, CD68, CD45RO, and in situ 
      hybridization (ISH) for MCP-1, using chronic periumbilical dermititis as 
      controls. The expressions of MCP-1 in HaCaT cell culture after nickel treatment 
      were quantified by enzyme-linked immunosorbent assay. The results showed that at 
      positive nickel-challenged sites, strong expressions of MCP-1, both messenger RNA 
      (mRNA) and protein, were detected in the basal keratinocytes during the early 
      phase (24-48 h after nickel application), paralleled by the recruitment of 
      CD68(+) and CD45RO(+) cells to the skin compartments. The expressions of MCP-1 
      declined gradually in the late phase (72-96 h after nickel application). 
      Treatment with nickel sulfate at noncytotoxic concentrations (0.01-100 microM) 
      induced a concentration-related elevation of MCP-1 expression by HaCaT cells 
      compared to the untreated cells. The data indicated that a temporal expression 
      pattern of MCP-1 produced by keratinocytes after nickel exposure was involved in 
      the complex process of mononuclear cell infiltration during elicitation of 
      nickel-induced OACD. Targeting MCP-1 might be a potential therapeutic strategy 
      for OACD.
FAU - Mai, Weihua
AU  - Mai W
AD  - 1 Department of Preventive Medicine, The Fifth Affiliated Hospital of Sun Yat-Sen 
      University, Zhuhai, Guangdong, China.
FAU - Lu, Dongqing
AU  - Lu D
AD  - 2 Department of Dermatology, The Fifth Affiliated Hospital of Sun Yat-Sen 
      University, Zhuhai, Guangdong, China.
FAU - Liu, Xingwei
AU  - Liu X
AD  - 3 Department of General Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen 
      University, Zhuhai, Guangdong, China.
FAU - Chen, Ling
AU  - Chen L
AD  - 4 Department of Medical Quality Control, The Fifth Affiliated Hospital of Sun 
      Yat-Sen University, Zhuhai, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20171113
PL  - England
TA  - Toxicol Ind Health
JT  - Toxicology and industrial health
JID - 8602702
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 7OV03QG267 (Nickel)
SB  - IM
MH  - Adult
MH  - Cell Line, Transformed
MH  - Chemokine CCL2/analysis/*immunology/metabolism
MH  - Chemotaxis, Leukocyte/*immunology
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Female
MH  - Humans
MH  - Keratinocytes/*immunology/metabolism
MH  - Leukocytes/immunology
MH  - Male
MH  - Nickel/*adverse effects
MH  - Occupational Diseases/*immunology
MH  - Skin/chemistry/drug effects/pathology
OTO - NOTNLM
OT  - HaCaT keratinocyte
OT  - Monocyte chemoattractant peptide-1
OT  - leucocyte migration
OT  - nickel
OT  - occupational allergic contact dermatitis
EDAT- 2017/11/15 06:00
MHDA- 2018/12/12 06:00
CRDT- 2017/11/15 06:00
PHST- 2017/11/15 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/11/15 06:00 [entrez]
AID - 10.1177/0748233717738633 [doi]
PST - ppublish
SO  - Toxicol Ind Health. 2018 Jan;34(1):36-43. doi: 10.1177/0748233717738633. Epub 
      2017 Nov 13.