PMID- 29133088
OWN - NLM
STAT- MEDLINE
DCOM- 20180809
LR  - 20180809
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 109
DP  - 2018 Apr 1
TI  - Fructose-human serum albumin interaction undergoes numerous biophysical and 
      biochemical changes before forming AGEs and aggregates.
PG  - 896-906
LID - S0141-8130(17)33517-1 [pii]
LID - 10.1016/j.ijbiomac.2017.11.069 [doi]
AB  - Fructose is a reducing and highly lipogenic sugar that has unique metabolic 
      effects in the liver. Non-enzymatic fructosylation of proteins generates advanced 
      glycation end products (AGEs). Human serum albumin (HSA) may undergo 
      fructosylation vis-a-vis AGEs formation. High fructose consumption may lead to 
      structurally altered and functionally compromised fructosylated-HSA-AGEs, which 
      can cause damage to hepatocytes resulting in hepatic macro- and microvesicular 
      steatosis. In this study, HSA was incubated with varying concentrations of 
      fructose for 10days and the induced changes were studied. Fructosylated-HSA 
      exhibited hyperchromicity, increased AGE-specific fluorescence, quenching of 
      tryptophan fluorescence and increased melting temperature. 
      Nepsilon-[carboxymethyl]-lysine (CML), was detected by liquid chromatography mass 
      spectrometry (LC-MS). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis 
      (SDS-PAGE) results showed decreased mobility in fructosylated-HSA. Perturbations 
      in secondary and tertiary structure were revealed by fourier transform-infrared 
      spectroscopy (FT-IR), supported by far- and near-UV circular dichroism (CD). 
      Dynamic light scattering (DLS) and Matrix-Assisted Laser Desorption 
      Ionization-Time of Flight (MALDI-TOF) mass spectrometry studies suggested 
      increase in molecular mass of fructosylated-HSA. Amyloidogenic aggregates were 
      confirmed from Congo red, Thioflavin T assay and Scanning electron microscope 
      (SEM). These investigations confirmed the structural alterations in 
      fructosylated-HSA and warrants further study to probe the role of 
      fructosylated-HSA-AGEs in hepatopathy vis-a-vis fatty liver diseases.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Zaman, Asif
AU  - Zaman A
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, 
      Aligarh, India.
FAU - Arif, Zarina
AU  - Arif Z
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, 
      Aligarh, India.
FAU - Moinuddin
AU  - Moinuddin
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, 
      Aligarh, India.
FAU - Alam, Khursheed
AU  - Alam K
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, 
      Aligarh, India. Electronic address: kalam786@rediffmail.com.
LA  - eng
PT  - Journal Article
DEP - 20171111
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Protein Aggregates)
RN  - 30237-26-4 (Fructose)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Chromatography, Liquid
MH  - Circular Dichroism
MH  - Dynamic Light Scattering
MH  - Fructose/*chemistry/*metabolism
MH  - Glycation End Products, Advanced/*chemistry/*metabolism
MH  - Humans
MH  - Mass Spectrometry
MH  - *Protein Aggregates
MH  - Serum Albumin, Human/*chemistry/*metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - AGEs
OT  - Fructose
OT  - Human serum albumin
EDAT- 2017/11/15 06:00
MHDA- 2018/08/10 06:00
CRDT- 2017/11/15 06:00
PHST- 2017/09/12 00:00 [received]
PHST- 2017/11/08 00:00 [revised]
PHST- 2017/11/10 00:00 [accepted]
PHST- 2017/11/15 06:00 [pubmed]
PHST- 2018/08/10 06:00 [medline]
PHST- 2017/11/15 06:00 [entrez]
AID - S0141-8130(17)33517-1 [pii]
AID - 10.1016/j.ijbiomac.2017.11.069 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2018 Apr 1;109:896-906. doi: 10.1016/j.ijbiomac.2017.11.069. 
      Epub 2017 Nov 11.