PMID- 29135090
OWN - NLM
STAT- MEDLINE
DCOM- 20190814
LR  - 20190814
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 22
IP  - 5
DP  - 2017 Sep-Oct
TI  - Gene expression profiling demonstrates WNT/beta-catenin pathway genes alteration in 
      Mexican patients with colorectal cancer and diabetes mellitus.
PG  - 1107-1114
AB  - PURPOSE: Several studies have shown a strong association between diabetes 
      mellitus (DM) and increased risk of colorectal cancer (CRC). The fundamental 
      mechanisms that support this association are not entirely understood; however, it 
      is believed that hyperinsulinemia and hyperglycemia may be involved. Some 
      proposed mechanisms include upregulation of mitogenic signaling pathways like 
      MAPK, PI3K, mTOR, and WNT, which are involved in cell proliferation, growth, and 
      cancer cell survival. The purpose of this study was to evaluate the gene 
      expression profile and identify differently expressed genes involved in mitogenic 
      pathways in CRC patients with and without DM. METHODS: In this study, microarray 
      analysis of gene expression followed by quantitative PCR (qPCR) was performed in 
      cancer tissue from CRC patients with and without DM to identify the gene 
      expression profiles and validate the differently expressed genes. RESULTS: Among 
      the study groups, some differently expressed genes were identified. However, when 
      bioinformatics clustering tools were used, a significant modulation of genes 
      involved in the WNT pathway was evident. Therefore, we focused on genes 
      participating in this pathway, such as WNT3A, LRP6, TCF7L2, and FRA-1. Validation 
      of the expression levels of those genes by qPCR showed that CRC patients without 
      type 2 diabetes mellitus (T2DM) expressed significantly more WNT3Ay LRP6, but 
      less TCF7L2 and FRA-1 compared to controls, while in CRC patients with DM the 
      expression levels of WNT3A, LRP6, TCF7L2, and FRA-1 were significantly higher 
      compared to controls. CONCLUSIONS: Our results suggest that WNT/beta-catenin pathway 
      is upregulated in patients with CRC and DM, demonstrating its importance and 
      involvement in both pathologies.
FAU - Ivonne Wence-Chavez, Laura
AU  - Ivonne Wence-Chavez L
AD  - Doctorate Program in Molecular Biology, Health Sciences University Center, 
      University of Guadalajara, Guadalajara, Jalisco, Mexico.
FAU - Palomares-Chacon, Ulises
AU  - Palomares-Chacon U
FAU - Pablo Flores-Gutierrez, Juan
AU  - Pablo Flores-Gutierrez J
FAU - Felipe Jave-Suarez, Luis
AU  - Felipe Jave-Suarez L
FAU - Del Carmen Aguilar-Lemarroy, Adriana
AU  - Del Carmen Aguilar-Lemarroy A
FAU - Barros-Nunez, Patricio
AU  - Barros-Nunez P
FAU - Esperanza Flores-Martinez, Silvia
AU  - Esperanza Flores-Martinez S
FAU - Sanchez-Corona, Jose
AU  - Sanchez-Corona J
FAU - Alejandra Rosales-Reynoso, Monica
AU  - Alejandra Rosales-Reynoso M
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (beta Catenin)
MH  - Aged
MH  - Colorectal Neoplasms/*genetics/pathology
MH  - Diabetes Mellitus, Type 2/*genetics/pathology
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Humans
MH  - Male
MH  - Mexico
MH  - Middle Aged
MH  - Wnt Signaling Pathway/*physiology
MH  - beta Catenin/*genetics
EDAT- 2017/11/15 06:00
MHDA- 2019/08/15 06:00
CRDT- 2017/11/15 06:00
PHST- 2017/11/15 06:00 [entrez]
PHST- 2017/11/15 06:00 [pubmed]
PHST- 2019/08/15 06:00 [medline]
PST - ppublish
SO  - J BUON. 2017 Sep-Oct;22(5):1107-1114.