PMID- 29135113
OWN - NLM
STAT- MEDLINE
DCOM- 20190731
LR  - 20190731
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 22
IP  - 5
DP  - 2017 Sep-Oct
TI  - Minichromosome maintenance (MCM) protein 4 overexpression is a potential 
      prognostic marker for laryngeal squamous cell carcinoma.
PG  - 1272-1277
AB  - PURPOSE: The minichromosomal maintenance (MCM) proteins are involved in the 
      initiation and DNA replication. The role of MCM4 remains to be elucidated. The 
      purpose of this study was to investigate the effects of MCM4 in laryngeal 
      squamous cell carcinoma (LSCC) cell growth and apoptosis. METHODS: LSCC cell line 
      UMSCC 5 was used in this study. The small interfering RNA (siRNA) of MCM 4 gene 
      was used to identify the effects of MCM4 on the proliferation and apoptosis using 
      methylimidazole tetrazolium (MTT) assay and flow-cytometry, respectively. 
      Confirmed LSCC and adjacent non-tumor tissues were collected from 34 patients who 
      were willing to participate in the study, from 2010 through 2015, from 163 
      patients undergoing treatment in the Department of Otorhinolaryngology/Head and 
      Neck Surgery of Beijing Tongren Hospital in Capital Medical University of P.R. 
      China. Immunohistochemical staining of MCM4 expression in the resected tissues 
      was performed to analyze the correlation between its expression and the 
      clinicopathological characteristics. RESULTS: The results showed that siRNA of 
      MCM4 could significantly inhibit LSCC cell line UMSCC 5 proliferation and induce 
      apoptosis. MCM4 mRNA was higher expressed in carcinoma tissues than in adjacent 
      normal tissues. MCM4 expression was correlated with male gender, smoking history 
      and poor differentiation. CONCLUSIONS: We noticed a significant role for MCM4 
      overexpression in human LSCC tissues and their corresponding adjacent 
      non-neoplastic tissues and found that siRNA of MCM4 can significantly decrease 
      the proliferation of cancer cells. It is suggested that MCM4 profiling could 
      potentially be used to predict response to treatment and prognosis in LSCC.
FAU - Han, Jianfeng
AU  - Han J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing, 100730, China.
FAU - Lian, Meng
AU  - Lian M
FAU - Fang, Jugao
AU  - Fang J
FAU - Liu, Honggang
AU  - Liu H
FAU - Wang, Ru
AU  - Wang R
FAU - Zhai, Jie
AU  - Zhai J
FAU - Fan Yang, Yi
AU  - Fan Yang Y
FAU - Fen, Ling
AU  - Fen L
FAU - Shi, Qian
AU  - Shi Q
FAU - Zhi Ma, Hong
AU  - Zhi Ma H
FAU - Sun, Juan
AU  - Sun J
FAU - Ma, Zhihong
AU  - Ma Z
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (MCMBP protein, human)
RN  - 0 (Nuclear Proteins)
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Proliferation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Nuclear Proteins/*metabolism
MH  - Prognosis
EDAT- 2017/11/15 06:00
MHDA- 2019/08/01 06:00
CRDT- 2017/11/15 06:00
PHST- 2017/11/15 06:00 [entrez]
PHST- 2017/11/15 06:00 [pubmed]
PHST- 2019/08/01 06:00 [medline]
PST - ppublish
SO  - J BUON. 2017 Sep-Oct;22(5):1272-1277.