PMID- 29136690
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20201209
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 46
IP  - 11
DP  - 2017 Nov 8
TI  - [Values of JAZF1 gene rearrangement detected by fluorescence in-situ 
      hybridization in diagnosis of endometrial stromal tumours].
PG  - 769-774
LID - 10.3760/cma.j.issn.0529-5807.2017.11.007 [doi]
AB  - Objective: To investigate the role of JAZF1 gene rearrangement in the diagnosis 
      and differential diagnosis of endometrial stromal sarcomas by fluorescence in 
      situ hybridization (FISH). Methods: JAZF1 gene rearrangement was analyzed by FISH 
      in 129 cases of ESS diagnosed from January 2008 to December 2016 including 105 
      cases of low-grade endometrial stromal sarcoma (LG-ESS), 21 cases of high-grade 
      endometrial stromal sarcoma (HG-ESS) and 3 cases of undifferentiated uterine 
      sarcoma (UUS). Sixteen cases of the related tumours in uterus were also collected 
      as control group. The results were compared with our previous studies of 
      JAZF1/JJAZ1 fusion gene in ESS by RT-PCR. Results: Detection of JAZF1 gene 
      rearrangement by FISH was successfully analyzed in 144 cases. JAZF1 gene 
      alteration was detected in 63 cases, all of which were LG-ESS, with an overall 
      positivity of 60.6% (63/104), while no JAZF1 gene rearrangement was found in all 
      other cases. JAZF1 gene rearrangement was present in LG-ESS with classic 
      histology (69.3%, 52/75), smooth muscle differentiation (2/10), sex cord-like 
      differentiation (4/5), fibromyxoid change (1/5), clear cell change (0/1), 
      skeletal muscle differentiation (0/1), and schwannoma-like palisading pattern 
      (0/1). The different components in all the cases of LG-ESS with variant histology 
      had the clonal origin, with or without JAZF1 gene alteration. Compared to the 
      results of JAZF1/JJAZ1 fusion gene by RT-PCR, the positive rate of JAZF1 gene 
      rearrangement in LG-ESS by FISH (61.9%, 26/42) was significantly higher than that 
      of RT-PCR (30.0%, 12/40; P<0.01). Conclusions: JAZF1 gene rearrangement is 
      present only in LG-ESS, but not in HG-ESS, UUS or other related tumours in 
      uterus. The frequency of JAZF1 gene rearrangement varies between classic LG-ESS 
      and different morphologic variants. It is frequently, but not consistently, 
      present in classic LG-ESS and less often positive in variant cases. The results 
      of JAZF1 gene alterations in LG-ESS with different morphologic variants support 
      the contention that the endometrial stromal and their variant morphologic 
      components have the same clonal origin. Detection of JAZF1 gene rearrangement by 
      FISH is very useful for the diagnosis and differential diagnosis of ESS.
FAU - Bai, Q M
AU  - Bai QM
AD  - Department of Pathology, Fudan University Shanghai Cancer Center, Department of 
      Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
FAU - Chang, B
AU  - Chang B
FAU - Tu, X Y
AU  - Tu XY
FAU - Bi, R
AU  - Bi R
FAU - Cheng, Y F
AU  - Cheng YF
FAU - Huang, D
AU  - Huang D
FAU - Zhu, X L
AU  - Zhu XL
FAU - Wu, L J
AU  - Wu LJ
FAU - Zhang, X
AU  - Zhang X
FAU - Zhou, X Y
AU  - Zhou XY
FAU - Yang, W T
AU  - Yang WT
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (JAZF1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Co-Repressor Proteins
MH  - DNA-Binding Proteins
MH  - Endometrial Neoplasms/*diagnosis/*genetics
MH  - Endometrial Stromal Tumors/*diagnosis/*genetics
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Proteins/*genetics
MH  - Sarcoma, Endometrial Stromal/*diagnosis/*genetics
MH  - Transcription Factors
OTO - NOTNLM
OT  - In situ hybridization, fluorescence
OT  - Reverse transcriptase polymerase chain reaction
OT  - Sarcoma, endometrial stromal
OT  - Translocation, genetic
EDAT- 2017/11/15 06:00
MHDA- 2017/12/27 06:00
CRDT- 2017/11/15 06:00
PHST- 2017/11/15 06:00 [entrez]
PHST- 2017/11/15 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-5807.2017.11.007 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2017 Nov 8;46(11):769-774. doi: 
      10.3760/cma.j.issn.0529-5807.2017.11.007.