PMID- 29138532
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20190116
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2017
DP  - 2017
TI  - Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited 
      Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells.
PG  - 6909415
LID - 10.1155/2017/6909415 [doi]
LID - 6909415
AB  - We investigated the regulatory roles of USP2 in mRNA accumulation of 
      proinflammatory cytokines in macrophage-like cells after stimulation with a 
      toll-like receptor (TLR) 4 ligand, lipopolysaccharide (LPS). Human 
      macrophage-like HL-60 cells, mouse macrophage-like J774.1 cells, and mouse 
      peritoneal macrophages demonstrated negative feedback to USP2 mRNA levels after 
      LPS stimulation, suggesting that USP2 plays a significant role in LPS-stimulated 
      macrophages. USP2 knockdown (KD) by short hairpin RNA in HL-60 cells promoted the 
      accumulation of transcripts for 25 of 104 cytokines after LPS stimulation. In 
      contrast, limited induction of cytokines was observed in cells forcibly 
      expressing the longer splice variant of USP2 (USP2A), or in peritoneal 
      macrophages isolated from Usp2a transgenic mice. An ubiquitin 
      isopeptidase-deficient USP2A mutant failed to suppress LPS-induced cytokine 
      expression, suggesting that protein ubiquitination contributes to USP2-mediated 
      cytokine repression. Although USP2 deficiency did not accelerate TNF 
      receptor-associated factor (TRAF) 6-nuclear factor-kappaB (NF-kappaB) signaling, it 
      increased the DNA binding ratio of the octamer binding transcription factor 
      (Oct)-1 to Oct-2 in TNF, CXCL8, CCL4, and IL6 promoters. USP2 decreased nuclear 
      Oct-2 protein levels in addition to decreasing the polyubiquitination of Oct-1. 
      In summary, USP2 modulates proinflammatory cytokine induction, possibly through 
      modification of Oct proteins, in macrophages following TLR4 activation.
FAU - Kitamura, Hiroshi
AU  - Kitamura H
AUID- ORCID: 0000-0001-9643-3211
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, School of 
      Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, 
      Hokkaido 069-8501, Japan.
AD  - Department of Comparative and Experimental Medicine, Graduate School of Medical 
      Sciences, Nagoya City University, Nagoya, Aichi, Japan.
FAU - Ishino, Takeshi
AU  - Ishino T
AUID- ORCID: 0000-0001-6714-4242
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, School of 
      Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, 
      Hokkaido 069-8501, Japan.
FAU - Shimamoto, Yoshinori
AU  - Shimamoto Y
AUID- ORCID: 0000-0002-4212-498X
AD  - Laboratory of Animal Therapeutics, Department of Veterinary Science, Rakuno 
      Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
FAU - Okabe, Jun
AU  - Okabe J
AUID- ORCID: 0000-0002-7196-0522
AD  - Department of Diabetes, Central Clinical School, Faculty of Medicine, Nursing and 
      Health Sciences, Monash University, 99 Commercial Road, Melbourne, VIC 3004, 
      Australia.
FAU - Miyamoto, Tomomi
AU  - Miyamoto T
AD  - Department of Comparative and Experimental Medicine, Graduate School of Medical 
      Sciences, Nagoya City University, Nagoya, Aichi, Japan.
FAU - Takahashi, Eiki
AU  - Takahashi E
AD  - Research Resources Center, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, 
      Saitama, Japan.
FAU - Miyoshi, Ichiro
AU  - Miyoshi I
AUID- ORCID: 0000-0001-6578-3390
AD  - Department of Comparative and Experimental Medicine, Graduate School of Medical 
      Sciences, Nagoya City University, Nagoya, Aichi, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170912
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Cytokines)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.19.12 (USP2 protein, Drosophila)
RN  - EC 3.4.19.12 (Ubiquitin-Specific Proteases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Drosophila Proteins/*metabolism
MH  - HL-60 Cells
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 4/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Ubiquitin-Specific Proteases/*metabolism
PMC - PMC5613470
EDAT- 2017/11/16 06:00
MHDA- 2018/07/03 06:00
PMCR- 2017/09/12
CRDT- 2017/11/16 06:00
PHST- 2017/06/02 00:00 [received]
PHST- 2017/07/21 00:00 [revised]
PHST- 2017/07/30 00:00 [accepted]
PHST- 2017/11/16 06:00 [entrez]
PHST- 2017/11/16 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
PHST- 2017/09/12 00:00 [pmc-release]
AID - 10.1155/2017/6909415 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2017;2017:6909415. doi: 10.1155/2017/6909415. Epub 2017 Sep 
      12.