PMID- 29138860
OWN - NLM
STAT- MEDLINE
DCOM- 20180712
LR  - 20180712
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 1
DP  - 2018 Jan
TI  - An RNA‑sequencing study identifies candidate genes for angiotensin II‑induced 
      cardiac remodeling.
PG  - 1954-1962
LID - 10.3892/mmr.2017.8043 [doi]
AB  - The present study aimed to reveal the underlying mechanism of angiotensin II 
      (AngII)‑induced cardiac remodeling and to identify potential therapeutic targets 
      for prevention. Rat cardiac fibroblasts (CFs) were cultured with 10 nM AngII for 
      12 h, and CFs without AngII were used as the control. Following RNA isolation 
      from AngII treated and control CFs, RNA‑sequencing was performed to detect gene 
      expression levels. Differentially‑expressed genes (DEGs) were identified using 
      the linear models for microarray analysis package in R software, and their 
      functions and pathways were examined via enrichment analysis. In addition, 
      potential associations at the protein level were revealed via the construction of 
      a protein‑protein interaction (PPI) network. The expression levels of genes of 
      interest were validated via reverse transcription‑quantitative polymerase chain 
      reaction analysis. In total, 126 upregulated and 140 downregulated DEGs were 
      identified. According to the enrichment analysis, acetyl coA carboxylase beta 
      (ACACB), interleukin 1beta (IL1B), interleukin 1alpha (IL1A), nitric oxide synthase 2 
      (NOS2) and matrix metallopeptidase 3 (MMP3) were associated with the immune 
      response, regulation of angiogenesis, superoxide metabolic process and carboxylic 
      acid binding biological processes. Among them, ACACB and MPP3 were two 
      predominant nodes in the PPI network. In addition, IL1B and MMP3 were 
      demonstrated to be upregulated. These five genes, particularly IL1B and MMP3, may 
      be used as candidate markers for the prevention of AngII‑induced cardiac 
      remodeling.
FAU - Shu, Jin
AU  - Shu J
AD  - Department of Gerontology, Tongren Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200336, P.R. China.
FAU - Liu, Zhanwen
AU  - Liu Z
AD  - Department of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai 200336, P.R. China.
FAU - Jin, Li
AU  - Jin L
AD  - Department of Gerontology, Tongren Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai 200336, P.R. China.
FAU - Wang, Haiya
AU  - Wang H
AD  - Department of Gerontology, Renji Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Shanghai 200001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171114
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/*physiology
MH  - Animals
MH  - Rats
MH  - Sequence Analysis, RNA
MH  - *Transcriptome
MH  - *Ventricular Remodeling
EDAT- 2017/11/16 06:00
MHDA- 2018/07/13 06:00
CRDT- 2017/11/16 06:00
PHST- 2017/03/13 00:00 [received]
PHST- 2017/08/24 00:00 [accepted]
PHST- 2017/11/16 06:00 [pubmed]
PHST- 2018/07/13 06:00 [medline]
PHST- 2017/11/16 06:00 [entrez]
AID - 10.3892/mmr.2017.8043 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Jan;17(1):1954-1962. doi: 10.3892/mmr.2017.8043. Epub 2017 Nov 
      14.