PMID- 29139241 OWN - NLM STAT- MEDLINE DCOM- 20180911 LR - 20180911 IS - 1863-2378 (Electronic) IS - 1863-1959 (Linking) VI - 65 IP - 1 DP - 2018 Feb TI - Porcine respiratory disease complex after the introduction of H1N1/2009 influenza virus in Brazil. PG - e155-e161 LID - 10.1111/zph.12424 [doi] AB - From 2009 to 2015, 74 lungs from suckling (6.8%), nursing (70.3%), fattening (20.3%) pigs and pregnant sows (2.7%) with respiratory signs from pig farms in Southern Brazil were submitted to a diagnostic laboratory for necropsy and/or histologic examination and screening for respiratory agents by RT-qPCR, immunohistochemistry (IHC), virus isolation (VI) and subtyping for influenza A virus (IAV), IHC and nested PCR for Mycoplasma hyopneumoniae (Mhyo), PCR for porcine circovirus 2 (PCV2), RT-qPCR for porcine reproductive and respiratory syndrome virus (PRRSV) and bacterial culture. All lung samples were positive for IAV using RT-qPCR. Seventy-two lungs had histologic lesions associated with acute to subacute IAV infection characterized by necrotizing bronchiolitis/bronchitis or bronchointerstitial pneumonia with lymphocytic peribronchiolitis and bronchiolar/bronchial hyperplasia, respectively. Forty-nine lungs (66.2%) were positive by IHC for IAV nucleoprotein. The H1N1/2009 was the most common subtype and the only IAV detected in 58.1% of lungs, followed by H1N2 (9.5%) and H3N2 (6.8%). Coinfection of IAV and Mhyo was seen in 23 (31%) cases. Although 14.9% of the lungs were positive for PCV2 using PCR, no suggestive lesions of PCV2 disease were observed. Porcine reproductive and respiratory syndrome virus (PRRSV) was not detected, consistent with the PRRS-free status of Brazil. Secondary bacterial infections (8/38) were associated with suppurative bronchopneumonia and/or pleuritis. Primary IAV infection with Mhyo coinfection was the most common agents found in porcine respiratory disease complex (PRDC) in pigs in Southern Brazil. CI - (c) 2017 Blackwell Verlag GmbH. FAU - Rech, R R AU - Rech RR AD - Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA. FAU - Gava, D AU - Gava D AD - Embrapa Suinos e Aves, Concordia, SC, Brazil. FAU - Silva, M C AU - Silva MC AD - Qualem Laboratorio Veterinario, Santa Maria, RS, Brazil. FAU - Fernandes, L T AU - Fernandes LT AD - Laboratorio Cedisa, Concordia, SC, Brazil. FAU - Haach, V AU - Haach V AD - Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. FAU - Ciacci-Zanella, J R AU - Ciacci-Zanella JR AD - Embrapa Suinos e Aves, Concordia, SC, Brazil. FAU - Schaefer, R AU - Schaefer R AUID- ORCID: 0000-0002-3511-0221 AD - Embrapa Suinos e Aves, Concordia, SC, Brazil. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171114 PL - Germany TA - Zoonoses Public Health JT - Zoonoses and public health JID - 101300786 SB - IM MH - Animals MH - Brazil/epidemiology MH - Circovirus/isolation & purification MH - *Influenza A Virus, H1N1 Subtype MH - Lung/microbiology/pathology/virology MH - Mycoplasma hyopneumoniae/isolation & purification MH - Orthomyxoviridae Infections/epidemiology/*veterinary/virology MH - Porcine respiratory and reproductive syndrome virus/isolation & purification MH - Respiratory Tract Diseases/epidemiology/*veterinary/virology MH - Swine MH - Swine Diseases/epidemiology/*virology OTO - NOTNLM OT - Mycoplasma hyopneumoniae OT - influenza A virus OT - pneumonia OT - swine EDAT- 2017/11/16 06:00 MHDA- 2018/09/12 06:00 CRDT- 2017/11/16 06:00 PHST- 2017/06/19 00:00 [received] PHST- 2017/11/16 06:00 [pubmed] PHST- 2018/09/12 06:00 [medline] PHST- 2017/11/16 06:00 [entrez] AID - 10.1111/zph.12424 [doi] PST - ppublish SO - Zoonoses Public Health. 2018 Feb;65(1):e155-e161. doi: 10.1111/zph.12424. Epub 2017 Nov 14.