PMID- 29140992
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 11
DP  - 2017
TI  - Transcriptome profile of lung dendritic cells after in vitro porcine reproductive 
      and respiratory syndrome virus (PRRSV) infection.
PG  - e0187735
LID - 10.1371/journal.pone.0187735 [doi]
LID - e0187735
AB  - The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease 
      that leads to high financial and production losses in the global swine industry. 
      The pathogenesis of this disease is dependent on a multitude of factors, and its 
      control remains problematic. The immune system generally defends against 
      infectious diseases, especially dendritic cells (DCs), which play a crucial role 
      in the activation of the immune response after viral infections. However, the 
      understanding of the immune response and the genetic impact on the immune 
      response to PRRS virus (PRRSV) remains incomplete. In light of this, we 
      investigated the regulation of the host immune response to PRRSV in porcine lung 
      DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds 
      (Pietrain and Duroc) were collected before (0 hours) and during various periods 
      of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis 
      revealed a total of 20,396 predicted porcine genes, which included breed-specific 
      differentially expressed immune genes. Pietrain and Duroc infected lung DCs 
      showed opposite gene expression courses during the first time points post 
      infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung 
      DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis 
      revealed time-dependent co-expressed groups of genes that were involved in 
      immune-relevant pathways. Key clusters and pathways were identified, which help 
      to explain the biological and functional background of lung DCs post PRRSV 
      infection and suggest IL-1beta1 as an important candidate gene. RNA-Seq was also 
      used to characterize the viral replication of PRRSV for each breed. PRRSV was 
      able to infect and to replicate differently in lung DCs between the two mentioned 
      breeds. These results could be useful in investigations on immunity traits in pig 
      breeding and enhancing the health of pigs.
FAU - Proll, Maren Julia
AU  - Proll MJ
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Neuhoff, Christiane
AU  - Neuhoff C
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Schellander, Karl
AU  - Schellander K
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Uddin, Muhammad Jasim
AU  - Uddin MJ
AD  - School of Veterinary Science, The University of Queensland, Gatton Campus, 
      Gatton, Australia.
FAU - Cinar, Mehmet Ulas
AU  - Cinar MU
AD  - Department of Animal Science, Faculty of Agriculture, Erciyes University, 
      Kayseri, Turkey.
FAU - Sahadevan, Sudeep
AU  - Sahadevan S
AD  - BRIC - Biotech Research & Innovation Centre, University of Copenhagen, 
      Copenhagen, Denmark.
FAU - Qu, Xueqi
AU  - Qu X
AD  - Laboratory for Gene and Cell Engineering, Institute of Biomedicine and 
      Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of 
      Sciences, Shenzhen, China.
FAU - Islam, Md Aminul
AU  - Islam MA
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Poirier, Mikhael
AU  - Poirier M
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Muller, Marcel A
AU  - Muller MA
AD  - Institute of Virology, Helmut-Ruska-Haus, Charite Medical School, Berlin, 
      Germany.
FAU - Drosten, Christian
AU  - Drosten C
AD  - Institute of Virology, Helmut-Ruska-Haus, Charite Medical School, Berlin, 
      Germany.
FAU - Tesfaye, Dawit
AU  - Tesfaye D
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Tholen, Ernst
AU  - Tholen E
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
FAU - Grosse-Brinkhaus, Christine
AU  - Grosse-Brinkhaus C
AD  - Institute of Animal Science, Department of Animal Breeding and Husbandry, 
      University of Bonn, Bonn, Germany.
LA  - eng
PT  - Journal Article
DEP - 20171115
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Dendritic Cells/*metabolism/pathology
MH  - Female
MH  - Gene Expression Profiling
MH  - Host-Pathogen Interactions
MH  - Multigene Family
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Swine
MH  - *Transcriptome
PMC - PMC5687707
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/11/16 06:00
MHDA- 2017/12/12 06:00
PMCR- 2017/11/15
CRDT- 2017/11/16 06:00
PHST- 2016/07/19 00:00 [received]
PHST- 2017/10/25 00:00 [accepted]
PHST- 2017/11/16 06:00 [entrez]
PHST- 2017/11/16 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PHST- 2017/11/15 00:00 [pmc-release]
AID - PONE-D-16-28854 [pii]
AID - 10.1371/journal.pone.0187735 [doi]
PST - epublish
SO  - PLoS One. 2017 Nov 15;12(11):e0187735. doi: 10.1371/journal.pone.0187735. 
      eCollection 2017.