PMID- 29142434
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220310
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 13
IP  - Suppl 3
DP  - 2017 Oct
TI  - Melastoma malabathricum Ethyl Acetate Fraction Induces Secondary Necrosis in 
      Human Breast and Lung Cancer Cell Lines.
PG  - S688-S692
LID - 10.4103/pm.pm_465_15 [doi]
AB  - BACKGROUND: Melastoma malabathricum (MM) is a traditional plant used in the 
      Borneo region. The cytotoxic effects of methanol extracts from MM leaves have 
      been reported in a number of human cancer cell lines. However, the mode of cell 
      death by MM has not been investigated. OBJECTIVE: We investigated the cytotoxic 
      effects of MM in both human breast and lung cancer cell lines, MCF-7 and A549, 
      respectively, and defined the mode of cell death. MATERIALS AND METHODS: Cell 
      viability was measured using the 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl 
      tetrazolium bromide (MTT) assay. Annexin-V/propidium iodide (PI) and terminal 
      deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was done to 
      determine the mode of cell death. RESULTS: The MTT assay revealed that MM extract 
      had an IC50 of >400 mug/ml on both cell lines at 24 h posttreatment. Flow 
      cytometric and fluorescence microscopy analysis of Annexin-V/PI stained 
      MM-treated cells revealed that the majority of the cells underwent secondary 
      necrosis/late apoptosis. TUNEL assay showed that little to no DNA nicks were 
      present in MM-treated cells, suggesting that cells have undergone secondary 
      necrosis, not late apoptosis, at that time point. CONCLUSION: MCF-7 and A549 
      cells undergoes secondary necrosis 24 h post-treatment with MM extract. MM leaf 
      extract could be a potential source for a novel anti-tumor agent for cancer 
      therapy. SUMMARY: Melastoma malabathricum (MM) extract was toxic on human breast 
      and lung cancer cell linesMajority of MM-treated cells died by either secondary 
      necrosis or late apoptosis at 24 h post-treatmentTerminal deoxynucleotidyl 
      transferase dUTP nick-end labeling assay confirmed that MM-treated cells 
      underwent secondary necrosis, not late apoptosis. Abbreviations used: DMSO: 
      Dimethyl sulfoxide; MM: Melastoma malabathricum; MTT: 3-(4-, 
      5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; PI: Propidium iodide; 
      TUNEL: Terminal deoxynucleotidyl transferase dUTP nick-end labeling.
FAU - Idris, Adi
AU  - Idris A
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
FAU - Zulkipli, Ihsan N
AU  - Zulkipli IN
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
FAU - Zulhilmi, Nurul Ramizah
AU  - Zulhilmi NR
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
FAU - Lee, Huan F
AU  - Lee HF
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
FAU - Rajabalaya, Rajan
AU  - Rajabalaya R
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
FAU - Chee, Lim Y
AU  - Chee LY
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
FAU - Majid, Mohamed
AU  - Majid M
AD  - Faculty of Sciences, Universiti, Gadong, Brunei Darussalam.
FAU - David, Sheba R
AU  - David SR
AD  - PAP Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti, Gadong, 
      Brunei Darussalam.
LA  - eng
PT  - Journal Article
DEP - 20171011
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC5669117
OTO - NOTNLM
OT  - Apoptosis
OT  - Melastoma malabathricum
OT  - cancer
OT  - cytotoxic
OT  - flow cytometry
OT  - necrosis
COIS- There are no conflicts of interest.
EDAT- 2017/11/17 06:00
MHDA- 2017/11/17 06:01
PMCR- 2017/10/01
CRDT- 2017/11/17 06:00
PHST- 2015/10/28 00:00 [received]
PHST- 2016/01/21 00:00 [revised]
PHST- 2017/11/17 06:00 [entrez]
PHST- 2017/11/17 06:00 [pubmed]
PHST- 2017/11/17 06:01 [medline]
PHST- 2017/10/01 00:00 [pmc-release]
AID - PM-13-688 [pii]
AID - 10.4103/pm.pm_465_15 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2017 Oct;13(Suppl 3):S688-S692. doi: 10.4103/pm.pm_465_15. Epub 
      2017 Oct 11.