PMID- 29142471
OWN - NLM
STAT- MEDLINE
DCOM- 20180706
LR  - 20181113
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 23
IP  - 40
DP  - 2017 Oct 28
TI  - Dachaihu decoction ameliorates pancreatic fibrosis by inhibiting macrophage 
      infiltration in chronic pancreatitis.
PG  - 7242-7252
LID - 10.3748/wjg.v23.i40.7242 [doi]
AB  - AIM: To explore the role of macrophages in chronic pancreatitis (CP) and the 
      effect of Dachaihu decoction (DCHD) on pancreatic fibrosis in mice. METHODS: 
      KunMing mice were randomly divided into a control group, CP group, and DCHD 
      group. In the CP and DCHD groups, mice were intraperitoneally injected with 20% 
      L-arginine (3 g/kg twice 1 d/wk for 6 wk). Mice in the DCHD group were 
      administered DCHD intragastrically at a dose of 14 g/kg/d 1 wk after CP 
      induction. At 2 wk, 4 wk and 6 wk post-modeling, the morphology of the pancreas 
      was observed using hematoxylin and eosin, and Masson staining. Interleukin-6 
      (IL-6) serum levels were assayed using an enzyme-linked immunosorbent assay. 
      Double immunofluorescence staining was performed to observe the co-expression of 
      F4/80 and IL-6 in the pancreas. Inflammatory factors including monocyte 
      chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha) 
      and IL-6 were determined using real time-polymerase chain reaction. Western blot 
      analysis was used to detect fibronectin levels in the pancreas. RESULTS: Compared 
      with the control group, mice with 20% L-arginine-induced CP had obvious 
      macrophage infiltration and a higher level of fibrosis. IL-6 serum concentrations 
      were significantly increased. Double immunofluorescence staining showed that IL-6 
      and F4/80 were co-expressed in the pancreas. With the administration of DCHD, the 
      infiltration of macrophages and degree of fibrosis in the pancreas were 
      significantly attenuated; IL-6, MCP-1 and MIP-1alpha mRNA, and fibronectin levels 
      were reduced. CONCLUSION: The dominant role of macrophages in the development of 
      CP was mainly related to IL-6 production. DCHD was effective in ameliorating 
      pancreatic fibrosis by inhibiting macrophage infiltration and inflammatory factor 
      secretion in the pancreas.
FAU - Duan, Li-Fang
AU  - Duan LF
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Xu, Xiao-Fan
AU  - Xu XF
AD  - Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Zhu, Lin-Jia
AU  - Zhu LJ
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Liu, Fang
AU  - Liu F
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Zhang, Xiao-Qin
AU  - Zhang XQ
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Wu, Nan
AU  - Wu N
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Fan, Jian-Wei
AU  - Fan JW
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Xin, Jia-Qi
AU  - Xin JQ
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China.
FAU - Zhang, Hong
AU  - Zhang H
AD  - Department of Pathophysiology, Shaanxi University of Chinese Medicine, Xianyang 
      712046, Shaanxi Province, China. zhangh1227@163.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccl3 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Fibronectins)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (interleukin-6, mouse)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Arginine/toxicity
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL3/metabolism
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibronectins/metabolism
MH  - Fibrosis
MH  - Humans
MH  - Interleukin-6/blood/metabolism
MH  - Macrophages/*drug effects/immunology/metabolism
MH  - Medicine, Chinese Traditional/methods
MH  - Mice
MH  - Pancreas/drug effects/*pathology
MH  - Pancreatitis, Chronic/blood/chemically induced/*drug therapy/immunology
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC5677205
OTO - NOTNLM
OT  - Dachaihu decoction
OT  - Interleukin-6
OT  - Macrophages
OT  - Pancreatic fibrosis
COIS- Conflict-of-interest statement: The authors declare that there are no conflicts 
      of interest related to this study.
EDAT- 2017/11/17 06:00
MHDA- 2018/07/07 06:00
PMCR- 2017/10/28
CRDT- 2017/11/17 06:00
PHST- 2017/07/31 00:00 [received]
PHST- 2017/09/22 00:00 [revised]
PHST- 2017/09/29 00:00 [accepted]
PHST- 2017/11/17 06:00 [entrez]
PHST- 2017/11/17 06:00 [pubmed]
PHST- 2018/07/07 06:00 [medline]
PHST- 2017/10/28 00:00 [pmc-release]
AID - 10.3748/wjg.v23.i40.7242 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2017 Oct 28;23(40):7242-7252. doi: 
      10.3748/wjg.v23.i40.7242.