PMID- 29142506
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220310
IS  - 1476-9255 (Print)
IS  - 1476-9255 (Electronic)
IS  - 1476-9255 (Linking)
VI  - 14
DP  - 2017
TI  - Detection of circulating natural antibodies to inflammatory cytokines in type-2 
      diabetes and clinical significance.
PG  - 24
LID - 10.1186/s12950-017-0171-6 [doi]
LID - 24
AB  - BACKGROUND: Inflammatory cytokines have been demonstrated to be involved in 
      developing insulin resistance and type-2 diabetes (T2D). Natural antibodies in 
      the circulation have protective effects on common diseases in humans. The present 
      study was thus designed to test the hypothesis that natural antibodies against 
      inflammatory cytokines could be associated with T2D. METHODS: An enzyme-linked 
      immunosorbent assay (ELISA) was developed in-house to detect plasma IgG against 
      peptide antigens derived from interleukin 1alpha (IL1alpha), IL1beta, IL6, IL8 and tumor 
      necrosis factor-alpha (TNF-alpha) in 200 patients with T2D and 220 control subjects. 
      RESULTS: Binary regression showed that compared with control subjects, T2D 
      patients had a decreased level of plasma anti-IL6 IgG (adjusted r(2)=0.034, 
      p=0.0001), anti-IL8 IgG (adjusted r(2)=0.021, p=0.002) and anti-TNF-alpha IgG 
      (adjusted r(2)=0.017, p=0.003). Female patients mainly contributed to decreased 
      levels of anti-IL6 IgG (adjusted r(2)=0.065, p=0.0008) and anti-IL8 IgG (adjusted 
      r(2)=0.056, p=0.003), while male patients mainly contributed to decreased 
      anti-TNF-alpha IgG levels (adjusted r(2)=0.024, p=0.005). ROC curve analysis revealed 
      a sensitivity of 16.5% against specificity of 95.5% for anti-IL6 IgG assay and a 
      sensitivity of 19.5% against specificity of 95.9% for anti-IL8 IgG assay. 
      Glycated hemoglobin levels measured after 6-month glucose-lowering treatment 
      appeared to be inversely correlated with plasma anti-IL1alpha IgG (r=-0.477, df=17, 
      p=0.039) and anti-IL6 IgG (r=-0.519, df=17, p=0.023) although such correlation 
      failed to survive the Bonferroni correction. CONCLUSIONS: Deficiency of natural 
      IgG against inflammatory cytokines is likely to be a risk factor for T2D 
      development and detection of such antibodies may be useful for personalized 
      treatment of the disease.
FAU - Cai, Weiyi
AU  - Cai W
AD  - Laboratory for Nursing Science & Institute of Laboratory Medicine, Guangdong 
      Medical University, No.1 Xincheng Road, Dongguan, 523808 China. ISNI: 0000 0004 
      1760 3078. GRID: grid.410560.6
FAU - Qiu, Cailing
AU  - Qiu C
AD  - Dalang Hospital of Dongguan, Dongguan, 523000 China.
FAU - Zhang, Hongyu
AU  - Zhang H
AD  - The Second Hospital, Jilin University, Changchun, 130041 China. ISNI: 0000 0004 
      1760 5735. GRID: grid.64924.3d
FAU - Chen, Xiangyun
AU  - Chen X
AD  - Dalang Hospital of Dongguan, Dongguan, 523000 China.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - The Second Hospital, Jilin University, Changchun, 130041 China. ISNI: 0000 0004 
      1760 5735. GRID: grid.64924.3d
FAU - Meng, Qingyong
AU  - Meng Q
AD  - Laboratory for Nursing Science & Institute of Laboratory Medicine, Guangdong 
      Medical University, No.1 Xincheng Road, Dongguan, 523808 China. ISNI: 0000 0004 
      1760 3078. GRID: grid.410560.6
FAU - Wei, Jun
AU  - Wei J
AD  - Division of Health Research, University of the Highlands & Islands, Centre for 
      Health Science, Perth Road, Inverness, IV2 3JH UK. ISNI: 0000 0001 2189 1357. 
      GRID: grid.23378.3d
LA  - eng
PT  - Journal Article
DEP - 20171106
PL  - England
TA  - J Inflamm (Lond)
JT  - Journal of inflammation (London, England)
JID - 101232234
PMC - PMC5674864
OTO - NOTNLM
OT  - ELISA
OT  - IgG antibody
OT  - Inflammatory cytokines
OT  - Natural antibodies
OT  - Type-2 diabetes
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: This work was approved by the 
      Institutional Review Board of the Second Hospital of Jilin University, Changchun, 
      China, (IRB#: SHJU2017-101), and performed in accordance with the ethical 
      standards as laid down in the 1964 Declaration of Helsinki and its later 
      amendments. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: All 
      authors declared that they have no competing interests. PUBLISHER'S NOTE: 
      Springer Nature remains neutral with regard to jurisdictional claims in published 
      maps and institutional affiliations.
EDAT- 2017/11/17 06:00
MHDA- 2017/11/17 06:01
PMCR- 2017/11/06
CRDT- 2017/11/17 06:00
PHST- 2017/07/26 00:00 [received]
PHST- 2017/10/28 00:00 [accepted]
PHST- 2017/11/17 06:00 [entrez]
PHST- 2017/11/17 06:00 [pubmed]
PHST- 2017/11/17 06:01 [medline]
PHST- 2017/11/06 00:00 [pmc-release]
AID - 171 [pii]
AID - 10.1186/s12950-017-0171-6 [doi]
PST - epublish
SO  - J Inflamm (Lond). 2017 Nov 6;14:24. doi: 10.1186/s12950-017-0171-6. eCollection 
      2017.