PMID- 29143761
OWN - NLM
STAT- MEDLINE
DCOM- 20181123
LR  - 20181123
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 9
IP  - 11
DP  - 2017 Oct 28
TI  - Antibody Cross-Reactivity between Porcine Cytomegalovirus (PCMV) and Human 
      Herpesvirus-6 (HHV-6).
LID - 10.3390/v9110317 [doi]
LID - 317
AB  - Porcine cytomegalovirus (PCMV) infection is widely prevalent among pigs, and PCMV 
      is one of the viruses which may be transmitted during xenotransplantation using 
      pig cells, tissues, or organs. While human cytomegalovirus (HCMV) is a major risk 
      factor for allotransplantation, it is still unclear whether PCMV is able to 
      infect human cells or pose a risk for xenotransplantation. Previously, it was 
      shown that transmission of PCMV after pig kidney to non-human primate 
      transplantations resulted in a significantly reduced survival time of the 
      transplanted organ. To detect PCMV, PCR-based and immunological methods were 
      used. Screening of pigs by Western blot analyses using recombinant viral proteins 
      revealed up to 100% of the tested animals to be infected. When the same method 
      was applied to screen human sera for PCMV-reactive antibodies, positive Western 
      blot results were obtained in butchers and workers in the meat industry as well 
      as in normal blood donors. To exclude an infection of humans with PCMV, the sera 
      were further investigated. PCMV is closely related to human herpesvirus-6 (HHV-6) 
      and human herpesvirus-7 (HHV-7), and a sequence alignment of glycoprotein B 
      suggests that the antibodies may cross-react with identical epitope sequences. 
      HCMV is not related with PCMV, and no correlation between antibody reactivity 
      against PCMV and HCMV was detected. These data indicate that antibodies against 
      PCMV found in humans are cross-reactive antibodies against HHV-6.
FAU - Fiebig, Uwe
AU  - Fiebig U
AD  - Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. FiebigU@rki.de.
FAU - Holzer, Angela
AU  - Holzer A
AD  - Institute of Virology, University Erlangen, Schlossgarten 4, 91054 Erlangen, 
      Germany. Angela.Holzer@viro.med.uni-erlangen.de.
FAU - Ivanusic, Daniel
AU  - Ivanusic D
AD  - Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. IvanusicD@rki.de.
FAU - Plotzki, Elena
AU  - Plotzki E
AD  - Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. elena.plotzki@gmx.de.
FAU - Hengel, Hartmut
AU  - Hengel H
AUID- ORCID: 0000-0002-3482-816X
AD  - Institute of Virology, Medical Center, Faculty of Medicine, University of 
      Freiburg, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany. 
      Hartmut.Hengel@uniklinik-freiburg.de.
FAU - Neipel, Frank
AU  - Neipel F
AD  - Institute of Virology, University Erlangen, Schlossgarten 4, 91054 Erlangen, 
      Germany. Frank.Neipel@viro.med.uni-erlangen.de.
FAU - Denner, Joachim
AU  - Denner J
AUID- ORCID: 0000-0003-3244-6085
AD  - Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany. DennerJ@rki.de.
LA  - eng
PT  - Journal Article
DEP - 20171028
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Blotting, Western
MH  - Cross Reactions
MH  - Cytomegalovirus/*immunology
MH  - Cytomegalovirus Infections/*immunology/virology
MH  - DNA, Viral/analysis
MH  - Herpesvirus 6, Human/*immunology
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Recombinant Proteins/immunology
MH  - Sequence Alignment
MH  - Swine
MH  - Viral Envelope Proteins/*immunology
PMC - PMC5707524
OTO - NOTNLM
OT  - human cytomegalovirus
OT  - human herpesvirus-6
OT  - porcine cytomegalovirus
OT  - virus transmission
OT  - xenotransplantation
COIS- The authors declare no conflict of interest.
EDAT- 2017/11/17 06:00
MHDA- 2018/11/24 06:00
PMCR- 2017/11/01
CRDT- 2017/11/17 06:00
PHST- 2017/07/25 00:00 [received]
PHST- 2017/10/10 00:00 [revised]
PHST- 2017/10/19 00:00 [accepted]
PHST- 2017/11/17 06:00 [entrez]
PHST- 2017/11/17 06:00 [pubmed]
PHST- 2018/11/24 06:00 [medline]
PHST- 2017/11/01 00:00 [pmc-release]
AID - v9110317 [pii]
AID - viruses-09-00317 [pii]
AID - 10.3390/v9110317 [doi]
PST - epublish
SO  - Viruses. 2017 Oct 28;9(11):317. doi: 10.3390/v9110317.