PMID- 29147397
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220310
IS  - 1920-454X (Electronic)
IS  - 1920-4531 (Print)
IS  - 1920-4531 (Linking)
VI  - 5
IP  - 4
DP  - 2014 Aug
TI  - Liquid-Based Medium Used to Prepare Cytological Breast Nipple Fluid Improves the 
      Quality of Cellular Samples Automatic Collection.
PG  - 166-174
LID - 10.14740/wjon844e [doi]
AB  - BACKGROUND: Breast cancer is the second cause of death in women worldwide. The 
      spontaneous breast nipple discharge may contain cells that can be analyzed for 
      malignancy. Halo((R)) Mamo Cyto Test (HMCT) was recently developed as an automated 
      system indicated to aspirate cells from the breast ducts. The objective of this 
      study was to standardize the methodology of sampling and sample preparation of 
      nipple discharge obtained by the automated method Halo breast test and perform 
      cytological evaluation in samples preserved in liquid medium (SurePath). 
      METHODS: We analyzed 564 nipple fluid samples, from women between 20 and 85 years 
      old, without history of breast disease and neoplasia, no pregnancy, and without 
      gynecologic medical history, collected by HMCT method and preserved in two 
      different vials with solutions for transport. RESULTS: From 306 nipple fluid 
      samples from method 1, 199 (65%) were classified as unsatisfactory (class 0), 104 
      (34%) samples were classified as benign findings (class II), and three (1%) were 
      classified as undetermined to neoplastic cells (class III). From 258 samples 
      analyzed in method 2, 127 (49%) were classified as class 0, 124 (48%) were 
      classified as class II, and seven (2%) were classified as class III. CONCLUSION: 
      Our study suggests an improvement in the quality and quantity of cellular samples 
      when the association of the two methodologies is performed, Halo breast test and 
      the method in liquid medium.
FAU - Zonta, Marco Antonio
AU  - Zonta MA
AD  - Infectology Department, Faculty of Medicine, Federal University of Sao Paulo; 
      Santo Amaro University, Brazil.
AD  - IN CITO - Citologia Diagnostica Lab, Sao Paulo, Brazil.
FAU - Velame, Fernanda
AU  - Velame F
AD  - IN CITO - Citologia Diagnostica Lab, Sao Paulo, Brazil.
FAU - Gema, Samara
AU  - Gema S
AD  - IN CITO - Citologia Diagnostica Lab, Sao Paulo, Brazil.
FAU - Filassi, Jose Roberto
AU  - Filassi JR
AD  - Mastology Section, Department of Gynecology, Faculty of Medicine, Sao Paulo 
      University, Brazil.
FAU - Longatto-Filho, Adhemar
AU  - Longatto-Filho A
AD  - Laboratory of Medical Investigation (LIM) 14, Department of Pathology, Faculty of 
      Medicine, Sao Paulo University, Brazil.
AD  - Life and Health Sciences Research Institute (ICVS), School of Health Sciences, 
      University of Minho, Braga, Portugal; ICVS/3B's - PT Government Associate 
      Laboratory, Braga/Guimaraes, Portugal; Molecular Oncology Research Center, 
      Barretos Cancer Hospital, Barretos, Sao Paulo, Brazil.
AD  - Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, 
      Barretos, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20140825
PL  - Canada
TA  - World J Oncol
JT  - World journal of oncology
JID - 101564097
PMC - PMC5649742
OTO - NOTNLM
OT  - Atypical ductal cells
OT  - Breast cytology
OT  - Ductal carcinoma
OT  - Liquid based cytology
EDAT- 2014/08/01 00:00
MHDA- 2014/08/01 00:01
PMCR- 2014/08/01
CRDT- 2017/11/18 06:00
PHST- 2014/07/04 00:00 [accepted]
PHST- 2017/11/18 06:00 [entrez]
PHST- 2014/08/01 00:00 [pubmed]
PHST- 2014/08/01 00:01 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.14740/wjon844e [doi]
PST - ppublish
SO  - World J Oncol. 2014 Aug;5(4):166-174. doi: 10.14740/wjon844e. Epub 2014 Aug 25.