PMID- 29149047
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2073-4425 (Print)
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 8
IP  - 11
DP  - 2017 Nov 17
TI  - New Insights into Phasmatodea Chromosomes.
LID - 10.3390/genes8110327 [doi]
LID - 327
AB  - Currently, approximately 3000 species of stick insects are known; however, 
      chromosome numbers, which range between 21 and 88, are known for only a few of 
      these insects. Also, centromere banding staining (C-banding) patterns were 
      described for fewer than 10 species, and fluorescence in situ hybridization 
      (FISH) was applied exclusively in two Leptynia species. Interestingly, 10-25% of 
      stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As 
      clonal and/or bisexual reproduction can affect chromosomal evolution, stick 
      insect karyotypes need to be studied more intensely. Chromosome preparation from 
      embryos of five Phasmatodea species (Medauroidea extradentata, Sungaya 
      inexpectata, Sipyloidea sipylus, Phaenopharos khaoyaiensis, and Peruphasma 
      schultei) from four families were studied here by C-banding and FISH applying 
      ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three 
      species, data on chromosome numbers and structure were obtained here for the 
      first time, i.e., S. inexpectata, P. khaoyaiensis, and P. schultei. Large 
      C-positive regions enriched with rDNA were identified in all five studied, 
      distantly related species. Some of these C-positive blocks were enriched for 
      telomeric repeats, as well. Chromosomal evolution of stick insects is 
      characterized by variations in chromosome numbers as well as transposition and 
      amplification of repetitive DNA sequences. Here, the first steps were made 
      towards identification of individual chromosomes in Phasmatodea.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Institute of Human Genetics, Jena University Hospital, Am Klinikum 1, D-07747 
      Jena, Germany. Thomas.Liehr@med.uni-jena.de.
FAU - Buleu, Olesya
AU  - Buleu O
AD  - Novosibirsk State University, 630090 Novosibirsk, Russia. buleu.olesya@mail.ru.
FAU - Karamysheva, Tatyana
AU  - Karamysheva T
AD  - Institute of Cytology and Genetics, Siberian Branch of Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia. kary@bionet.nsc.ru.
FAU - Bugrov, Alexander
AU  - Bugrov A
AD  - Novosibirsk State University, 630090 Novosibirsk, Russia. bugrov04@yahoo.co.uk.
AD  - Institute of Systematics and Ecology of Animals, Siberian Branch of Russian 
      Academy of Sciences, 630090 Novosibirsk, Russia. bugrov04@yahoo.co.uk.
FAU - Rubtsov, Nikolai
AU  - Rubtsov N
AD  - Novosibirsk State University, 630090 Novosibirsk, Russia. rubt@bionet.nsc.ru.
AD  - Institute of Cytology and Genetics, Siberian Branch of Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia. rubt@bionet.nsc.ru.
LA  - eng
PT  - Journal Article
DEP - 20171117
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
PMC - PMC5704240
OTO - NOTNLM
OT  - C-banding
OT  - Phasmatodea
OT  - fluorescence in situ hybridization
OT  - interstitial telomeric sequences
OT  - ribosomal deoxyribonucleic acid
OT  - stick insects
OT  - telomeric repeats
COIS- The authors declare no conflict of interest. The founding sponsors had no role in 
      the design of the study; in the collection, analyses, or interpretation of data; 
      in the writing of the manuscript, and in the decision to publish the results.
EDAT- 2017/11/18 06:00
MHDA- 2017/11/18 06:01
PMCR- 2017/11/01
CRDT- 2017/11/18 06:00
PHST- 2017/09/19 00:00 [received]
PHST- 2017/11/13 00:00 [revised]
PHST- 2017/11/13 00:00 [accepted]
PHST- 2017/11/18 06:00 [entrez]
PHST- 2017/11/18 06:00 [pubmed]
PHST- 2017/11/18 06:01 [medline]
PHST- 2017/11/01 00:00 [pmc-release]
AID - genes8110327 [pii]
AID - genes-08-00327 [pii]
AID - 10.3390/genes8110327 [doi]
PST - epublish
SO  - Genes (Basel). 2017 Nov 17;8(11):327. doi: 10.3390/genes8110327.