PMID- 29149643
OWN - NLM
STAT- MEDLINE
DCOM- 20180126
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 194
DP  - 2018 Jan
TI  - The role of the p38-activated protein kinase signaling pathway-mediated autophagy 
      in cadmium-exposed monogonont rotifer Brachious koreanus.
PG  - 46-56
LID - S0166-445X(17)30318-1 [pii]
LID - 10.1016/j.aquatox.2017.11.002 [doi]
AB  - Autophagy is a 'self-eating' system that regulates the degradation of cellular 
      components and is involved in various biological processes including survival and 
      development. However, despite its crucial role in organisms, the regulatory 
      mechanism of autophagy remains largely unclear, particularly in invertebrates. In 
      this study, conserved autophagy in the rotifer Brachionus koreanus in response to 
      cadmium (Cd) exposure was verified by measuring acidic vesicle organelles using 
      acridine orange (AO) and neutral red (NR) staining, and by detecting LC3 I/II on 
      Western blot and immunofluorescence. We also demonstrated activation of p38 
      mitogen-activated protein kinase (MAPK) in response to Cd-induced oxidative 
      stress, leading to the induction of autophagy in B. koreanus. This was further 
      verified by analysis of MAPK protein levels and immunofluorescence of LC3 I/II 
      after treatment with reactive oxygen species (ROS) scavengers and inhibitors 
      specific to MAPKs. We propose a p38 MAPK-mediated regulatory mechanism of 
      autophagy in B. koreanus in response to Cd-induced oxidative stress. This study 
      will contribute to a better understanding of autophagic processes in 
      invertebrates and its modulation by environmental stressors.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Kang, Hye-Min
AU  - Kang HM
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea.
FAU - Jeong, Chang-Bum
AU  - Jeong CB
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea.
FAU - Kim, Min-Sub
AU  - Kim MS
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea.
FAU - Lee, Jin-Sol
AU  - Lee JS
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea; Department of Chemistry, College of Science, Duksung 
      Woman's University, Seoul 01369, South Korea.
FAU - Zhou, Jiaying
AU  - Zhou J
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea.
FAU - Lee, Young Hwan
AU  - Lee YH
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea.
FAU - Kim, Duck-Hyun
AU  - Kim DH
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea.
FAU - Moon, Eunyoung
AU  - Moon E
AD  - Electron Microscopy Research Center, Korea Basic Science Institute, Daejeon 
      34133, South Korea.
FAU - Kweon, Hee-Seok
AU  - Kweon HS
AD  - Electron Microscopy Research Center, Korea Basic Science Institute, Daejeon 
      34133, South Korea.
FAU - Lee, Su-Jae
AU  - Lee SJ
AD  - Department of Life Science, College of Natural Sciences, Hanyang University, 
      Seoul 04763, South Korea.
FAU - Lee, Jae-Seong
AU  - Lee JS
AD  - Department of Biological Science, College of Science, Sungkyunkwan University, 
      Suwon 16419, South Korea. Electronic address: jslee2@skku.edu.
LA  - eng
PT  - Journal Article
DEP - 20171110
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Helminth Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Water Pollutants, Chemical)
RN  - 00BH33GNGH (Cadmium)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autophagy/*drug effects
MH  - Cadmium/metabolism/*toxicity
MH  - Helminth Proteins/metabolism
MH  - Humans
MH  - MAP Kinase Signaling System/*drug effects
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Microtubule-Associated Proteins/chemistry/metabolism
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Oxidative Stress/drug effects
MH  - Phosphorylation/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Rotifera/drug effects/growth & development/*metabolism
MH  - Sequence Alignment
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Autophagy
OT  - LC3 I/II
OT  - Mitogen-activated protein kinases
OT  - Rotifer
OT  - p38 MAPK
EDAT- 2017/11/18 06:00
MHDA- 2018/01/27 06:00
CRDT- 2017/11/18 06:00
PHST- 2017/10/16 00:00 [received]
PHST- 2017/11/06 00:00 [revised]
PHST- 2017/11/08 00:00 [accepted]
PHST- 2017/11/18 06:00 [pubmed]
PHST- 2018/01/27 06:00 [medline]
PHST- 2017/11/18 06:00 [entrez]
AID - S0166-445X(17)30318-1 [pii]
AID - 10.1016/j.aquatox.2017.11.002 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2018 Jan;194:46-56. doi: 10.1016/j.aquatox.2017.11.002. Epub 2017 
      Nov 10.