PMID- 29153954
OWN - NLM
STAT- MEDLINE
DCOM- 20180727
LR  - 20180727
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 54
DP  - 2018 Jan
TI  - MiR-150-5p regulates EGR2 to promote the development of chronic rhinosinusitis 
      via the DC-Th axis.
PG  - 188-197
LID - S1567-5769(17)30431-9 [pii]
LID - 10.1016/j.intimp.2017.11.011 [doi]
AB  - BACKGROUND AND AIMS: Accumulating studies indicate that miR-150-5p might play a 
      significant role in dendritic cells (DCs) of peripheral blood in chronic 
      rhinosinusitis (CRS) patients. We sought to investigate the effects and mechanism 
      of miR-150-5p, which regulates early growth response 2 (EGR2) to promote the 
      development of CRS via the DC-Th axis. METHODS: The upregulated expression of 
      miR-150-5p in DCs of CRS was assayed by real-time quantitative polymerase chain 
      reaction (qRT-PCR), and IL-17 cytokines in the supernatants of DC-naive T cells 
      co-cultures were analysed by enzyme-linked immunosorbent assay (ELISA). Flow 
      cytometry was used to evaluate T cell proliferations. EGR2 was also identified as 
      a direct target of miR-150-5p by establishing a miRNA-mRNA network, and this 
      target was validated with a Dual-Luciferase(R) Reporter Assay System and Western 
      blot. RESULTS: MiR-150-5p was up-regulated in DCs in peripheral blood from CRS 
      patients, and this expression was down-regulated by EGR2 expression via the DC-Th 
      axis. Up-regulated miR-150-5p Regulates DCs, and DCs Promote Naive T Cells 
      Proliferation. MiR-150-5p Further Regulates EGR2 and Inhibits DCs, Which Makes 
      the DC-Th Axis Abnormal in the Peripheral Blood of Patients with CRS. CONCLUSION: 
      MiR-150-5p and its identified target, EGR2, are involved in the development of 
      CRS. DCs can promote T cell proliferations of peripheral blood in CRS.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Ma, Zuxia
AU  - Ma Z
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China; Department of Otorhinolaryngology, Zunyi 
      First People's Hospital, Zunyi, China.
FAU - Shen, Yang
AU  - Shen Y
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China.
FAU - Zeng, Quan
AU  - Zeng Q
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China.
FAU - Liu, Jie
AU  - Liu J
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China.
FAU - Yang, Li
AU  - Yang L
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China.
FAU - Fu, Ran
AU  - Fu R
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China.
FAU - Hu, Guohua
AU  - Hu G
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing 
      Medical University,Chongqing, China. Electronic address: hghcq@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20171116
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (MIRN150 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chronic Disease
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Disease Progression
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Rhinitis/*genetics
MH  - Sinusitis/*genetics
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Chronic rhinosinusitis
OT  - Dendritic cells
OT  - EGR2
OT  - Naive T cells
OT  - miR-150-5p
EDAT- 2017/11/21 06:00
MHDA- 2018/07/28 06:00
CRDT- 2017/11/21 06:00
PHST- 2017/05/04 00:00 [received]
PHST- 2017/10/28 00:00 [revised]
PHST- 2017/11/08 00:00 [accepted]
PHST- 2017/11/21 06:00 [pubmed]
PHST- 2018/07/28 06:00 [medline]
PHST- 2017/11/21 06:00 [entrez]
AID - S1567-5769(17)30431-9 [pii]
AID - 10.1016/j.intimp.2017.11.011 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2018 Jan;54:188-197. doi: 10.1016/j.intimp.2017.11.011. Epub 
      2017 Nov 16.