PMID- 29154391
OWN - NLM
STAT- MEDLINE
DCOM- 20180108
LR  - 20180108
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 126
IP  - 1
DP  - 2018 Jan
TI  - The value of microscopic-observation drug susceptibility assay in the diagnosis 
      of tuberculosis and detection of multidrug resistance.
PG  - 38-44
LID - 10.1111/apm.12783 [doi]
AB  - Inexpensive, rapid, and reliable tests for detecting the presence and drug 
      susceptibility of Mycobacterium tuberculosis complex (MTBC) are urgently needed 
      to control the transmission of tuberculosis. In this study, we aimed to assess 
      the accuracy and speed of the microscopic-observation drug susceptibility (MODS) 
      assay in the identification of MTBC and detection of multidrug resistance. Sputum 
      samples from patients suspected to have tuberculosis were simultaneously tested 
      with MODS and conventional culture [Lowenstein-Jensen (LJ) culture, BACTEC MGIT 
      960 (MGIT) system], and drug susceptibility testing (MGIT system) methods. A 
      total of 331 sputum samples were analyzed. Sensitivity and specificity of MODS 
      assay for detection of MTBC strains were 96% and 98.8%, respectively. MODS assay 
      detected multidrug resistant MTBC isolates with 92.3% sensitivity and 96.6% 
      specificity. Median time to culture positivity was similar for MGIT (8 days) and 
      MODS culture (8 days), but was significantly longer with LJ culture (20 days) 
      (p < 0.0001 for both comparisons). Median time to availability of the 
      susceptibility results was significantly (p < 0.0001) shorter with MODS assay 
      (8 days) than MGIT system (20 days). In conclusion, MODS is an inexpensive and 
      rapid test with good performance characteristics for direct diagnosis of 
      tuberculosis and detection of multidrug resistance.
CI  - (c) 2017 APMIS. Published by John Wiley & Sons Ltd.
FAU - Sertel Selale, DenIz
AU  - Sertel Selale D
AD  - Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
AD  - Department of Medical Microbiology, International School of Medicine, Istanbul 
      Medipol University, Istanbul, Turkey.
FAU - Uzun, Meltem
AU  - Uzun M
AD  - Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20171120
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
SB  - IM
MH  - Drug Resistance, Multiple, Bacterial
MH  - Humans
MH  - *Microbial Sensitivity Tests
MH  - *Microscopy
MH  - Sputum/microbiology
MH  - Tuberculosis/*diagnosis/drug therapy
OTO - NOTNLM
OT  - Mycobacterium tuberculosis
OT  - isoniazid
OT  - multidrug resistant tuberculosis
OT  - rifampicin
EDAT- 2017/11/21 06:00
MHDA- 2018/01/09 06:00
CRDT- 2017/11/21 06:00
PHST- 2017/02/13 00:00 [received]
PHST- 2017/09/18 00:00 [accepted]
PHST- 2017/11/21 06:00 [pubmed]
PHST- 2018/01/09 06:00 [medline]
PHST- 2017/11/21 06:00 [entrez]
AID - 10.1111/apm.12783 [doi]
PST - ppublish
SO  - APMIS. 2018 Jan;126(1):38-44. doi: 10.1111/apm.12783. Epub 2017 Nov 20.