PMID- 29155090
OWN - NLM
STAT- MEDLINE
DCOM- 20190807
LR  - 20190807
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Linking)
VI  - 180
DP  - 2018 May 30
TI  - Proteome and phosphoproteome analysis of commensally induced dendritic cell 
      maturation states.
PG  - 11-24
LID - S1874-3919(17)30380-9 [pii]
LID - 10.1016/j.jprot.2017.11.008 [doi]
AB  - Dendritic cells (DCs) can shape the immune system towards an inflammatory or 
      tolerant state depending on the bacterial antigens and the environment they 
      encounter. In this study we provide a proteomic catalogue of differentially 
      expressed proteins between distinct DC maturation states, brought about by 
      bacteria that differ in their endotoxicity. To achieve this, we have performed 
      proteomics and phosphoproteomics on murine DC cultures. Symbiont and pathobiont 
      bacteria were used to direct dendritic cells into a semi-mature and fully-mature 
      state, respectively. The comparison of semi-mature and fully-mature DCs revealed 
      differential expression in 103 proteins and differential phosphorylation in 118 
      phosphosites, including major regulatory factors of central immune processes. Our 
      analyses predict that these differences are mediated by upstream elements such as 
      SOCS1, IRF3, ABCA1, TLR4, and PTGER4. Our analyses indicate that the symbiont 
      bacterial strain affects DC proteome in a distinct way, by downregulating 
      inflammatory proteins and activating anti-inflammatory upstream regulators. 
      Biological significance In this study we have investigated the responses of 
      immune cells to distinct bacterial stimuli. We have used the symbiont bacterial 
      strain B. vulgatus and the pathobiont E. coli strain to stimulate cultured 
      primary dendritic cells and performed a shotgun proteome analysis to investigate 
      the protein expression and phosphorylation level differences on a genome level. 
      We have observed expression and phosphorylation level differences in key immune 
      regulators, transcription factors and signal transducers. Moreover, our 
      subsequent bioinformatics analysis indicated regulation at several signaling 
      pathways such as PPAR signaling, LXR/RXR activation and glucocorticoid signaling 
      pathways, which are not studied in detail in an inflammation and DC maturation 
      context. Our phosphoproteome analysis showed differential phosphorylation in 118 
      phosphosites including those belonging to epigenetic regulators, transcription 
      factors and major cell cycle regulators. We anticipate that our study will 
      facilitate further investigation of immune cell proteomes under different 
      inflammatory and non-inflammatory conditions.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Korkmaz, Ali Giray
AU  - Korkmaz AG
AD  - Institute of Medical Microbiology and Hygiene, University of Tubingen, Germany. 
      Electronic address: giray.korkmaz@med.uni-tuebingen.de.
FAU - Popov, Todor
AU  - Popov T
AD  - Institute of Medical Microbiology and Hygiene, University of Tubingen, Germany.
FAU - Peisl, Loulou
AU  - Peisl L
AD  - Institute of Medical Microbiology and Hygiene, University of Tubingen, Germany.
FAU - Codrea, Marius Cosmin
AU  - Codrea MC
AD  - Quantitative Biology Center, University of Tubingen, Germany.
FAU - Nahnsen, Sven
AU  - Nahnsen S
AD  - Quantitative Biology Center, University of Tubingen, Germany.
FAU - Steimle, Alexander
AU  - Steimle A
AD  - Institute of Medical Microbiology and Hygiene, University of Tubingen, Germany.
FAU - Velic, Ana
AU  - Velic A
AD  - Proteome Center, University of Tubingen, Germany.
FAU - Macek, Boris
AU  - Macek B
AD  - Proteome Center, University of Tubingen, Germany.
FAU - von Bergen, Martin
AU  - von Bergen M
AD  - Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.
FAU - Bernhardt, Joerg
AU  - Bernhardt J
AD  - Ernst-Moritz-Arndt Universitat Greifswald, Institute for Microbiology, Germany.
FAU - Frick, Julia-Stefanie
AU  - Frick JS
AD  - Institute of Medical Microbiology and Hygiene, University of Tubingen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20171115
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteome)
MH  - Animals
MH  - *Bacteroides
MH  - Bacteroides Infections/*metabolism
MH  - Dendritic Cells/*metabolism/pathology
MH  - *Escherichia coli
MH  - Escherichia coli Infections/*metabolism
MH  - Female
MH  - Mice
MH  - Phosphoproteins/*biosynthesis
MH  - Proteome/*biosynthesis
MH  - Proteomics
OTO - NOTNLM
OT  - B. vulgatus
OT  - Commensals
OT  - Dendritic cells
OT  - Expression analysis
OT  - Inflammation
OT  - Shotgun proteomics
EDAT- 2017/11/21 06:00
MHDA- 2019/08/08 06:00
CRDT- 2017/11/21 06:00
PHST- 2017/02/17 00:00 [received]
PHST- 2017/09/18 00:00 [revised]
PHST- 2017/11/14 00:00 [accepted]
PHST- 2017/11/21 06:00 [pubmed]
PHST- 2019/08/08 06:00 [medline]
PHST- 2017/11/21 06:00 [entrez]
AID - S1874-3919(17)30380-9 [pii]
AID - 10.1016/j.jprot.2017.11.008 [doi]
PST - ppublish
SO  - J Proteomics. 2018 May 30;180:11-24. doi: 10.1016/j.jprot.2017.11.008. Epub 2017 
      Nov 15.