PMID- 29155093 OWN - NLM STAT- MEDLINE DCOM- 20190401 LR - 20200930 IS - 1872-6240 (Electronic) IS - 0006-8993 (Linking) VI - 1688 DP - 2018 Jun 1 TI - Histone acetylation of oligodendrocytes protects against white matter injury induced by inflammation and hypoxia-ischemia through activation of BDNF-TrkB signaling pathway in neonatal rats. PG - 33-46 LID - S0006-8993(17)30500-0 [pii] LID - 10.1016/j.brainres.2017.11.005 [doi] AB - The major pathological damage in encephalopathy of prematurity is white matter injury (WMI). Perinatal hypoxic-ischemia (HI) and inflammation are two major risk factors in the development of WMI. To study the cellular and molecular mechanisms of WMI, we set up a WMI model using lipopolysaccharide-sensitized HI injury in 2-day postnatal rats. Immunofluorescence staining was used to measure the expression of acetylated histone H3 (AH3) in oligodendrocytes, the target cells of WMI; the oligodendrocyte protein markers, NG2, O4, MBP, PLP, and MAG, were detected at different developmental stages. 5-bromo-2'-deoxyuridine (BrdU) was used to detect the proliferation of oligodendrocytes. We found that the expression of AH3 was markedly decreased in oligodendrocytes at 7 days after WMI. The differentiation and maturation of oligodendrocytes were inhibited in the WMI group. After inducing histone acetylation in oligodendrocytes by treatment with sodium butyrate, the inhibition of differentiation and maturation of oligodendrocytes was reversed. Furthermore, we found that these protective effects of histone acetylation were associated with the upregulation of brain-derived neurotrophic factor (BDNF) and its receptor, tyrosine kinase B (TrkB). In conclusion, histone acetylation protects oligodendrocytes from WMI through activation of the BDNF-TrkB signaling pathway in immature brains. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Huang, Jichong AU - Huang J AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Zhang, Li AU - Zhang L AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Qu, Yi AU - Qu Y AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Zhou, Yan AU - Zhou Y AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Zhu, Jianghu AU - Zhu J AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Li, Yafei AU - Li Y AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Zhu, Tingting AU - Zhu T AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Zhao, Fengyan AU - Zhao F AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Tang, Jun AU - Tang J AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. Electronic address: tj1234753@sina.com. FAU - Mu, Dezhi AU - Mu D AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. Electronic address: mudz@scu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171116 PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (Bdnf protein, rat) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Histones) RN - EC 2.7.10.1 (Ntrk2 protein, rat) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Acetylation MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cell Differentiation MH - Cell Proliferation MH - Encephalitis/complications/*metabolism/pathology MH - Female MH - Histones/*metabolism MH - Hypoxia-Ischemia, Brain/complications/*metabolism/pathology MH - Male MH - Myelin Sheath/metabolism MH - Oligodendroglia/*metabolism MH - Rats, Sprague-Dawley MH - Receptor, trkB/*metabolism MH - Signal Transduction MH - White Matter/*pathology OTO - NOTNLM OT - Brain-derived neurotrophic factor OT - Histone acetylation OT - Oligodendrocytes OT - Sodium butyrate OT - White matter injury EDAT- 2017/11/21 06:00 MHDA- 2019/04/02 06:00 CRDT- 2017/11/21 06:00 PHST- 2017/06/07 00:00 [received] PHST- 2017/09/08 00:00 [revised] PHST- 2017/11/06 00:00 [accepted] PHST- 2017/11/21 06:00 [pubmed] PHST- 2019/04/02 06:00 [medline] PHST- 2017/11/21 06:00 [entrez] AID - S0006-8993(17)30500-0 [pii] AID - 10.1016/j.brainres.2017.11.005 [doi] PST - ppublish SO - Brain Res. 2018 Jun 1;1688:33-46. doi: 10.1016/j.brainres.2017.11.005. Epub 2017 Nov 16.