PMID- 29155303
OWN - NLM
STAT- MEDLINE
DCOM- 20180108
LR  - 20180108
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 192
DP  - 2018 Jan 1
TI  - Therapeutic vaccine against IL-1beta improved glucose control in a mouse model of 
      type 2 diabetes.
PG  - 68-74
LID - S0024-3205(17)30599-4 [pii]
LID - 10.1016/j.lfs.2017.11.021 [doi]
AB  - AIMS: Inflammation is strongly associated with the mechanism of beta-cell failure in 
      type 2 diabetes mellitus (T2DM). Blockade of the key proinflammatory cytokine 
      IL-1beta has been implicated as a promising therapeutic strategy for the prevention 
      and treatment of type 2 diabetes. In this study, we developed an IL-1beta-targeted 
      therapeutic vaccine consisting of an IL-1beta epitope peptide (A1beta) and assessed its 
      efficacy on a diabetic KK-A(y) mouse model. MAIN METHODS: KK-A(y) mice were 
      immunized with A1beta for three injections at a 2-week interval. The induced 
      antibody titers, body weights and blood glucose levels were monitored every two 
      weeks. Then the intraperitoneal glucose tolerance test and insulin tolerance test 
      were performed. The beta-cell mass, beta-cell apoptosis and proliferation were 
      evaluated by immunofluorescence. IL-1beta gene expression in islets was also 
      measured by quantitative RT-PCR. KEY FINDINGS: A1beta immunization induced robust 
      antibody responses, reduced body weight gain, improved glucose tolerance and 
      insulin sensitivity in KK-A(y) mice. Moreover, A1beta restored beta-cell mass, 
      inhibited beta-cell apoptosis, enhanced beta-cell proliferation and downregulated IL-1beta 
      expression. SIGNIFICANCE: The novel IL-1beta-targeted epitope vaccine has the 
      therapeutic potential for T2DM.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, Taian, Shandong, China; State 
      Key Laboratory of Biochemical Engineering, Institute of Process Engineering, 
      Chinese Academy of Sciences, Beijing, China.
FAU - Yu, Xiao-Lin
AU  - Yu XL
AD  - State Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China.
FAU - Zha, Jun
AU  - Zha J
AD  - State Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China.
FAU - Mao, Li-Zhen
AU  - Mao LZ
AD  - Asialand Biomed Technology Co., Ltd., Changzhou, China.
FAU - Chai, Jia-Qian
AU  - Chai JQ
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, Taian, Shandong, China. 
      Electronic address: jqchai@sdau.edu.cn.
FAU - Liu, Rui-Tian
AU  - Liu RT
AD  - State Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, China. Electronic address: 
      rtliu@ipe.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20171116
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Antibodies)
RN  - 0 (Blood Glucose)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies/analysis
MH  - Apoptosis
MH  - Blood Glucose/*metabolism
MH  - Body Weight
MH  - Cell Proliferation
MH  - Diabetes Mellitus, Type 2/genetics/*metabolism/*therapy
MH  - Disease Models, Animal
MH  - Down-Regulation/genetics/immunology
MH  - Epitopes/immunology
MH  - Glucose Tolerance Test
MH  - Immunoglobulin G/analysis/biosynthesis
MH  - Insulin Resistance
MH  - Insulin-Secreting Cells/metabolism
MH  - Interleukin-1beta/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Vaccines/immunology/*therapeutic use
OTO - NOTNLM
OT  - IL-1beta
OT  - Islet inflammation
OT  - Therapeutic vaccine
OT  - Type 2 diabetes
EDAT- 2017/11/21 06:00
MHDA- 2018/01/09 06:00
CRDT- 2017/11/21 06:00
PHST- 2017/09/05 00:00 [received]
PHST- 2017/11/03 00:00 [revised]
PHST- 2017/11/15 00:00 [accepted]
PHST- 2017/11/21 06:00 [pubmed]
PHST- 2018/01/09 06:00 [medline]
PHST- 2017/11/21 06:00 [entrez]
AID - S0024-3205(17)30599-4 [pii]
AID - 10.1016/j.lfs.2017.11.021 [doi]
PST - ppublish
SO  - Life Sci. 2018 Jan 1;192:68-74. doi: 10.1016/j.lfs.2017.11.021. Epub 2017 Nov 16.