PMID- 29155323
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1090-2139 (Electronic)
IS  - 0889-1591 (Linking)
VI  - 69
DP  - 2018 Mar
TI  - The role of P2X7R/ERK signaling in dorsal root ganglia satellite glial cells in 
      the development of chronic postsurgical pain induced by skin/muscle incision and 
      retraction (SMIR).
PG  - 180-189
LID - S0889-1591(17)30512-3 [pii]
LID - 10.1016/j.bbi.2017.11.011 [doi]
AB  - The mechanisms of chronic postsurgical pain remain to be elucidated. We reported 
      here that skin/muscle incision and retraction (SMIR), a rat model of postsurgical 
      pain, phosphorylated the extracellular regulated protein kinases (ERK) signaling 
      components c-Raf, MEK (ERK kinase) and ERK1/2 in lumbar 3 dorsal root ganglion 
      (L3 DRG) in rats. Intrathecal injection of ERK specific inhibitor SCH772984 
      suppressed the mechanical allodynia induced by SMIR. Furthermore, SMIR 
      upregulated tumor necrosis factor alpha (TNFalpha) in L3 DRG, which could be 
      inhibited by SCH772984. Intrathecal injection of TNF antagonist Etanercept could 
      also inhibit the mechanical allodynia and the increased ERK phosphorylation in L3 
      DRG induced by SMIR. In addition, immunofluorescent data showed that P2X7R was 
      located exclusively in GFAP labeled satellite glial cells and was highly 
      colocalized with p-ERK1/2 following SMIR. Pretreatment with P2X7R antagonist 
      Brilliant Blue G (BBG) could also block the mechanical allodynia, inhibited the 
      phosphorylation of c-Raf, MEK, ERK1/2, and decrease the expression of TNF-alpha. 
      Finally, intrathecal injection of BzATP produced mechanical allodynia and induced 
      ERK phosphorylation in satellite glial cells in L3 DRG. Thus, P2X7R activation in 
      satellite glial cells in L3 DRG, leading to a positive feedback between ERK 
      pathway activation and TNF-alpha production, is suggested to be involved in the 
      induction of chronic postsurgical pain following SMIR.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Song, Jingnian
AU  - Song J
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China; Department of 
      Anesthesiology, Hainan General Hospital, Xiuhua Rd., Haikou, Hainan 570311, 
      China.
FAU - Ying, Yanlu
AU  - Ying Y
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine 
      Sun Yat-sen University, Guangzhou 510080, China.
FAU - Liu, Xianguo
AU  - Liu X
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine 
      Sun Yat-sen University, Guangzhou 510080, China; Guangdong Provincial Key 
      Laboratory of Brain Function and Disease, Guangzhou, Guangdong, China.
FAU - Xu, Xuebing
AU  - Xu X
AD  - Department of Anesthesiology, The University of Hong Kong-Shenzhen Hospital, 
      Haiyuan 1st Road, Futian District, Shenzhen 518053, China. Electronic address: 
      xuxb@hku-szh.org.
FAU - Wei, Xuhong
AU  - Wei X
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine 
      Sun Yat-sen University, Guangzhou 510080, China; Guangdong Provincial Key 
      Laboratory of Brain Function and Disease, Guangzhou, Guangdong, China. Electronic 
      address: weixhong@mail.sysu.edu.cn.
FAU - Ruan, Xiangcai
AU  - Ruan X
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China. Electronic 
      address: xc_ruan@gzhmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171117
PL  - Netherlands
TA  - Brain Behav Immun
JT  - Brain, behavior, and immunity
JID - 8800478
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indazoles)
RN  - 0 (Piperazines)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 0 (Rosaniline Dyes)
RN  - 0 (SCH772984)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - M1ZRX790SI (coomassie Brilliant Blue)
SB  - IM
MH  - Animals
MH  - Enzyme Inhibitors/pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Ganglia, Spinal/drug effects/*metabolism
MH  - Hyperalgesia/*metabolism
MH  - Indazoles/pharmacology
MH  - Male
MH  - Models, Animal
MH  - Pain Measurement
MH  - Pain, Postoperative/etiology/*metabolism
MH  - Phosphorylation/drug effects
MH  - Piperazines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X7/*metabolism
MH  - Rosaniline Dyes/pharmacology
MH  - Satellite Cells, Perineuronal/drug effects/*metabolism
MH  - Signal Transduction/drug effects/*physiology
MH  - Surgical Wound/complications/metabolism
OTO - NOTNLM
OT  - Chronic postsurgical pain
OT  - Dorsal root ganglia
OT  - Extracellular signal-regulated kinase
OT  - P2X7 receptor
OT  - Tumor necrosis factor alpha
EDAT- 2017/11/21 06:00
MHDA- 2019/04/16 06:00
CRDT- 2017/11/21 06:00
PHST- 2017/07/19 00:00 [received]
PHST- 2017/11/16 00:00 [revised]
PHST- 2017/11/16 00:00 [accepted]
PHST- 2017/11/21 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2017/11/21 06:00 [entrez]
AID - S0889-1591(17)30512-3 [pii]
AID - 10.1016/j.bbi.2017.11.011 [doi]
PST - ppublish
SO  - Brain Behav Immun. 2018 Mar;69:180-189. doi: 10.1016/j.bbi.2017.11.011. Epub 2017 
      Nov 17.