PMID- 29156571
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20181207
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 22
IP  - 11
DP  - 2017 Nov 18
TI  - Studies for Improving a Rat Model of Alzheimer's Disease: Icv Administration of 
      Well-Characterized beta-Amyloid 1-42 Oligomers Induce Dysfunction in Spatial Memory.
LID - 10.3390/molecules22112007 [doi]
LID - 2007
AB  - During the past 15 years, several genetically altered mouse models of human 
      Alzheimer's disease (AD) have been developed. These costly models have greatly 
      facilitated the evaluation of novel therapeutic approaches. Injecting synthetic 
      beta-amyloid (Abeta) 1-42 species into different parts of the brain of non-transgenic 
      rodents frequently provided unreliable results, owing to a lack of a genuine 
      characterization of the administered Abeta aggregates. Previously, we have published 
      a new rat AD-model in which protofibrillar-fibrillar Abeta1-42 was administered into 
      rat entorhinal cortex (Sipos 2007). In order to develop a more reliable model, we 
      have injected well-characterized toxic soluble Abeta1-42 species (oligomers, 
      protofibrils and fibrils) intracerebroventricularly (icv) into rat brain. Studies 
      of the distribution of fluorescent-labeled Abeta1-42 in the brain showed that 
      soluble Abeta-species diffused into all parts of the rat brain. After seven days, 
      the Abeta-treated animals showed a significant decrease of spatial memory in Morris 
      water maze test and impairment of synaptic plasticity (LTP) measured in acute 
      hippocampal slices. The results of histological studies (decreased number of 
      viable neurons, increased tau levels and decreased number of dendritic spines) 
      also supported that icv administration of well-characterized toxic soluble Abeta 
      species into rat brain provides a reliable rat AD-model.
FAU - Kasza, Agnes
AU  - Kasza A
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. kaszagi@gmail.com.
FAU - Penke, Botond
AU  - Penke B
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. penke.botond@med.u-szeged.hu.
FAU - Frank, Zsuzsanna
AU  - Frank Z
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. frankzsu@gmail.com.
FAU - Bozso, Zsolt
AU  - Bozso Z
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. bozso.zsolt@med.u-szeged.hu.
FAU - Szegedi, Viktor
AU  - Szegedi V
AUID- ORCID: 0000-0003-4191-379X
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. szegv@yahoo.com.
FAU - Hunya, Akos
AU  - Hunya A
AD  - LipidArt Research and Development Ltd., Temesvari krt. 62, Szeged H-6726, 
      Hungary. akos.hunya@lipidart.com.
FAU - Nemeth, Klaudia
AU  - Nemeth K
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. klausz20@gmail.com.
FAU - Kozma, Gabor
AU  - Kozma G
AD  - Department of Applied and Environmental Chemistry, University of Szeged, Rerrich 
      Bela square 1, Szeged H-6720, Hungary. kozmag@chem.u-szeged.hu.
FAU - Fulop, Livia
AU  - Fulop L
AD  - Department of Medical Chemistry, University of Szeged, Dome square 8, Szeged 
      H-6720, Hungary. fulop.livia@med.u-szeged.hu.
LA  - eng
PT  - Journal Article
DEP - 20171118
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Amyloid beta-Peptides)
SB  - IM
MH  - Alzheimer Disease/*drug therapy/physiopathology
MH  - Amyloid beta-Peptides/administration & dosage/*therapeutic use
MH  - Animals
MH  - Disease Models, Animal
MH  - Male
MH  - Maze Learning/drug effects
MH  - Rats
MH  - Spatial Memory/*drug effects
PMC - PMC6150403
OTO - NOTNLM
OT  - AD rat model
OT  - Golgi staining
OT  - Morris water maze
OT  - amyloid beta
OT  - hippocampus
OT  - icv administration
OT  - long-term potentiation
OT  - spatial memory
COIS- The authors declare no conflict of interest.
EDAT- 2017/11/22 06:00
MHDA- 2018/07/03 06:00
PMCR- 2017/11/18
CRDT- 2017/11/22 06:00
PHST- 2017/10/09 00:00 [received]
PHST- 2017/11/07 00:00 [revised]
PHST- 2017/11/13 00:00 [accepted]
PHST- 2017/11/22 06:00 [entrez]
PHST- 2017/11/22 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
PHST- 2017/11/18 00:00 [pmc-release]
AID - molecules22112007 [pii]
AID - molecules-22-02007 [pii]
AID - 10.3390/molecules22112007 [doi]
PST - epublish
SO  - Molecules. 2017 Nov 18;22(11):2007. doi: 10.3390/molecules22112007.