PMID- 29158167
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20171226
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 642
DP  - 2018 Feb 5
TI  - ClC-3 chloride channel is involved in isoprenaline-induced cardiac hypertrophy.
PG  - 335-342
LID - S0378-1119(17)31011-9 [pii]
LID - 10.1016/j.gene.2017.11.045 [doi]
AB  - Isoprenaline, an activator of beta-adrenergic receptor, has been found to induce 
      cardiac hypertrophy in vivo and in vitro, but the exact mechanism is still 
      unclear. ClC-3 is a member of the chloride channel family and is highly expressed 
      in mammalian myocardium. In the present study, the role of ClC-3 in 
      isopronaline-induced cardiac hypertrophy was investigated. We found that ClC-3 
      expression was reduced in isoprenaline-induced hypertrophic H9c2 cells, primary 
      rat neonatal cardiomyocytes and myocardium of C57/BL/6 mice, and this reduction 
      was prevented by the pretreatment of propranolol. Adeno-associated virus 9 
      (AAV9)-mediated ClC-3 expression in myocardium decreased heart mass index, 
      thinned interventricular septum and left ventricular wall and lowered the mRNA 
      expression of natriuretic peptide type A (ANF) and beta-myosin heavy chain (beta-MHC). 
      Our results showed that ClC-3 played an important role in beta-adrenergic cardiac 
      hypertrophy which could be associated with ANF and beta-MHC, and all these findings 
      suggested that ClC-3 may be a novel therapeutic target for the prevention or 
      treatment of myocardiac hypertrophy.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Li, Chunmei
AU  - Li C
AD  - Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical 
      University, Guangzhou 510006, China; Guangdong Provincial Key Laboratory of 
      Pharmaceutical Bioactive Substances and School of Basic Medicine, Guangdong 
      Pharmaceutical University, Guangzhou 510006, China.
FAU - Huang, Dan
AU  - Huang D
AD  - Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances and 
      School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, 
      China; Hunan Traditional Chinese Medical College, Zhuzhou, 412012, China.
FAU - Tang, Jing
AU  - Tang J
AD  - Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances and 
      School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, 
      China.
FAU - Chen, Mengqing
AU  - Chen M
AD  - Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical 
      University, Guangzhou 510006, China.
FAU - Lu, Qun
AU  - Lu Q
AD  - Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical 
      University, Guangzhou 510006, China.
FAU - Li, He
AU  - Li H
AD  - Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical 
      University, Guangzhou 510006, China.
FAU - Zhang, Mengzhen
AU  - Zhang M
AD  - Guangdong General Hospital, Guangzhou 510000, China.
FAU - Xu, Bin
AU  - Xu B
AD  - Guangdong Province Key Laboratory for Biotechnology Drug Candidates and School of 
      Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 
      510006, China. Electronic address: xubin@gdpu.edu.cn.
FAU - Mao, Jianwen
AU  - Mao J
AD  - Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances and 
      School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, 
      China. Electronic address: shengwu@gdpu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20171120
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Chloride Channels)
RN  - 0 (ClC-3 channel)
RN  - 9Y8NXQ24VQ (Propranolol)
RN  - EC 3.6.1.- (Ventricular Myosins)
RN  - EC 4.6.1.2 (Receptors, Atrial Natriuretic Factor)
RN  - EC 4.6.1.2 (atrial natriuretic factor receptor A)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cardiomegaly/chemically induced/genetics/*metabolism/prevention & control
MH  - Cell Line
MH  - Chloride Channels/genetics/*metabolism
MH  - Dependovirus/genetics
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Isoproterenol/*adverse effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myocytes, Cardiac/cytology/drug effects/*metabolism
MH  - Propranolol/pharmacology
MH  - Rats
MH  - Receptors, Atrial Natriuretic Factor/genetics
MH  - Ventricular Myosins/genetics
OTO - NOTNLM
OT  - Cardiac hypertrophy
OT  - Chloride channel
OT  - Isoprenaline
EDAT- 2017/11/22 06:00
MHDA- 2017/12/27 06:00
CRDT- 2017/11/22 06:00
PHST- 2017/08/11 00:00 [received]
PHST- 2017/10/26 00:00 [revised]
PHST- 2017/11/15 00:00 [accepted]
PHST- 2017/11/22 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
PHST- 2017/11/22 06:00 [entrez]
AID - S0378-1119(17)31011-9 [pii]
AID - 10.1016/j.gene.2017.11.045 [doi]
PST - ppublish
SO  - Gene. 2018 Feb 5;642:335-342. doi: 10.1016/j.gene.2017.11.045. Epub 2017 Nov 20.