PMID- 29158195 OWN - NLM STAT- MEDLINE DCOM- 20181105 LR - 20181105 IS - 1878-5832 (Electronic) IS - 1359-6446 (Linking) VI - 23 IP - 3 DP - 2018 Mar TI - Understanding missing proteins: a functional perspective. PG - 644-651 LID - S1359-6446(17)30282-9 [pii] LID - 10.1016/j.drudis.2017.11.011 [doi] AB - A missing protein (MP) is an unconfirmed genetic sequence for which a protein product is not yet detected. Currently, MPs are tiered based on supporting evidence mainly in the form of protein existence (PE) classification. As we discuss here, this definition is overly restrictive because proteins go missing in day-to-day proteomics as a result of low abundance, lack of sequence specificity, splice variants, and so on. Thus, we propose a broader functional classification of MPs that complements PE classification, discuss major causes, and examine three corresponding solution tiers: biological, technical, and informatics. We assert that informatics-driven solutions would have a major role in resolving the MP problem (MPP). CI - Copyright (c) 2017 Elsevier Ltd. All rights reserved. FAU - Zhou, Longjian AU - Zhou L AD - School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China. FAU - Wong, Limsoon AU - Wong L AD - Department of Computer Science, National University of Singapore, 117417, Singapore; Department of Pathology, National University of Singapore, 119074, Singapore. Electronic address: wongls@comp.nus.edu.sg. FAU - Goh, Wilson Wen Bin AU - Goh WWB AD - School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China; Department of Computer Science, National University of Singapore, 117417, Singapore; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore. Electronic address: wilsongoh@ntu.edu.sg. LA - eng PT - Journal Article PT - Review DEP - 20171120 PL - England TA - Drug Discov Today JT - Drug discovery today JID - 9604391 RN - 0 (Proteins) RN - 0 (Proteome) SB - IM MH - Databases, Protein MH - Humans MH - Proteins/*metabolism MH - Proteome/*metabolism MH - Proteomics/methods EDAT- 2017/11/22 06:00 MHDA- 2018/11/06 06:00 CRDT- 2017/11/22 06:00 PHST- 2017/06/07 00:00 [received] PHST- 2017/10/24 00:00 [revised] PHST- 2017/11/13 00:00 [accepted] PHST- 2017/11/22 06:00 [pubmed] PHST- 2018/11/06 06:00 [medline] PHST- 2017/11/22 06:00 [entrez] AID - S1359-6446(17)30282-9 [pii] AID - 10.1016/j.drudis.2017.11.011 [doi] PST - ppublish SO - Drug Discov Today. 2018 Mar;23(3):644-651. doi: 10.1016/j.drudis.2017.11.011. Epub 2017 Nov 20.