PMID- 29158833 OWN - NLM STAT- MEDLINE DCOM- 20180319 LR - 20220801 IS - 1838-7640 (Electronic) IS - 1838-7640 (Linking) VI - 7 IP - 18 DP - 2017 TI - Human Umbilical Cord MSCs as New Cell Sources for Promoting Periodontal Regeneration in Inflammatory Periodontal Defect. PG - 4370-4382 LID - 10.7150/thno.19888 [doi] AB - Human periodontal ligament stem cells (hPDLSCs) transplantation represents a promising approach for periodontal regeneration; however, the cell source is limited due to the invasive procedure required for cell isolation. As human umbilical cord mesenchymal stem cells (hUCMSCs) can be harvested inexpensively and inexhaustibly, here we evaluated the regenerative potentials of hUCMSCs as compared with hPDLSCs to determine whether hUCMSCs could be used as new cell sources for periodontal regeneration. Methods The characteristics of hUCMSCs, including multi-differentiation ability and anti-inflammatory capability, were determined by comparison with hPDLSCs. We constructed cell aggregates (CA) using hUCMSCs and hPDLSCs respectively. Then hPDLSCs-CA and hUCMSCs-CA were combined with beta-tricalcium phosphate bioceramic (beta-TCP) respectively and their regenerative potentials were determined in a rat inflammatory periodontal defect model. Results hPDLSCs showed higher osteogenic differentiation potentials than hUCMSCs. Meanwhile, hUCMSCs showed higher extracellular matrix secretion and anti-inflammatory abilities than hPDLSCs. Similar to hPDLSCs, hUCMSCs were able to contribute to regeneration of both soft and hard periodontal tissues under inflammatory periodontitis condition. There were more newly formed bone and periodontal ligaments in hPDLSCs and hUCMSCs groups than in non-cell treated group. Moreover, no significant differences of regenerative promoting effects between hPDLSCs and hUCMSCs were found. Conclusion: hUCMSCs generated similar promoting effects on periodontal regeneration compared with hPDLSCs, and can be used as new cell sources for periodontal regeneration. FAU - Shang, Fengqing AU - Shang F AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China. AD - Department of Stomatology, the 306th Hospital of PLA, Beijing 100101, China. AD - State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. FAU - Liu, Shiyu AU - Liu S AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China. FAU - Ming, Leiguo AU - Ming L AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China. FAU - Tian, Rong AU - Tian R AD - Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China. FAU - Jin, Fang AU - Jin F AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. FAU - Ding, Yin AU - Ding Y AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. FAU - Zhang, Yongjie AU - Zhang Y AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China. FAU - Zhang, Hongmei AU - Zhang H AD - Department of Burns and Plastic surgery, Tangdu Hospital, Fourth Military University, Xi'an, Shaanxi, 710038, China. FAU - Deng, Zhihong AU - Deng Z AD - Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China. FAU - Jin, Yan AU - Jin Y AD - State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Center for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. AD - Research and Development Centre for Tissue Engineering, Fourth Military Medical University, Xi'an, Shaanxi, China. LA - eng PT - Journal Article DEP - 20170926 PL - Australia TA - Theranostics JT - Theranostics JID - 101552395 RN - 0 (Calcium Phosphates) RN - 0 (beta-tricalcium phosphate) SB - IM EIN - Theranostics. 2022 Jul 8;12(12):5332-5333. PMID: 35910810 MH - Adolescent MH - Adult MH - Animals MH - Calcium Phosphates/pharmacology MH - Cell Differentiation/drug effects/physiology MH - Cell Proliferation/drug effects/physiology MH - Cell Separation MH - Cells, Cultured MH - Child MH - Extracellular Matrix/drug effects/physiology MH - Female MH - Humans MH - Inflammation/pathology/*therapy MH - Mesenchymal Stem Cells/*cytology/drug effects MH - Osteogenesis/drug effects/physiology MH - Periodontal Ligament/*cytology/drug effects MH - Rats MH - Rats, Sprague-Dawley MH - Regeneration/drug effects/*physiology MH - Stem Cell Transplantation/methods MH - Umbilical Cord/*cytology/drug effects MH - Young Adult PMC - PMC5695137 OTO - NOTNLM OT - Cell therapy OT - Inflammation microenvironment. OT - Periodontal regeneration OT - UCMSC COIS- Competing Interests: The authors have declared that no competing interest exists. EDAT- 2017/11/22 06:00 MHDA- 2018/03/20 06:00 PMCR- 2017/01/01 CRDT- 2017/11/22 06:00 PHST- 2017/03/02 00:00 [received] PHST- 2017/08/18 00:00 [accepted] PHST- 2017/11/22 06:00 [entrez] PHST- 2017/11/22 06:00 [pubmed] PHST- 2018/03/20 06:00 [medline] PHST- 2017/01/01 00:00 [pmc-release] AID - thnov07p4370 [pii] AID - 10.7150/thno.19888 [doi] PST - epublish SO - Theranostics. 2017 Sep 26;7(18):4370-4382. doi: 10.7150/thno.19888. eCollection 2017.