PMID- 29160061
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 9
IP  - 49
DP  - 2017 Dec 13
TI  - A Dual Macrophage Targeting Nanovector for Delivery of Oligodeoxynucleotides To 
      Overcome Cancer-Associated Immunosuppression.
PG  - 42566-42576
LID - 10.1021/acsami.7b13594 [doi]
AB  - To overcome cancer-associated immunosuppression, we prepared a dual-targeting 
      vector to deliver CpG oligodeoxynucleotides (ODN) to macrophages. The 
      dual-targeting system composed of mannosylated carboxymethyl chitosan 
      (MCMC)/hyaluronan (HA) for macrophage targeting and protamine sulfate for ODN 
      complexation was prepared by self-assembly. The effects of ODN delivery on immune 
      cells was studied in J774A.1 cells. Due to the enhanced delivery efficiency, the 
      dual-targeting delivery system exhibits a higher immune stimulatory activity 
      compared with the monotargeting delivery system containing either MCMC or HA, 
      resulting in a dramatically enhanced secretion of proinflammatory cytokines and a 
      successful shift to activated macrophages (M1). Besides macrophages, the 
      influence of the delivery system on tumor cells (MCF-7) was also investigated. In 
      MCF-7 cells, the increased expressions of nuclear transcription factor-kappaB 
      (NF-kappaB), PIK3R3, and phosphorylated protein kinase B (p-Akt) caused by activated 
      NF-kappaB and phosphoinositide 3-kinase/Akt signalings were observed. Nevertheless, 
      upregulated Fas as well as Fas ligand (FasL) may induce Fas/FasL-mediated 
      apoptosis, which results in the increased expressions of caspases in tumor cells.
FAU - He, Xiao-Yan
AU  - He XY
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's 
      Republic of China.
FAU - Liu, Bo-Ya
AU  - Liu BY
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's 
      Republic of China.
FAU - Wu, Jin-Long
AU  - Wu JL
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's 
      Republic of China.
FAU - Ai, Shu-Lun
AU  - Ai SL
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's 
      Republic of China.
FAU - Zhuo, Ren-Xi
AU  - Zhuo RX
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's 
      Republic of China.
FAU - Cheng, Si-Xue
AU  - Cheng SX
AUID- ORCID: 0000-0001-9611-4421
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20171130
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (NF-kappa B)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Humans
MH  - *Macrophages
MH  - NF-kappa B
MH  - Neoplasms
MH  - Oligodeoxyribonucleotides
MH  - Phosphatidylinositol 3-Kinases
OTO - NOTNLM
OT  - CpG ODN
OT  - cancer treatment
OT  - gene delivery
OT  - immunotherapy
OT  - macrophage targeting
OT  - self-assembly
EDAT- 2017/11/22 06:00
MHDA- 2018/12/12 06:00
CRDT- 2017/11/22 06:00
PHST- 2017/11/22 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/11/22 06:00 [entrez]
AID - 10.1021/acsami.7b13594 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2017 Dec 13;9(49):42566-42576. doi: 
      10.1021/acsami.7b13594. Epub 2017 Nov 30.